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Information on EC 4.2.3.111 - (-)-alpha-terpineol synthase and Organism(s) Vitis vinifera and UniProt Accession Q6PWU2

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.111 (-)-alpha-terpineol synthase
IUBMB Comments
The enzyme has been characterized from Vitis vinifera (grape). Also forms some 1,8-cineole and traces of other monoterpenoids.
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This record set is specific for:
Vitis vinifera
UNIPROT: Q6PWU2
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The taxonomic range for the selected organisms is: Vitis vinifera
The enzyme appears in selected viruses and cellular organisms
Synonyms
tctps5, (-)-alpha-terpineol synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-terpineol synthase
-
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [cyclizing, (-)-alpha-terpineol-forming]
The enzyme has been characterized from Vitis vinifera (grape). Also forms some 1,8-cineole and traces of other monoterpenoids.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
(-)-alpha-terpineol + diphosphate
show the reaction diagram
-
products are 50.1% (-)-alpha-terpineol, 11.8% 1,8-cineole, 8.5% (+)-beta-pinene, plus lower amounts of additional monoterpenes
-
?
geranyl diphosphate + H2O
(-)-alpha-terpineol + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + H2O
(-)-alpha-terpineol + diphosphate
show the reaction diagram
-
-
-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
distribution of alpha-terpineol content in the 61 grapevine accessions
Manually annotated by BRENDA team
-
strongest signals with RNA from flower buds and strongly reduced transcript levels in pre- and postanthesis open flowers and in set flowers at early fruit onset
Manually annotated by BRENDA team
additional information
-
expression appears restricted to flowers
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
distribution of alpha-terpineol content in the 61 grapevine accessions
physiological function
alpha-terpineol is one of the main monoterpenes found in the grape berry (Vitis vinifera) with its concentrations varying in different varieties. Identification of associations between the alpha-terpineol synthase (alpha-TPS) gene, a regulator of alpha-terpineol metabolism, and alpha-terpineol content in different grapevine varieties
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ATESY_VITVI
590
0
68883
Swiss-Prot
Chloroplast (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A144S
naturally occuring non-synonymous single nuclear polymorphisms
E245G
naturally occuring non-synonymous single nuclear polymorphisms
E306Q
naturally occuring non-synonymous single nuclear polymorphisms
E492K
naturally occuring non-synonymous single nuclear polymorphisms
F317S
naturally occuring non-synonymous single nuclear polymorphisms
I38S
naturally occuring non-synonymous single nuclear polymorphisms
K230N
naturally occuring non-synonymous single nuclear polymorphisms
M185L
naturally occuring non-synonymous single nuclear polymorphisms
S441A
naturally occuring non-synonymous single nuclear polymorphisms
T519I
naturally occuring non-synonymous single nuclear polymorphisms
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
genotyping and identification of single nuclaer polymorphisms in the gene
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luecker, J.; Bowen, P.; Bohlmann, J.
Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Phytochemistry
65
2649-2659
2004
Vitis vinifera
Manually annotated by BRENDA team
Martin, D.M.; Bohlmann, J.
Identification of Vitis vinifera (-)-alpha-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase
Phytochemistry
65
1223-1229
2004
Vitis vinifera (Q6PWU2), Vitis vinifera
Manually annotated by BRENDA team
Yang, X.; Guo, Y.; Zhu, J.; Ma, N.; Sun, T.; Liu, Z.; Li, K.; Guo, X.
Associations between the alpha-terpineol synthase gene and alpha-terpineol content in different grapevine varieties
Biotechnol. Biotechnol. Equip.
31
1100-1105
2017
Vitis vinifera (Q6PWU2)
-
Manually annotated by BRENDA team