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Information on EC 4.2.3.110 - (+)-sabinene synthase and Organism(s) Salvia officinalis and UniProt Accession O81193

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.110 (+)-sabinene synthase
IUBMB Comments
Isolated from Salvia officinalis (sage). The recombinant enzyme gave 63% (+)-sabinene, 21% gamma-terpinene, and traces of other monoterpenoids. See EC 4.2.3.114 gamma-terpinene synthase.
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This record set is specific for:
Salvia officinalis
UNIPROT: O81193
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Word Map
The taxonomic range for the selected organisms is: Salvia officinalis
The enzyme appears in selected viruses and cellular organisms
Synonyms
(+)-sabinene synthase, mktps1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (+)-sabinene + diphosphate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [cyclizing, (+)-sabinene-forming]
Isolated from Salvia officinalis (sage). The recombinant enzyme gave 63% (+)-sabinene, 21% gamma-terpinene, and traces of other monoterpenoids. See EC 4.2.3.114 gamma-terpinene synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
141907-26-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
(+)-sabinene + diphosphate
show the reaction diagram
geranyl diphosphate + H2O
(+)-sabinene + diphosphate
show the reaction diagram
-
63% sabinene, 21% gamma-terpinene, 7% terpinolene, 6.5% limonene, and 2.5% myrcene
-
?
geranyl diphosphate
(+)-sabinene + diphosphate
show the reaction diagram
-
-
product is precursor of alpha- and beta-thujone
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
(+)-sabinene + diphosphate
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0074
geranyl diphosphate
pH 7.1, 31°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
epidermal gland of leaf
Manually annotated by BRENDA team
-
gene expression of monoterpene synthases and actual monoterpene formation is highest in young leaf. Steady increase of alpha- and beta-thujone concentration during the growing period of leaf
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
sabinene synthase is responsible for the first step in the formation of alpha- and beta-thujone
physiological function
sabinene synthase generates sabinene, the precursor of thujone. Drought stress induces changes in monoterpene contents
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SSS_SALOF
590
0
68942
Swiss-Prot
Chloroplast (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68490
x * 68490, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 68490, calculated
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
quantitative real-time PCR expression analysis
sequence comparisons and phylogenetic analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
daminozide significantly decreases gene expression of the monoterpene synthases sabinene synthase, borneol synthase, and 1,8-cineole synthase
enzyme expression is induced by drought stress (about twice within 14 days), quantitative real-time PCR expression analysis, overview
the foliar application of gibberellic acid increases gene expression of the monoterpene synthases sabinene synthase, borneol synthase, and 1,8-cineole synthase. The amounts of alpha- and beta-thujene are not transcriptionally regulated
accumulation of alpha- and beta-thujone is not transcriptionally regulated
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wise, M.L.; Savage, T.J.; Katahira, E.; Croteau, R.
Monoterpene synthases from common sage (Salvia officinalis). cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1,8-cineole synthase, and (+)-bornyl diphosphate synthase
J. Biol. Chem.
273
14891-14899
1998
Salvia officinalis (O81193)
Manually annotated by BRENDA team
Schmiderer, C.; Grausgruber-Groeger, S.; Grassi, P.; Steinborn, R.; Novak, J.
Influence of gibberellin and daminozide on the expression of terpene synthases and on monoterpenes in common sage (Salvia officinalis)
J. Plant Physiol.
167
779-786
2010
Salvia officinalis (O81193)
Manually annotated by BRENDA team
Peters, R.J.; Croteau, R.B.
Alternative termination chemistries utilized by monoterpene cyclases: chimeric analysis of bornyl diphosphate, 1,8-cineole, and sabinene synthases
Arch. Biochem. Biophys.
417
203-211
2003
Salvia officinalis (O81193), Salvia officinalis
Manually annotated by BRENDA team
Grausgruber-Groeger, S.; Schmiderer, C.; Steinborn, R.; Novak, J.
Seasonal influence on gene expression of monoterpene synthases in Salvia officinalis (Lamiaceae)
J. Plant Physiol.
169
353-359
2012
Salvia officinalis
Manually annotated by BRENDA team
Cao, Y.; Zhang, H.; Liu, H.; Liu, W.; Zhang, R.; Xian, M.; Liu, H.
Biosynthesis and production of sabinene current state and perspectives
Appl. Microbiol. Biotechnol.
102
1535-1544
2018
Citrus jambhiri, Citrus unshiu, Hedychium coronarium, Murraya koenigii (A0A1U9M7Y9), Phomopsis sp. EC-4, Picea sitchensis (F1CKJ1), Salvia officinalis (O81193), Salvia pomifera (A6XH06), Thuja plicata (R9WS05)
Manually annotated by BRENDA team
Radwan, A.; Kleinwaechter, M.; Selmar, D.
Impact of drought stress on specialised metabolism biosynthesis and the expression of monoterpene synthases in sage (Salvia officinalis)
Phytochemistry
141
20-26
2017
Salvia officinalis (O81193)
Manually annotated by BRENDA team