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Information on EC 4.2.3.109 - (-)-sabinene synthase
for references in articles please use BRENDA:EC4.2.3.109
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EC Tree 4 Lyases
4.2 Carbon-oxygen lyases
4.2.3 Acting on phosphates
4.2.3.109 (-)-sabinene synthase
IUBMB Comments
Requires Mg2+. Isolated from Pinus contorta (lodgepole pine) as cyclase I and from Conocephalum conicum (liverwort) .
The enzyme appears in viruses and cellular organisms
showSynonyms
(-)-sabinene synthase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (-)-sabinene + diphosphate
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [cyclizing, (-)-sabinene-forming]
Requires Mg2+. Isolated from Pinus contorta (lodgepole pine) as cyclase I [1] and from Conocephalum conicum (liverwort) [2].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + H2O
(-)-sabinene + diphosphate
Substrates: -
Products: 52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol
Products: 52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol
?
geranyl diphosphate
(-)-sabinene + diphosphate
-
Substrates: -
Products: sole products
Products: sole products
?
geranyl diphosphate
(-)-sabinene + diphosphate
-
Substrates: -
Products: wild-type produces 1.3% (+)-car-3-ene, 44.7% (-)-sabinene, 35.9% terpinolene
Products: wild-type produces 1.3% (+)-car-3-ene, 44.7% (-)-sabinene, 35.9% terpinolene
?
geranyl diphosphate
(-)-sabinene + diphosphate
-
Substrates: -
Products: products are 51.4% (-)-sabinene, 45.8% terpinolene, 2.8% alpha-pinene
Products: products are 51.4% (-)-sabinene, 45.8% terpinolene, 2.8% alpha-pinene
?
additional information
?
-
Substrates: no substrate: farnesyl diphosphate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: farnesyl diphosphate
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Mg2+
Mn2+
additional information
additional information
-
divalent cation absolutely required, with Mg2+ preferred. Activity with cations in decreasing order: Mg2+ > Fe2+ >Mn2+> Zn2+>Co2+ > Ni2+ >Cu2+
additional information
-
divalent cation absolutely required, Mg2+ is ineffective. Monovalent cation absolutely required, Li+ and Na+ are ineffective
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0002
geranyl diphosphate
temperature not specified in the publication, pH not specified in the publication
0.0202
geranyl diphosphate
-
mutant A595G/F596L/L599F, 30Ā°C, pH not specified in the publication
0.0316
geranyl diphosphate
-
mutant F596L, 30Ā°C, pH not specified in the publication
0.0357
geranyl diphosphate
-
wild-type, 30Ā°C, pH not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.001
geranyl diphosphate
temperature not specified in the publication, pH not specified in the publication
0.0352
geranyl diphosphate
-
mutant A595G/F596L/L599F, 30Ā°C, pH not specified in the publication
0.0825
geranyl diphosphate
-
mutant F596L, 30Ā°C, pH not specified in the publication
0.545
geranyl diphosphate
-
wild-type, 30Ā°C, pH not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0017
geranyl diphosphate
-
mutant A595G/F596L/L599F, 30Ā°C, pH not specified in the publication
0.0027
geranyl diphosphate
-
mutant F596L, 30Ā°C, pH not specified in the publication
0.0153
geranyl diphosphate
-
wild-type, 30Ā°C, pH not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
brenda
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
brenda
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ATERP_PINCO
616
0
70590
Swiss-Prot
Chloroplast (Reliability: 2)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A595G
-
shift in product profile, mutant produces 2.4% (+)-car-3-ene, 39% (-)-sabinene, 33.3% terpinolene
A595G/F596L/L599F
-
shift in product profile, mutant produces 42.3% (+)-car-3-ene, 7.3% (-)-sabinene, 20.1% terpinolene
F596E
-
shift in product profile, mutant produces 0.5% (+)-car-3-ene, 10.2% (-)-sabinene, 5.7% terpinolene, 70.9% limonene
F596G
-
shift in product profile, mutant produces 10.0% (+)-car-3-ene, 11.2% (-)-sabinene, 17.3% terpinolene, 27.0% myrcene, 17.0% limonene
F596H
-
shift in product profile, mutant produces 2.1% (+)-car-3-ene, 46.5% (-)-sabinene, 27.1% terpinolene
F596L
-
shift in product profile, mutant produces 28.6% (+)-car-3-ene, 42.8% (-)-sabinene, 36.4% terpinolene
L599F
-
shift in product profile, mutant produces 1.5% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene
S589A
-
shift in product profile, mutant produces 1.2% (+)-car-3-ene, 42.9% (-)-sabinene, 35.5% terpinolene
additional information
-
construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by or (-)-sabinene. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Adam, K.P.; Croteau, R.
Monoterpene biosynthesis in the liverwort Conocephalum conicum: demonstration of sabinene synthase and bornyl diphosphate synthase
Phytochemistry
49
475-480
1998
Conocephalum conicum
brenda
Savage, T.J.; Ichii, H.; Hume, S.D.; Little, D.B.; Croteau, R.
Monoterpene synthases from Gymnosperms and Angiosperms: stereospecificity and inactivation by cysteinyl- and arginyl-directed modifying reagents
Arch. Biochem. Biophys.
269
4012-4020
1994
Pinus contorta
-
brenda
Chen, F.; Ro, D.K.; Petri, J.; Gershenzon, J.; Bohlmann, J.; Pichersky, E.; Tholl, D.
Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole
Plant Physiol.
135
1956-1966
2004
Arabidopsis thaliana (P0DI76), Arabidopsis thaliana (P0DI77)
brenda
Roach, C.R.; Hall, D.E.; Zerbe, P.; Bohlmann, J.
Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a Sitka spruce monoterpene synthase gene family associated with weevil resistance
J. Biol. Chem.
289
23859-23869
2014
Picea sitchensis
brenda
EXTERNAL LINKS (specific for EC-Number 4.2.3.109)
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Release 2023.1 (January 2023)
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