Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
O-phospho-L-homoserine + H2O = L-threonine + phosphate
O-phospho-L-homoserine + H2O = L-threonine + phosphate
mechanism
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
mechanism
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
mechanism
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
mechanism
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
reaction proceeds via phospate removal and isomerization from primary to secondary alcohol
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
mechanism of phosphohomoserine dephosphorylation/deamination activity
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
inhibitor studies concerning mechanism, model of stepwise catalytic mechanism
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
reaction proceeds via phosphate removal and isomerization from primary to secondary alcohol
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
reaction proceeds via phosphate removal and isomerization from primary to secondary alcohol
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
reaction proceeds via phosphate removal and isomerization from primary to secondary alcohol
O-phospho-L-homoserine + H2O = L-threonine + phosphate
analyzed crystal structure gives new insights into the catalytic mechanism of threonine synthase in general, specifically by suggesting the direct involvement of the phosphate moiety of the cofactor, rather than the inorganic phosphate product, in transferring a proton from C4 to Cgamma in the formation of the alphabeta-unsaturated aldimine
O-phospho-L-homoserine + H2O = L-threonine + phosphate
the phosphate ion released from O-phospho-L-homoserine by gamma-elimination acts as the base catalyst for the addition of water at Cbeta of the alpha-aminocrotonate aldimine, thereby providing the basis of the reaction specificity. The phosphate ion also accelerates the protonation/deprotonation at Cgamma
O-phospho-L-homoserine + H2O = L-threonine + phosphate
analyzed crystal structure gives new insights into the catalytic mechanism of threonine synthase in general, specifically by suggesting the direct involvement of the phosphate moiety of the cofactor, rather than the inorganic phosphate product, in transferring a proton from C4 to Cgamma in the formation of the alphabeta-unsaturated aldimine
-
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
the phosphate ion released from O-phospho-L-homoserine by gamma-elimination acts as the base catalyst for the addition of water at Cbeta of the alpha-aminocrotonate aldimine, thereby providing the basis of the reaction specificity. The phosphate ion also accelerates the protonation/deprotonation at Cgamma
-
-
O-phospho-L-homoserine + H2O = L-threonine + phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DL-3-chloroalanine
pyruvate + NH3 + HCl
-
beta-elimination
-
ir
DL-vinylglycine + H2O
L-threonine
-
-
-
ir
L-allo-threonine + H2O
2-oxobutyrate + NH3
-
beta-elimination
-
ir
L-serine
pyruvate + NH3
-
beta-elimination
-
ir
L-vinylglycine + phosphate + H2O
L-threonine + phosphate
O-phospho-L-homoserine + H2O
L-threonine + phosphate
phosphohomoserine
2-oxobutyrate + phosphate + ?
-
bypass of threonine in isoleucine biosynthesis
-
ir
threonine
2-oxobutyrate + NH3
additional information
?
-
L-vinylglycine + phosphate + H2O
L-threonine + phosphate
in the presence of phosphate, L-threonine is formed with kcat and reaction specificity comparable with those when O-phospho-L-homoserine is used as the substrate. In the absence of phosphate or when sulfate is used in place of phosphate, only the side reaction product, alpha-ketobutyrate, is formed. Compared with the more acidic sulfate ion, the phosphate ion decreases the energy levels of the transition states of the addition of water at the Cbeta of the PLP-alpha-aminocrotonate aldimine and the transaldimination to form L-threonine. Threonine synthase is in the closed form, when the substrate and the intermediates are bound to the enzyme
-
-
?
L-vinylglycine + phosphate + H2O
L-threonine + phosphate
in the presence of phosphate, L-threonine is formed with kcat and reaction specificity comparable with those when O-phospho-L-homoserine is used as the substrate. In the absence of phosphate or when sulfate is used in place of phosphate, only the side reaction product, alpha-ketobutyrate, is formed. Compared with the more acidic sulfate ion, the phosphate ion decreases the energy levels of the transition states of the addition of water at the Cbeta of the PLP-alpha-aminocrotonate aldimine and the transaldimination to form L-threonine. Threonine synthase is in the closed form, when the substrate and the intermediates are bound to the enzyme
-
-
?
O-phospho-L-homoserine
?
-
involved in allocation of phosphohomoserine between cystathione and threonine pathways
-
-
?
O-phospho-L-homoserine
?
-
studies on regulatory properties
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
threonine synthesis in eukaryotes
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
enzyme at the metabolic branch point between methionine and threonine biosynthesis
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
last reaction in the synthesis of threonine from aspartate
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
threonine synthesis in eukaryotes
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
threonine synthesis in eukaryotes
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
ir
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
final step of threonine biosynthesis
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
reaction is 99% specific for L-threonine formation. Minor product is 2-ketobutanoate
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
reaction is 99% specific for L-threonine formation. Minor product is 2-ketobutanoate
-
?
threonine
2-oxobutyrate + NH3
-
-
-
ir
threonine
2-oxobutyrate + NH3
-
genetic evidence for identity of protein with both activities
-
ir
threonine
2-oxobutyrate + NH3
-
beta-elimination
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
half-transamination reactions
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
O-phospho-L-homoserine + H2O
L-threonine + phosphate
O-phospho-L-homoserine
?
-
involved in allocation of phosphohomoserine between cystathione and threonine pathways
-
-
?
O-phospho-L-homoserine
?
-
studies on regulatory properties
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
threonine synthesis in eukaryotes
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
enzyme at the metabolic branch point between methionine and threonine biosynthesis
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
last reaction in the synthesis of threonine from aspartate
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
threonine synthesis in eukaryotes
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
threonine synthesis in eukaryotes
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
final step of threonine biosynthesis
-
?
O-phospho-L-homoserine + H2O
L-threonine + phosphate
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DL-2-amino-3[(phosphonomethyl)thio]propionic acid
-
Ki: 0.057 mM, kinact: 1.44 min-1
DL-E-2-amino-5-phosphono-4-pentenoic acid
-
Ki: 0.54 mM
GMP
-
25% inhibition at 67 mM
IMP
-
23% inhibition at 67 mM
KCN
-
22% inhibition at 1 mM
L-2,3-methanohomoserine phosphate
-
Ki: 0.01 mM
L-2-amino-3[(phosphonomethyl)thio]propionic acid
L-3-hydroxyhomoserine
-
Ki: 0.05 mM
L-threo-3-hydroxyhomoserine
NH2OH
-
65% inhibition at 1 mM
O-phospho-DL-threonine
-
-
phospho-threonine
-
35% inhibition at 10 mM
phosphonovaleric acid
-
Ki: 0.031 mM
Vinylglycine
-
22% inhibition at 10 mM
AMP
-
same binding site as S-adenosylmethionine
AMP
-
50% inhibition at 40 mM, reversible, depends on phosphohomoserine concentration
L-2-amino-3[(phosphonomethyl)thio]propionic acid
-
Ki: 0.00011 mM, "slow, tight" inhibition kinetics
L-2-amino-3[(phosphonomethyl)thio]propionic acid
-
Ki: 0.011 mM
L-cysteine
-
-
L-cysteine
-
stereospecific, inhibits S-adenosylmethionine activation
L-cysteine
-
strong inhibition at 0.5 mM
L-cysteine
-
26% inhibition at 1 mM, reversible
L-cysteine
-
85% inhibition at 35 mM
L-cysteine
-
80% inhibition at 1 mM in presence of S-adenosylmethionine
L-threo-3-hydroxyhomoserine
-
marked inhibition at 5 mM, abolished by 60 mM Mg2+
L-threo-3-hydroxyhomoserine
-
Ki: 0.006 mM
phosphate
-
50% inhibition at 1 mM, competitive, reversible, depends on phosphohomoserine concentration
phosphate
-
40% inhibition at 50 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine
-
S-adenosyl-L-methionine
-
activates
S-adenosylmethionine
activates
S-adenosylmethionine
-
affects both Km and Vmax by allosteric and cooperative transition of enzyme, two mol per mol enzyme, 25fold decrease of Km at 0.06 mM
S-adenosylmethionine
-
3-20fold increase of specific activity in various recombinant enzymes at 0.2 mM
S-adenosylmethionine
-
same binding site as inhibitor AMP, 85fold increase of activity, maximum activity at 0.06-0.25 mM
S-adenosylmethionine
-
allosteric activator, 14fold increase of Vmax, 8% decrease of substrate Km at 0.5 mM
S-adenosylmethionine
-
allosteric activator, maximal activation at 0.1-0.2 mM
S-adenosylmethionine
-
allosteric activator, 4fold increase of Vmax, 3.3fold decrease of substrate Km at 0.4 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.2.3.1 threonine synthase
more
top
