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Information on EC 4.2.2.9 - pectate disaccharide-lyase
for references in articles please use BRENDA:EC4.2.2.9
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EC Tree 4 Lyases
4.2 Carbon-oxygen lyases
4.2.2 Acting on polysaccharides
4.2.2.9 pectate disaccharide-lyase
IUBMB Comments
The enzyme catalyses the eliminative cleavage of an unsaturated disaccharide from the reducing end of homogalacturonan (the backbone of smooth regions of pectate, also known as de-esterified pectin).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 4.2.2.9
- pectin
- polygalacturonic
-
pectinolytic
- endopolygalacturonase
- polygalacturonases
- oligogalacturonates
- chrysanthemi
- exopgs
-
macer
-
digalacturonic
-
endo-pectate
- tubingensis
- trigalacturonate
- pectinesterase
- pectinases
-
exo-poly-alpha-d-galacturonosidase
-
pectin-degrading
-
methyl-esterified
-
soft-rot
-
lycopersici
- industry
Reaction Schemes
showSynonyms
exopolygalacturonase, exo-pectate lyase, exopectate lyase, exopolygalacturonate lyase, exopl, exopolygalacturonate lyase x, exopectate lyase w, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
exo-PATE
-
-
-
-
exo-PGL
-
-
-
-
exocleaving pectate lyase
exopectate lyase
exopectate pectate lyase
exopectic acid transeliminase
-
-
-
-
ExoPL
-
-
-
-
Exopolygalacturonate lyase
-
-
-
-
lyase, exopolygalacturonate
-
-
-
-
PelL
-
-
-
-
PelW
PelX
poly(1,4-alpha-D-galacturonide) exo-lyase
-
-
-
-
exopectate lyase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(1,4-alpha-D-galacturonosyl)n = (1,4-alpha-D-galacturonosyl)n-2 + 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate
(1,4-alpha-D-galacturonosyl)n = (1,4-alpha-D-galacturonosyl)n-2 + 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate
-
-
-
-
(1,4-alpha-D-galacturonosyl)n = (1,4-alpha-D-galacturonosyl)n-2 + 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate
trans-elimination mechanism
Clostridium multifermentans
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
elimination
elimination
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-galacturonan reducing-end-disaccharide-lyase
The enzyme catalyses the eliminative cleavage of an unsaturated disaccharide from the reducing end of homogalacturonan (the backbone of smooth regions of pectate, also known as de-esterified pectin).
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CAS REGISTRY NUMBER
COMMENTARY
37290-87-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-methyl-di-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked, 36% of the activity with di-D-galacturonate
Products: -
Products: -
?
2-methyl-di-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked, 35% of the activity with di-D-galacturonate
Products: -
Products: -
?
2-methyl-tri-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked, 19% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate
Products: -
Products: -
?
DELTA4,5-unsaturated tri-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked, low activity with PelX
Products: -
Products: -
?
heptagalacturonate
?
Substrates: better substrate than polygalacturonate
Products: -
Products: -
?
poly-D-galacturonic acid
?
-
Substrates: pectin from citrus
Products: -
Products: -
?
1-methyl-tri-D-galacturonate
?
-
Substrates: best substrate, alpha-(1-4)-linked
Products: -
Products: -
?
1-methyl-tri-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked
Products: -
Products: -
?
3-methyl-tri-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked, 18% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate
Products: -
Products: -
?
3-methyl-tri-D-galacturonate
?
-
Substrates: poor substrate only for PelW, not for PelX
Products: -
Products: -
?
hexagalacturonic acid
?
Substrates: better substrate than polygalacturonate
Products: -
Products: -
?
hexagalacturonic acid
?
-
Substrates: 51% of the activity with low molecular polygalacturonic acid
Products: -
Products: -
?
pectic acid
4,5-unsaturated digalacturonic acid
-
Substrates: acid-soluble pectic acid and acid-insoluble pectic acid
Products: -
Products: -
?
pectic acid
4,5-unsaturated digalacturonic acid
-
Substrates: acid-soluble pectic acid and acid-insoluble pectic acid
Products: -
Products: -
?
pectic acid
4,5-unsaturated digalacturonic acid
-
Substrates: -
Products: -
Products: -
?
pentagalacturonic acid
?
Substrates: better substrate than polygalacturonate
Products: -
Products: -
?
pentagalacturonic acid
?
Substrates: better substrate than polygalacturonate
Products: -
Products: -
?
pentagalacturonic acid
?
-
Substrates: 60% of the activity with low molecular polygalacturonic acid
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
Clostridium multifermentans
-
Substrates: -
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
Clostridium multifermentans
-
Substrates: -
Products: the major end product is O-(4-deoxy-beta-L-threo-hexopyranos-4-enyluronic acid)-(1,4)-D-galacturonic acid
Products: the major end product is O-(4-deoxy-beta-L-threo-hexopyranos-4-enyluronic acid)-(1,4)-D-galacturonic acid
?
polygalacturonate
unsaturated digalacturonate
Clostridium multifermentans
-
Substrates: -
Products: plus one molecule of digalacturonate originating from the nonreducing end of the chain
Products: plus one molecule of digalacturonate originating from the nonreducing end of the chain
?
polygalacturonate
unsaturated digalacturonate
-
Substrates: -
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
Substrates: -
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
Substrates: -
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
-
Substrates: -
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
-
Substrates: -
Products: 4,5-unsaturated digalacturonic acid
Products: 4,5-unsaturated digalacturonic acid
?
polygalacturonate
unsaturated digalacturonate
-
Substrates: low molecular polygalacturonic acid, very low activity with high molecular polygalacturonic acid
Products: -
Products: -
?
polygalacturonate
unsaturated digalacturonate
-
Substrates: -
Products: -
Products: -
?
tetragalacturonic acid
?
Substrates: maximal activity, better substrate than polygalacturonate
Products: -
Products: -
?
tetragalacturonic acid
?
Substrates: maximal activity, better substrate than polygalacturonate
Products: -
Products: -
?
tetragalacturonic acid
?
-
Substrates: maximal activity, better substrate than polygalacturonate
Products: -
Products: -
?
tetragalacturonic acid
?
-
Substrates: maximal activity, better substrate than polygalacturonate
Products: -
Products: -
?
tetragalacturonic acid
?
-
Substrates: 52% of the activity with low molecular polygalacturonic acid
Products: -
Products: -
?
tri-D-galacturonate
?
-
Substrates: alpha-(1-4)-linked, best substrate for both enzyme types
Products: -
Products: -
?
trigalacturonic acid
?
Substrates: poor substrate
Products: -
Products: -
?
trigalacturonic acid
?
-
Substrates: 248% of the activity with low molecular polygalacturonic acid
Products: -
Products: -
?
additional information
?
-
Clostridium multifermentans
-
Substrates: no activity with pectin of high methoxyl content
Products: -
Products: -
?
additional information
?
-
Substrates: does not cleave digalacturonate
Products: -
Products: -
?
additional information
?
-
-
Substrates: does not cleave digalacturonate
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview, no activity for both enzyme types with monomethyl-esterified digalacturonate substrates and with 2-methyl-tri-G-galacturonate
Products: -
Products: -
?
additional information
?
-
Substrates: does not cleave digalacturonate
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Fe2+
activates, 85% of maximal activity in 0.1 M TES, pH 7.4, 0.1 mM Fe2+
Mg2+
Mn2+
Na+
Sr2+
Ca2+
Clostridium multifermentans
-
greatest stimulation of divalent cations tested, Km: 0.06 mM
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
PelN
presence of PelN has an synergistic effect on PelX activity
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
Clostridium multifermentans
-
Km for polygalacturonate is 0.114% on the basis of dry, ash-free polygalacturonic acid, in presence of 0.5 mM CaCl2
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.311
-
fractionated enzyme in supernatant fluid, no activity in crude cell extract
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 8.5
Clostridium multifermentans
-
pH 5: no activity, pH 6.0: 15% of maximal activity, pH 7.0: 40% of maximal activity, pH 8.5: maximal activity
7 - 9
Clostridium multifermentans
-
pH 7: about 40% of maximal activity, pH 9: about 85% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
2 different enzyme types: exopolygalacturonate lyase X, i.e. Pel X, and exopectate lyase W, i.e. PelW
-
-
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
Show AA Sequence and Transmembrane Helices (163 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
Clostridium multifermentans
-
38°C, most stable at
34111
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