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IUBMB CommentsDoes not act on N,O-desulfated glucosamine or N-acetyl-O-sulfated glucosamine linkages.
The taxonomic range for the selected organisms is: Bacteroides stercoris
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
heparitinase, heparinase iii, heparitinase i, hep i, hepiii, heparin lyase iii, heparan sulfate lyase, heparitin-sulfate lyase,
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heparan sulfate lyase
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heparin lyase III
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heparin sulfate eliminase
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heparin-sulfate eliminase
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heparitin sulfate lyase
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heparitin-sulfate lyase
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lyase, heparin sulfate
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elimination of sulfate
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heparin-sulfate lyase
Does not act on N,O-desulfated glucosamine or N-acetyl-O-sulfated glucosamine linkages.
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heparan sulfate
n alpha-deltaUA-glcNAc
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major product
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?
hyaluronic acid
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?
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additional information
optimal condition: 350 mM NaCl
additional information
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optimal condition: 350 mM NaCl
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additional information
heparan sulfate
additional information
heparan sulfate
0.08808 mg/ml
additional information
heparan sulfate
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0.08808 mg/ml
additional information
heparin
0.42275 mg/ml
additional information
heparin
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0.42275 mg/ml
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1.7
hyaluronic acid as substrate
21.46
heparin as substrate
32.3
heparan sulfate (porcine intestine) as substrate
51.81
heparan sulfate (bovine kidney) as substrate
additional information
no activity with acharan sulfate, chondroitin sulfate A, and chondroitin sulfate C
additional information
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no activity with acharan sulfate, chondroitin sulfate A, and chondroitin sulfate C
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42 - 48
activity above 90%
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8.65
calculated for mature protein
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UniProt
brenda
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HEPC_BACSE
666
0
77325
Swiss-Prot
-
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74930
x * 74930, calculated for mature protein
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?
x * 74930, calculated for mature protein
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C352A
87% wild-type activity (heparan sulfate), 72% wild-type activity (heparin)
C474A
32% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin)
C577A
98% wild-type activity (heparan sulfate), 72% wild-type activity (heparin)
C624A
24% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin)
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additional information
about 20% activity after 1 h at 45°C
additional information
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about 20% activity after 1 h at 45°C
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immobilized metal ion affinity chromatography
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His-tagged version expressed in Escherichia coli BL21(DE3)
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Hyun, Y.J.; Lee, J.H.; Kim, D.H.
Cloning, overexpression, and characterization of recombinant heparinase III from Bacteroides stercoris HJ-15
Appl. Microbiol. Biotechnol.
86
879-890
2010
Bacteroides stercoris (C7EXL6), Bacteroides stercoris, Bacteroides stercoris HJ-15 (C7EXL6), Bacteroides stercoris HJ-15
brenda
4.2.2.8 heparin-sulfate lyase
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The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). 
