Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

Reference on EC 4.2.2.7 - heparin lyase

Please use the Reference Search for a specific query.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, V.C.; Linhard, R.J.; Bernstein, H.; Cooney, Ch.L.; Langer, R.
Purification and characterization of heparinase from Flavobacterium heparinum
J. Biol. Chem.
260
1849-1857
1985
Pedobacter heparinus
Manually annotated by BRENDA team
Nakamura, T.; Shibata, Y.; Fujimura, S.
Purification and properties of Bacteroides heparinolyticus heparinase (heparin lyase, EC 4.2.2.7)
J. Clin. Microbiol.
26
1070-1071
1988
Bacteroides heparinolyticus
Manually annotated by BRENDA team
Silverberg, I.; Havsmark, B.; Fransson, L.A.
The substrate specificity of heparan sulphate lyase and heparin lyase from Flavobacterium heparinum
Carbohydr. Res.
137
227-238
1985
Pedobacter heparinus
-
Manually annotated by BRENDA team
Arbogast, B.; Hopwood, J.J.; Dorfman, A.
Heparinase activity in rat liver
Biochem. Biophys. Res. Commun.
75
610-617
1977
Rattus norvegicus
Manually annotated by BRENDA team
Silva, M.E.; Dietrich, C.P.
Structure of heparin. Characterization of the products formed from heparin by the action of a heparinase and a heparitinase from Flavobacterium heparinum
J. Biol. Chem.
250
6841-6846
1975
Pedobacter heparinus
Manually annotated by BRENDA team
Dietrich, C.P.; Silva, M.E.; Michelacci, Y.M.
Sequential degradation of heparin in Flavobacterium heparinum. Purification and properties of five enzymes involved in heparin degradation
J. Biol. Chem.
248
6408-6415
1973
Pedobacter heparinus
Manually annotated by BRENDA team
Hovingh, P.; Linker, A.
The enzymatic degradation of heparin and heparitin sulfate. 3. Purification of a heparitinase and a heparinase from flavobacteria
J. Biol. Chem.
245
6170-6175
1970
Flavobacteriia
Manually annotated by BRENDA team
Yang, V.C.; Bernstein, H.; Langer, R.
Large scale production of heparinase
Biotechnol. Prog.
3
27-30
1987
Pedobacter heparinus
-
Manually annotated by BRENDA team
Linhardt, R.J.; Fitzgerald, G.L.; Cooney, Ch.L.
Mode of action of heparin lyase on heparin
Biochim. Biophys. Acta
702
197-203
1982
Pedobacter heparinus
Manually annotated by BRENDA team
Bernstein, H.; Yang, V.C.; Langer, R.
An investigation of heparinase immobilization
Appl. Biochem. Biotechnol.
16
129-143
1987
Pedobacter heparinus
Manually annotated by BRENDA team
Linhardt, R.J.; Cooney, C.L.; Tapper, D.; Zannetos, C.A.; Larsen, A.K.; Langer, R.
An immobilized microbial heparinase for blood deheparinization
Appl. Biochem. Biotechnol.
9
41-55
1984
Pedobacter heparinus
Manually annotated by BRENDA team
Joubert, J.J.; Pitout, M.J.
A constitutive heparinase in a Flavobacterium sp.
Experientia
41
1541
1985
Flavobacterium sp.
-
Manually annotated by BRENDA team
Cerbelaud, E.C.; Conway, L.J.; Galliher, P.M.; Langer, R.S.; Cooney, C.L.
Sulfur regulation of heparinase and sulfatases in Flavobacterium heparinum
Appl. Environ. Microbiol.
51
640-646
1986
Pedobacter heparinus
Manually annotated by BRENDA team
Bernstein, H.; Yang, V.C.; Langer, R.
Distribution of heparinase covalently immobilized to agarose: Experimental and theoretical studies
Biotechnol. Bioeng.
30
196-207
1987
Pedobacter heparinus
Manually annotated by BRENDA team
Yang, V.C.; Bernstein, H.; Cooney, Ch.L.; Langer, R.
Large scale preparation and characterization of mucopolysaccharase contamination free heparinase
Appl. Biochem. Biotechnol.
16
35-50
1987
Pedobacter heparinus
Manually annotated by BRENDA team
Yoshida, E.; Sakai, K.; Tokuyama, S.; Miyazono, H.; Maruyama, H.; Morikawa, K.; Yoshida, K.; Tahara, Y.
Purification and characterization of heparinase that degrades both heparin and heparan sulfate from Bacillus circulans
Biosci. Biotechnol. Biochem.
66
1181-1184
2002
Niallia circulans, Niallia circulans HpT298
Manually annotated by BRENDA team
Yoshida, E.; Arakawa, S.; Matsunaga, T.; Toriumi, S.; Tokuyama, S.; Morikawa, K.; Tahara, Y.
Cloning, sequencing, and expression of the gene from Bacillus circulans that codes for a heparinase that degrades both heparin and heparan sulfate
Biosci. Biotechnol. Biochem.
66
1873-1879
2002
Niallia circulans (Q8KKH9), Niallia circulans, Niallia circulans HpT298 (Q8KKH9)
Manually annotated by BRENDA team
Kim, B.T.; Kim, W.S.; Kim, Y.S.; Linhardt, R.J.; Kim, D.H.
Purification and characterization of a novel heparinase from Bacteroides stercoris HJ-15
J. Biochem.
128
323-328
2000
Bacteroides stercoris, Bacteroides stercoris HJ-15
Manually annotated by BRENDA team
Yapeng, C.; Ningguo, G.; Xiulan, C.; Jing, Y.; Shijun, Q.; Shuzheng, Z.
Rapid purification, characterization and substrate specificity of heparinase from a novel species of Sphingobacterium
J. Biochem.
134
365-371
2003
Sphingobacterium sp.
Manually annotated by BRENDA team
Liu, D.; Shriver, Z.; Godavarti, R.; Venkataraman, G.; Sasisekharan, R.
The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymic activity
J. Biol. Chem.
274
4089-4095
1999
Pedobacter heparinus
Manually annotated by BRENDA team
Yu, G.; LeBrun, L.; Gunay, N.S.; Hoppensteadt, D.; Walenga, J.M.; Fareed, J.; Linhardt, R.J.
Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site
Thromb. Res.
100
549-556
2000
Pedobacter heparinus
Manually annotated by BRENDA team
Shaya, D.; Li, Y.; Cygler, M.
Crystallization and preliminary X-ray analysis of heparinase II from Pedobacter heparinus
Acta Crystallogr. Sect. D
60
1644-1646
2004
Pedobacter heparinus
Manually annotated by BRENDA team
Chem, Y.; Xing, X.H.; Lou, K.
Construction of recombinant Escherichia coli for over-production of soluble heparinase I by fusion to maltose-binding protein
Biochem. Eng. J.
23
155-159
2005
Pedobacter heparinus
-
Manually annotated by BRENDA team
Kim, W.S.; Kim, B.T.; et.al.
Purification of heparin lyase I from Bacteroides stercoris HJ-15
J. Biochem. Mol. Biol.
37
684-690
2004
Bacteroides stercoris, Bacteroides stercoris HJ-15
Manually annotated by BRENDA team
Wei, Z.; Lyon, M.; Gallagher, J.T.
Distinct substrate specificities of bacterial heparinases against N-unsubstituted glucosamine residues in heparan sulfate
J. Biol. Chem.
280
15742-15748
2005
Pedobacter heparinus
Manually annotated by BRENDA team
Shaya, D.; Tocilj, A.; et.al.
Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product
J. Biol. Chem.
281
15525-15535
2006
Pedobacter heparinus
Manually annotated by BRENDA team
Luo, Y.; Huang, X.; McKeehan, W.L.
High yield, purity and activity of soluble recombinant Bacteroides thetaiotaomicron GST-heparinase I from Escherichia coli
Arch. Biochem. Biophys.
460
17-24
2007
Bacteroides thetaiotaomicron
Manually annotated by BRENDA team
Ma, X.; Wang, Z.; Li, S.; Shen, Q.; Yuan, Q.
Effect of CaCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum
J. Chromatogr. B
843
209-215
2006
Pedobacter heparinus
Manually annotated by BRENDA team
Ahn, S.C.; Kim, B.Y.; Oh, W.K.; Park, Y.M.; Kim, H.M.; Ahn, J.S.
Colorimetric heparinase assay for alternative anti-metastatic activity
Life Sci.
79
1661-1665
2006
Pedobacter heparinus
Manually annotated by BRENDA team
Manton, K.J.; Leong, D.F.; Cool, S.M.; Nurcombe, V.
Disruption of heparan and chondroitin sulfate signaling enhances mesenchymal stem cell-derived osteogenic differentiation via bone morphogenetic protein signaling pathways
Stem Cells
25
2845-2854
2007
Homo sapiens
Manually annotated by BRENDA team
Banga, J.; Tripathi, C.K.; Bihari, V.
Growth and enzyme production kinetics of a heparinase-producing fungal isolate
Med. Chem. Res.
17
85-93
2008
Aspergillus oryzae, Pedobacter heparinus
-
Manually annotated by BRENDA team
Hyun, Y.J.; Lee, K.S.; Kim, D.H.
Cloning, expression and characterization of acharan sulfate-degrading heparin lyase II from Bacteroides stercoris HJ-15
J. Appl. Microbiol.
108
226-235
2010
Bacteroides stercoris (C0JBW5), Bacteroides stercoris HJ-15 (C0JBW5), Bacteroides stercoris HJ-15
Manually annotated by BRENDA team
Han, Y.H.; Garron, M.L.; Kim, H.Y.; Kim, W.S.; Zhang, Z.; Ryu, K.S.; Shaya, D.; Xiao, Z.; Cheong, C.; Kim, Y.S.; Linhardt, R.J.; Jeon, Y.H.; Cygler, M.
Structural snapshots of heparin depolymerization by heparin lyase I
J. Biol. Chem.
284
34019-34027
2009
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron WAL2926
Manually annotated by BRENDA team
Shaya, D.; Zhao, W.; Garron, M.L.; Xiao, Z.; Cui, Q.; Zhang, Z.; Sulea, T.; Linhardt, R.J.; Cygler, M.
Catalytic mechanism of heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate
J. Biol. Chem.
285
20051-20061
2010
Pedobacter heparinus
Manually annotated by BRENDA team
Banga, J.; Tripathi, C.K.M.
Purification and characterization of a novel heparin degrading enzyme from Aspergillus flavus (MTCC-8654)
Appl. Biochem. Biotechnol.
160
1004-1016
2010
Aspergillus flavus, Aspergillus flavus MTCC-8654
Manually annotated by BRENDA team
Raman, K.; Kuberan, B.
Differential effects of heparitinase I and heparitinase III on endothelial tube formation in vitro
Biochem. Biophys. Res. Commun.
398
191-193
2010
Pedobacter heparinus
Manually annotated by BRENDA team
Zhao, W.; Garron, M.L.; Yang, B.; Xiao, Z.; Esko, J.D.; Cygler, M.; Linhardt, R.J.
Asparagine 405 of heparin lyase II prevents the cleavage of glycosidic linkages proximate to a 3-O-sulfoglucosamine residue
FEBS Lett.
585
2461-2466
2011
Pedobacter heparinus
Manually annotated by BRENDA team
Xiao, Z.; Zhao, W.; Yang, B.; Zhang, Z.; Guan, H.; Linhardt, R.J.
Heparinase 1 selectivity for the 3,6-di-O-sulfo-2-deoxy-2-sulfamido-alpha-D-glucopyranose (1,4) 2-O-sulfo-alpha-L-idopyranosyluronic acid (GlcNS3S6S-IdoA2S) linkages
Glycobiology
21
13-22
2011
Pedobacter heparinus
Manually annotated by BRENDA team
Wu, J.; Zhang, C.; Mei, X.; Li, Y.; Xing, X.H.
Controllable production of low molecular weight heparins by combinations of heparinase I/II/III
Carbohydr. Polym.
101
484-492
2014
Pedobacter heparinus
Manually annotated by BRENDA team
Cordula, C.R.; Lima, M.A.; Shinjo, S.K.; Gesteira, T.F.; Pol-Fachin, L.; Coulson-Thomas, V.J.; Verli, H.; Yates, E.A.; Rudd, T.R.; Pinhal, M.A.; Toma, L.; Dietrich, C.P.; Nader, H.B.; Tersariol, I.L.
On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+
Mol. Biosyst.
10
54-64
2014
Pedobacter heparinus, Pedobacter heparinus ATCC 13125
Manually annotated by BRENDA team
Huang, J.; Cao, L.; Guo, W.; Yuan, R.; Jia, Z.; Huang, K.
Enhanced soluble expression of recombinant Flavobacterium heparinum heparinase I in Escherichia coli by fusing it with various soluble partners
Protein Expr. Purif.
83
169-176
2012
Pedobacter heparinus
Manually annotated by BRENDA team
Balasubramaniam, K.; Sharma, K.; Rani, A.; Rajulapati, V.; Goyal, A.
Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltans
Ann. Microbiol.
68
409-418
2018
Pseudopedobacter saltans (F0S8P2), Pseudopedobacter saltans DSM 12145 (F0S8P2)
-
Manually annotated by BRENDA team
Yu, P.; Jia, T.; Chen, Y.; Wu, Y.; Zhang, Y.
Improving the activity of heparinase I by the directed evolution, its enzymatic properties and optimal conditions for heparin degrading by recombinant cells
Biochem. Eng. J.
114
237-243
2016
Pedobacter heparinus (B3U3D9)
-
Manually annotated by BRENDA team
Yang, M.; Chen, J.; Zhou, H.; Li, W.; Wang, Y.; Li, J.; Zhang, C.; Zhou, C.; Yu, C.
Polycation-induced benzoperylene probe excimer formation and the ratiometric detection of heparin and heparinase
Biosens. Bioelectron.
75
404-410
2016
Pedobacter heparinus
Manually annotated by BRENDA team
Yang, B.; Zhang, C.; Wang, C.; Zhou, H.; Li, Z.; Song, Y.; Zhang, T.; Luo, X.
Soluble expression and purification of heparinase I in Escherichia coli using a hexahistidine-tagged small ubiquitin-like modifier as a fusion partner
Biotechnol. Biotechnol. Equip.
31
1040-1045
2017
Pedobacter heparinus
-
Manually annotated by BRENDA team
Dzvova, N.; Colmer-Hamood, J.A.; Griswold, J.A.; Hamood, A.N.
Isolation and characterization of HepP a virulence-related Pseudomonas aeruginosa heparinase
BMC Microbiol.
17
233
2017
Pseudomonas aeruginosa (A0A0H2ZDM9), Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14 (A0A0H2ZDM9)
Manually annotated by BRENDA team
Bohlmann, L.; Chang, C.W.; Beacham, I.; von Itzstein, M.
Exploring bacterial heparinase II activities with defined substrates
ChemBioChem
16
1205-1211
2015
Pedobacter heparinus, Bacteroides eggerthii (E5X103), Bacteroides eggerthii, Bacteroides eggerthii 1_2_48FAA (E5X103)
Manually annotated by BRENDA team
Sefrioui, D.; Beaussire, L.; Clatot, F.; Delacour, J.; Perdrix, A.; Frebourg, T.; Michel, P.; Di Fiore, F.; Sarafan-Vasseur, N.
Heparinase enables reliable quantification of circulating tumor DNA from heparinized plasma samples by droplet digital PCR
Clin. Chim. Acta
472
75-79
2017
Bacteroides eggerthii
Manually annotated by BRENDA team
Dzvova, N.; Colmer-Hamood, J.A.; Griswold, J.A.; Hamood, A.N.
Heparinase is essential for Pseudomonas aeruginosa virulence during thermal injury and infection
Infect. Immun.
86
e00755
2018
Pseudomonas aeruginosa (A0A0H2ZDM9), Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14 (A0A0H2ZDM9)
Manually annotated by BRENDA team
Xu, S.; Qiu, M.; Zhang, X.; Chen, J.
Expression and characterization of an enhanced recombinant heparinase I with chitin binding domain
Int. J. Biol. Macromol.
105
1250-1258
2017
Pedobacter heparinus (B3U3D9)
Manually annotated by BRENDA team