Information on EC 4.2.2.22 - pectate trisaccharide-lyase

for references in articles please use BRENDA:EC4.2.2.22
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.2.22
-
RECOMMENDED NAME
GeneOntology No.
pectate trisaccharide-lyase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
eliminative cleavage of unsaturated trigalacturonate as the major product from the reducing end of polygalacturonic acid/pectate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-galacturonan reducing-end-trisaccharide-lyase
Differs in specificity from EC 4.2.2.9, pectate disaccharide-lyase, as the predominant action is removal of a trisaccharide rather than a disaccharide from the reducing end. Disaccharides and tetrasaccharides may also be removed [2].
CAS REGISTRY NUMBER
COMMENTARY hide
9015-75-2
cf. EC 4.2.2.2
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
ATCC 35296
SwissProt
Manually annotated by BRENDA team
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
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-
-
Manually annotated by BRENDA team
subspecies Yersinia enterocolitica 8081
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
PelA knockout mutants do not show significant differences in virulence compared to the wild-type strains during infection of Triticum aestivum plants
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pectin
?
show the reaction diagram
pectin
unsaturated (1,4-alpha-D-galacturonide)3
show the reaction diagram
pectin
unsaturated trigalacturonate
show the reaction diagram
polygalacturonic acid
4,5-unsaturated polygalacturonic acid
show the reaction diagram
polygalacturonic acid
trigalacturonic acid + digalacturonic acid
show the reaction diagram
polygalacturonic acid
unsaturated (1,4-alpha-D-galacturonide)3 + unsaturated (1,4-alpha-D-galacturonide)2
show the reaction diagram
-
-
-
-
?
polygalacturonic acid
unsaturated trigalacturonate
show the reaction diagram
rhamnogalacturonan
?
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pectin
?
show the reaction diagram
polygalacturonic acid
trigalacturonic acid + digalacturonic acid
show the reaction diagram
Q9AQF3
pectate lyase A is a subunit of the extracellular multienzyme complex, called the cellulosome, which is involved in plant cell wall degradation
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
optimal activity at 0.05-0.5 mM
Fe2+
-
stimulating
Fe3+
-
stimulating
Mg2+
-
stimulating
Mn2+
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stimulating
NaCl
-
highest activity at 200 mM
additional information
-
utilizes a metal atom other than calcium for catalysis
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
1 mM reduces activity to less than that without addition of CaCl2
Cu2+
-
inhibitory
EGTA
-
1 mM, complete inhibition
polygalacturonic acid
recombinant fusion protein containing a C-terminal His-tag is inhibited by excess substrate
Zn2+
-
inhibitory
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
polygalacturonic acid
-
-
additional information
additional information
Km-value for polygalacturonic acid is 0.56 g/l, recombinant fusion protein containing a C-terminal His-tag
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Ki-value for polygalacturonic acid is 1.08 g/l
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.37
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90% esterified pectin
6.38
-
60% esterified pectin
57.3
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polygalacturonic acid
63.7
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30% esterified pectin
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
pH 7.0: less than 30% of maximal activity, pH 8.0: optimum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
recombinant fusion protein containing a C-terminal His-tag
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
more than 50% of maximal activity at 40°C and at 60°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33451
x * 33451, calculated from sequence
36000
x * 36000, SDS-PAGE
42000
x * 42000, SDS-PAGE
152000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, in 30% polyethylene glycol 1500 and 0.1 M sodium citrate, pH 5.0
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
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at 50°C
682998
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95
-
half-life: 110 min
100
-
2 min, 50% loss of activity
103
-
apparent melting temperature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ stabilizes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the recombinant protein using Ni2+-nitrilotriacetateagarose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli as a recombinant fusion protein containing a C-terminal His-tag
overexpression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
construction of PelA knockout mutants
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
industry