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The expected taxonomic range for this enzyme is: Eubacterium sp.
Synonyms
16-dehydroprogesterone reductase,
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16-dehydroprogesterone reductase
16alpha-dehydroxylase
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16alpha-Hydroxyprogesterone dehydroxylase
Dehydroxylase, hydroxy steroid 16alpha
dehydroxylase, hydroxy steroid 16alpha-
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EC 4.2.1.86
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formerly
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Hydroxyprogesterone dehydroxylase
reductase, 16-dehydroprogesterone
16-dehydroprogesterone reductase
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16-dehydroprogesterone reductase
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16alpha-Hydroxyprogesterone dehydroxylase
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16alpha-Hydroxyprogesterone dehydroxylase
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16alpha-Hydroxyprogesterone dehydroxylase
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Dehydroxylase, hydroxy steroid 16alpha
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Dehydroxylase, hydroxy steroid 16alpha
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Dehydroxylase, hydroxy steroid 16alpha
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Hydroxyprogesterone dehydroxylase
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Hydroxyprogesterone dehydroxylase
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Hydroxyprogesterone dehydroxylase
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reductase, 16-dehydroprogesterone
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reductase, 16-dehydroprogesterone
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16alpha-Hydroxyprogesterone = 16,17-didehydroprogesterone + H2O
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C-O bond cleavage by elimination of water
C-O bond cleavage by elimination of water
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C-O bond cleavage by elimination of water
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C-O bond cleavage by elimination of water
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16alpha-hydroxyprogesterone hydro-lyase (16,17-didehydroprogesterone-forming)
16alpha-Hydroxypregnenolone is also a substrate.
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16-dehydroprogesterone + H2O
16alpha-hydroxyprogesterone
16alpha-Hydroxypregnanolone
Iso-pregnanolone
16alpha-hydroxypregnenolone
16-dehydropregnenolone + H2O
16alpha-Hydroxypregnenolone
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16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
16alpha-hydroxyprogesterone
16dehydroprogesterone + H2O
16alpha-hydroxyprogesterone
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16alpha-hydroxyprogesterone
DELTA16-progesterone + H2O
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in the hydration direction the enzyme is only 15% as active as in the dehdyration direction
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r
16-dehydroprogesterone + H2O
16alpha-hydroxyprogesterone
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pyruvate or sodium dithionite serve as electron donors, both reactions require methylviologen as electron carrier
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16-dehydroprogesterone + H2O
16alpha-hydroxyprogesterone
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pyruvate or sodium dithionite serve as electron donors, both reactions require methylviologen as electron carrier
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?
16alpha-Hydroxypregnanolone
Iso-pregnanolone
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16alpha-Hydroxypregnanolone
Iso-pregnanolone
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16alpha-hydroxypregnenolone
16-dehydropregnenolone + H2O
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16alpha-hydroxypregnenolone
16-dehydropregnenolone + H2O
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16alpha-Hydroxypregnenolone
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16alpha-Hydroxypregnenolone
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activity is slight less than with 16alpha-hydroxyprogesterone
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16alpha-Hydroxypregnenolone
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activity is slight less than with 16alpha-hydroxyprogesterone
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16alpha-Hydroxypregnenolone
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16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
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16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
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r
16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
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pyruvate or Na dithionite serve as electron donor and both reactions require methyl viologen as an electron carrier
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16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
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16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
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r
16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
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pyruvate or Na dithionite serve as electron donor and both reactions require methyl viologen as an electron carrier
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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r
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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in the reverse reaction the enzyme is only 15% as active as in the forward reaction
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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r
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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in the reverse reaction the enzyme is only 15% as active as in the forward reaction
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16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
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16alpha-hydroxyprogesterone
16dehydroprogesterone + H2O
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16alpha-hydroxyprogesterone
16dehydroprogesterone + H2O
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16alpha-hydroxyprogesterone
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16alpha-hydroxyprogesterone
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inducible enzyme
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16alpha-hydroxyprogesterone
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16alpha-hydroxyprogesterone
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inducible enzyme
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DELTA16-Progesterone
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DELTA16-Progesterone
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16alpha-hydroxyprogesterone
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16alpha-hydroxyprogesterone
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inducible enzyme
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16alpha-hydroxyprogesterone
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inducible enzyme
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16-Ketoprogesterone
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competitive with respect to 16alpha-hydroxypregnenolone and 16alpha-hydroxyprogesterone
16alpha-hydroxypregnenolone
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substrate inhibition at high concentrations
cetyltrimethylammonium bromide
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no inhibition by NaBr
HgCl2
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0.1 mM, 87% inhibition
PCMB
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0.5 mM, 50% inhibition
Rifampin
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i.e. rifampicin
additional information
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not inhibited by inhibitors of electron transport reactions or metal chelators
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Tris-HCl
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at pH above 7
Tris-HCl
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at pH above 7.0
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methanol
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active at 15-30% v/v methanol with an optimal concentration of 25%
SDS
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0.1%, stimulates
SDS
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0.05%, about 7fold stimulation
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0.25
16alpha-hydroxypregnenolone
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0.52 - 0.56
16alpha-hydroxyprogesterone
0.52
16alpha-hydroxyprogesterone
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0.52
16alpha-hydroxyprogesterone
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pH 6.8
0.56
16alpha-hydroxyprogesterone
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0.56
16alpha-hydroxyprogesterone
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pH 6.8, 37°C
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0.08 - 0.18
16-Ketoprogesterone
0.08
16-Ketoprogesterone
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pH 6.8, reaction with 16alpha-hydroxyprogesterone
0.18
16-Ketoprogesterone
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pH 6.8, reaction with 16alpha-hydroxypregnenolone
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5 - 8.5
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activity is unaffected by pH in potassium phosphate buffer over the range pH 5.0-8.5
5.8 - 8
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the enzyme exhibits a broad pH range without a distinct pH-optimum
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5 - 8.5
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enzyme activity is not affected over this range in potassium phosphate buffer, but is inhibited by Tris-HCl buffer at pH above 7.0
5.8 - 8
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broad pH-range without a distinct pH-optimum
5.8 - 8
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the enzyme exhibits a broad pH range without a distinct pH-optimum. Above pH 8.5 the activity decreases and is undetectable at pH 10.0
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35 - 50
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35°C: about 65% of maximal activity, 50°C: about 40% of maximal activity
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brenda
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brenda
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40% activity in cytoplasmic, 20% in membrane fractions of crude cell extract
brenda
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40% of the activity
brenda
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40% activity in cytoplasmic, 20% in membrane fractions of crude cell extract
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brenda
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40% of the activity
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brenda
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20% of the activity
brenda
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40% activity in cytoplasmic, 20% in membrane fractions of crude cell extract
brenda
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40% activity in cytoplasmic, 20% in membrane fractions of crude cell extract
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brenda
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20% of the activity
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brenda
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181000
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tetrameric form, gel filtration
181000
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active in two molecular weight forms, gel filtration
326000
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octameric form, gel filtration
326000
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active in two molecular weight forms, gel filtration
42400
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4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE
42400
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8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE
42400
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x * 42400, SDS-PAGE
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?
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x * 42400, SDS-PAGE
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x * 42400, SDS-PAGE
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octamer
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8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE
octamer
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8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE
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tetramer
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4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE
tetramer
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4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE
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65
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6 min, 70% loss of activity
65
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10 min, 70% inactivation
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Methanol
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high tolerance to
Methanol
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high tolerance to
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Glass, T.L.; Burley, C.Z.
Biotransformation of 16-dehydroprogesterone by the intestinal anaerobic bacterium, Eubacterium sp. 144
J. Steroid Biochem.
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65-72
1984
Eubacterium sp., Eubacterium sp. 144
brenda
Winter, J.; O'Rourke, S.; Bokkenheuser, V.D.; Hylemon, P.B.; Glass, T.L.
16alpha-Dehydration of corticoids by bacteria isolated from rat fecal flora
J. Steroid Biochem.
16
231-237
1982
Eubacterium sp., Eubacterium sp. 144
brenda
Glass, T.L.; Lamppa, R.S.; Hylemon, P.B.
Characterization of 16alpha-hydroxyprohgesterone dehydroxylase in cell extracts of the intestinal anaerobic bacterium Eubacterium sp. 144
Biochim. Biophys. Acta
792
59-64
1984
Eubacterium sp., Eubacterium sp. 144
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brenda
Glass, T.L.; Lamppa, R.S.
Purification and properties of 16alpha-hydroxyprogesterone dehydroxylase from Eubacterium sp. Strain 144
Biochim. Biophys. Acta
837
103-110
1985
Eubacterium sp., Eubacterium sp. 144
brenda
Watkins, W.E.; Glass, T.L.
Characteristics of 16-dehydroprogesterone reductase in cell extracts of the intestinal anaerobe, Eubacterium sp. strain 144
J. Steroid Biochem. Mol. Biol.
38
257-263
1991
Eubacterium sp., Eubacterium sp. 144
brenda
Glass, T.L.; Burley, C.Z.
Stimulation of 16-dehydroprogesterone and progesterone reductases of Eubacterium sp. strain 144 by hemin and hydrogen or pyruvate
Appl. Environ. Microbiol.
49
1146-1153
1985
Eubacterium sp., Eubacterium sp. 144
brenda
Watkins, W.E.; Glass, T.L.
Characteristics of 16-dehydroprogesterone reductase in cell extracts of the intestinal anaerobe, Eubacterium sp. strain 144
J. Steroid Biochem.
38
257-263
1991
Eubacterium sp., Eubacterium sp. 144
brenda
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4.2.1.98 16alpha-hydroxyprogesterone dehydratase
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