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Information on EC 4.2.1.85 - dimethylmaleate hydratase for references in articles please use BRENDA:EC4.2.1.85
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EC Tree
The expected taxonomic range for this enzyme is: Eubacterium barkeri
Synonyms
hydratase, dimethylmaleate,
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hydratase, dimethylmaleate
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(2R,3S)-2,3-dimethylmalate = dimethylmaleate + H2O
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C-O bond cleavage by elimination of water
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(2R,3S)-2,3-dimethylmalate hydro-lyase (dimethylmaleate-forming)
Requires Fe2+. Inhibited by oxygen.
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dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
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r
dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
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equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate
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dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
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additional information
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Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+
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5,5'-dithiobis(2-nitrobenzoate)
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1 mM: 97% inhibition
EDTA
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1-5 mM: 70% loss of activity
N-ethylmaleimide
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1 mM: 93% inhibition
additional information
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10 min, 25°C, 2 mM iodoacetamide or iodoacetate: no influence
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3.1
dimethylmaleate
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35°C, pH 7
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brenda
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unstable during purification, probably because of dissociation into inactive protein compounds
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oxygen-sensitive
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648458
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-20°C, 45% loss of activity within 1 day
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4°C, 85% loss of activity within 1 day
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liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0
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nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions
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Kollmann-Koch, A.; Eggerer, H.
Nicotinic acid metabolism. Dimethylmaleate hydratase
Hoppe-Seyler's Z. Physiol. Chem.
365
847-857
1984
Eubacterium barkeri
brenda
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