Information on EC 4.2.1.85 - dimethylmaleate hydratase

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The expected taxonomic range for this enzyme is: Eubacterium barkeri

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EC NUMBER
COMMENTARY hide
4.2.1.85
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RECOMMENDED NAME
GeneOntology No.
dimethylmaleate hydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2R,3S)-2,3-dimethylmalate = dimethylmaleate + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-O bond cleavage by elimination of water
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nicotinate degradation III
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Nicotinate and nicotinamide metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(2R,3S)-2,3-dimethylmalate hydro-lyase (dimethylmaleate-forming)
Requires Fe2+. Inhibited by oxygen.
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CAS REGISTRY NUMBER
COMMENTARY hide
93229-56-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
show the reaction diagram
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-
-
r
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
required
additional information
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Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
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1 mM: 97% inhibition
EDTA
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1-5 mM: 70% loss of activity
N-ethylmaleimide
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1 mM: 93% inhibition
additional information
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10 min, 25°C, 2 mM iodoacetamide or iodoacetate: no influence
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
dimethylmaleate
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35°C, pH 7
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
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broad
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
assay at
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
153000
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gel filtration
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable during purification, probably because of dissociation into inactive protein compounds
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive
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648458
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 45% loss of activity within 1 day
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4°C, 85% loss of activity within 1 day
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liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATCC 25849, partial
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Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions
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Show AA Sequence (404 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 4.2.1.85)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)