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Information on EC 4.2.1.70 - pseudouridylate synthase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q06244

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.70 pseudouridylate synthase
IUBMB Comments
The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo .
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Saccharomyces cerevisiae
UNIPROT: Q06244
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
pseudouridine synthase, p35 protein, pseudouridine synthase i, pseudouridylate synthase, psi55 synthase, pseudouridylate synthetase, pseudouridine-5'-phosphate glycosidase, trna pseudouridine 55 synthase, orp protein, psi55 trna pseudouridine synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16S pseudouridine 516 synthase
-
-
-
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16S pseudouridylate 516 synthase
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-
-
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5-ribosyluracil 5'-phosphate synthetase
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-
-
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Depressed growth-RATE protein DEG1
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-
-
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ORP protein
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-
-
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Osmoprotectant regulator of PLC
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-
-
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P35 protein
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-
-
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pseudouridine monophosphate synthetase
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-
-
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pseudouridine synthase
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-
-
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pseudouridine synthase I
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Pseudouridylate synthase
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-
-
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pseudouridylate synthetase
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-
-
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pseudouridylate synthetase I
-
-
-
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pseudouridylate synthetase II
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-
-
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pseudouridylic acid synthetase
-
-
-
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PSI55 synthase
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-
-
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psiMP synthetase
-
-
-
-
psisynthetase
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-
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psiUMP synthetase
-
-
-
-
PSUI
-
-
-
-
synthase, pseudouridylate
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-
-
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tRNA pseudouridine 55 synthase
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-
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uracil hydrolyase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
uracil hydro-lyase (adding D-ribose 5-phosphate; pseudouridine-5'-phosphate-forming)
The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9023-35-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNAIle
?
show the reaction diagram
-
-
-
-
?
tRNAVal
?
show the reaction diagram
-
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
essential for its native conformation and tRNA recognition
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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loss of activity within 5 min in the presence of 40 mM pseudouridine synthetase Pus1
thiol reagents
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00074
tRNAIle
-
Pus1, intronless yeast tRNAIle
0.00042 - 0.0015
tRNAVal
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00833
tRNAIle
-
substrate from yeast, intronless, limiting
0.00667 - 0.00833
tRNAVal
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62100
-
Pus1p, calculation from genomic sequence
63200
-
1 * 63200, MALDI mass spectrometry
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 63200, MALDI mass spectrometry
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Pus1p, expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matsushita, T.; Davis, F.F.
Studies on pseudouridylic acid synthetase from various sources
Biochim. Biophys. Acta
238
165-173
1971
Agrobacterium tumefaciens, Bos taurus, Brassica oleracea, Saccharomyces cerevisiae, Lolium perenne, Nicotiana glauca, Spinacia oleracea, Agrobacterium tumefaciens B6-6
Manually annotated by BRENDA team
Motorin, Y.; Keith, G.; Simon, C; Foiret, D.; Simos, G.; Hurt, E.; Grosjean, H.
The yeast tRNA: pseudouridine synthase Pus1p displays a multisite substrate specificity
RNA
4
856-869
1998
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Arluison, V.; Hountondji, C.; Robert, B.; Grosjean, H.
Transfer RNA-Pseudouridine Synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition
Biochemistry
37
7268-7276
1998
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Massenet, S.; Motorin, Y.; Lafontaine, D.L.J.; Hurt, E.; Grosjean, H.; Branlant, C.
Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase Pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA
Mol. Cell. Biol.
19
2142-2154
1999
Saccharomyces cerevisiae, Saccharomyces cerevisiae FL100
Manually annotated by BRENDA team
Arluison, V.; Buckle, M.; Grosjean, H.
Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA
J. Mol. Biol.
289
491-502
1999
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Ansmant, I.; Massenet, S.; Grosjean, H.; Motorin, Y.; Branlant, C.
Identification of the Saccharomyces cerevisiae RNA:pseudouridine synthase responsible for formation of psi2819 in 21S mitochondrial ribosomal RNA
Nucleic Acids Res.
28
1941-1946
2000
Saccharomyces cerevisiae (Q06244)
Manually annotated by BRENDA team