Information on EC 4.2.1.70 - pseudouridylate synthase

for references in articles please use BRENDA:EC4.2.1.70
Word Map on EC 4.2.1.70
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.2.1.70
-
RECOMMENDED NAME
GeneOntology No.
pseudouridylate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
elimination
-
of H2O, C-O bond cleavage
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pseudouridine degradation
-
-
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
uracil hydro-lyase (adding D-ribose 5-phosphate; pseudouridine-5'-phosphate-forming)
The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9023-35-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
15955 (ATCC)
-
-
Manually annotated by BRENDA team
B6-6 (ATCC)
-
-
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
var. italica
-
-
Manually annotated by BRENDA team
pseudouridine synthase 1
-
-
Manually annotated by BRENDA team
pseudouridine synthase 1
-
-
Manually annotated by BRENDA team
EB146 (leuK16)
-
-
Manually annotated by BRENDA team
RsuA
-
-
Manually annotated by BRENDA team
rye grass
-
-
Manually annotated by BRENDA team
normal and with Agrobacterium tumefaciens transformed tissue
-
-
Manually annotated by BRENDA team
FL100 (ATCC)
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
TruB
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-thiouracil + D-ribose 5-phosphate
?
show the reaction diagram
-
-
-
-
-
isocytosine + D-ribose 5-phosphate
?
show the reaction diagram
-
-
-
-
-
pseudouridine 5' phosphate + H2O
?
show the reaction diagram
pseudouridine 5'-phosphate + H2O
uracil + D-ribose 5-phosphate
show the reaction diagram
tRNAIle
?
show the reaction diagram
-
-
-
-
-
tRNAVal
?
show the reaction diagram
-
-
-
-
-
uracil + D-ribose 5-phosphate
?
show the reaction diagram
-
biosynthetic pathway for pseudouridylic acid and pseudouridine
-
-
-
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
show the reaction diagram
uracil + deoxyribose 5-phosphate
deoxypseudouridine 5'-phosphate + H2O
show the reaction diagram
uracil + ribose 5-phosphate
5-ribosyluracil 5'-monophosphate + H2O
show the reaction diagram
-
r
-
-
uridinyl-tRNA
pseudouridinyl-tRNA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pseudouridine 5' phosphate + H2O
?
show the reaction diagram
uracil + D-ribose 5-phosphate
?
show the reaction diagram
-
biosynthetic pathway for pseudouridylic acid and pseudouridine
-
-
-
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
show the reaction diagram
uridinyl-tRNA
pseudouridinyl-tRNA
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
high activity
Fe2+
high activity
Mg2+
-
required, broad maximal activity between 0.01 M and 0.05 M
Mn2+
high activity
Zn2+
-
essential for its native conformation and tRNA recognition
additional information
YeiN is dependent on divalent cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
loss of activity within 5 min in the presence of 40 mM pseudouridine synthetase Pus1
1-(Tetrahydro-2-furanyl)-5-fluorouracil
-
i.e. ftorafur
5-fluorouracil
-
-
iodoacetamide
-
150 mM, in about 5 min all activity is lost
p-chloromercuribenzoate
thiol reagents
-
-
tRNA
-
wild-type RNA, 50% inhibition at a concentration of 0.005 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
pseudouridine 5'-phosphate
pH 7.1, 30°C
0.024 - 0.84
ribose 5-phosphate
0.00074
tRNAIle
-
Pus1, intronless yeast tRNAIle
0.00042 - 0.0015
tRNAVal
0.071 - 0.67
Uracil
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00833
tRNAIle
-
substrate from yeast, intronless, limiting
0.00667 - 0.00833
tRNAVal
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
-
pH 7.0 about 50% of maximal activity, pH 9.5 about 80% of maximal activity
7.5 - 10
-
pH 7.5 about 40% of maximal activity, pH 10 about 20% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
-
about 40% of maximal activity at 10°C and about 10% of maximal activity at 50°C
24 - 37
-
about 50% of maximal activity at 25°C and about 30% of maximal activity at 37°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Escherichia coli (strain K12);
P45124
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd);
Q8Q0M2
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88);
P9WHP7
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
pseudouridine synthase I, calculation from genomic sequence
46610
-
pus1p, calculation from sequence of cDNA
50000
-
2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization
62100
-
Pus1p, calculation from genomic sequence
63200
-
1 * 63200, MALDI mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pseudouridine synthase RluC fragment
-
TruB, two substrate-free forms, sitting-drop vapor-diffusion method
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
no activity at or below
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity retained after freezing and thawing
-
buffers containing 20% glycerol stabilize the enzyme
-
enzyme retains its activity during dialysis
-
mercaptoethanol is very effective in restoring the original activity of aged enzyme preparations
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 1 year without loss of activity
-
4°C, storage for 40 days, preincubation with 15 mM mercaptoethanol restores the original activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+ Sepharose column chromatography and Superdex 75 gel filtration
-
partial
pseudouridylate synthetase I, partial
-
recombinant His-tagged enzyme from Escherichia coli by affinity chromatography
recombinant TruB
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expression as His-tagged enzyme in Escherichia coli
Pus1p, expression in Escherichia coli
RluC, expression in Escherichia coli
-
Rv2793c gene, expression in Escherichia coli
-
Show AA Sequence (5208 entries)
Longer loading times are possible. Please use the Sequence Search for a specific query.