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Information on EC 4.2.1.70 - pseudouridylate synthase
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4.2.1.70 pseudouridylate synthaseIUBMB Comments
The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo .
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Word Map
- 4.2.1.70
-
pseudouridylation
-
baculovirus
- ribonucleoproteins
- congenita
-
dyskeratosis
-
dyskerin
-
rna-guided
-
snornps
-
rna-modifying
-
sideroblastic
- roscovitine
-
archaeosine
- gar1
- nop10
- his
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
pseudouridine synthase, p35 protein, pseudouridine synthase i, pseudouridylate synthase, psi55 synthase, pseudouridylate synthetase, pseudouridine-5'-phosphate glycosidase, trna pseudouridine 55 synthase, orp protein, psi55 trna pseudouridine synthase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
16S pseudouridine 516 synthase
-
-
-
-
16S pseudouridylate 516 synthase
-
-
-
-
5-ribosyluracil 5'-phosphate synthetase
-
-
-
-
Depressed growth-RATE protein DEG1
-
-
-
-
ORP protein
-
-
-
-
Osmoprotectant regulator of PLC
-
-
-
-
P35 protein
-
-
-
-
pseudouridine monophosphate synthetase
-
-
-
-
pseudouridine synthase
pseudouridine synthase I
-
-
-
-
pseudouridine-5'-phosphate glycosidase
Pseudouridylate synthase
-
-
-
-
pseudouridylate synthetase
-
-
-
-
pseudouridylate synthetase I
-
-
-
-
pseudouridylate synthetase II
-
-
-
-
pseudouridylic acid synthetase
-
-
-
-
PSI55 synthase
-
-
-
-
psi55 tRNA pseudouridine synthase
psiMP synthetase
-
-
-
-
psisynthetase
-
-
-
-
psiUMP synthetase
-
-
-
-
PSUI
-
-
-
-
PUS
synthase, pseudouridylate
-
-
-
-
tRNA pseudouridine 55 synthase
-
-
-
-
TruB
uracil hydrolyase
-
-
-
-
YeiN
additional information
pseudouridine synthase
-
-
-
-
additional information
-
pseudouridine synthases are classified into the sequence families TruB, TruA, RsuA, RluA and TruD
additional information
-
pseudouridine synthases are classified into the sequence families TruB, TruA, RsuA, RluA and TruD
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
addition
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SYSTEMATIC NAME
IUBMB Comments
uracil hydro-lyase (adding D-ribose 5-phosphate; pseudouridine-5'-phosphate-forming)
The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo [5].
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CAS REGISTRY NUMBER
COMMENTARY
9023-35-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
uracil + D-ribose 5-phosphate
?
-
biosynthetic pathway for pseudouridylic acid and pseudouridine
-
-
?
uracil + ribose 5-phosphate
5-ribosyluracil 5'-monophosphate + H2O
-
r
-
?
pseudouridine 5' phosphate + H2O
?
-
a catabolite-repressible enzyme. Enzyme is involved in the utilization in vivo of exogenous pseudouridine
-
-
?
pseudouridine 5' phosphate + H2O
?
-
growth on pseudouridine as sole pyrimidine source
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
r
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
r
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
in presence of sodium arsenate
in presence of sodium arsenate
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
r
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
r
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
r
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
the enzyme is involved in the metabolism of pseudouridine in uropathogenic Escherichia coli
-
-
r
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
r
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
the association of IndA with pseudouridine kinase indicates that it serves physiologically to metabolize pseudouridine 5'-phosphate rather than to form it
-
-
r
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
IndA catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner by cleavage of the glycosidic C-C bond of pseudouridine
-
-
r
uracil + deoxyribose 5-phosphate
deoxypseudouridine 5'-phosphate + H2O
-
r
dpsiMP
?
uracil + deoxyribose 5-phosphate
deoxypseudouridine 5'-phosphate + H2O
-
enzyme utilizes deoxyribose 5-phosphate at 30-40% of the rate with ribose 5-phosphate
-
?
uracil + deoxyribose 5-phosphate
deoxypseudouridine 5'-phosphate + H2O
-
enzyme utilizes deoxyribose 5-phosphate at 30-40% of the rate with ribose 5-phosphate
-
?
uracil + deoxyribose 5-phosphate
deoxypseudouridine 5'-phosphate + H2O
-
-
-
-
?
uridinyl-tRNA
pseudouridinyl-tRNA
-
TruB is specific for the nearly universal psi55 in tRNA, mechanism of tRNA recognition, protein dynamics, base flipping and modification play a role in tRNA recognition, TruB structure, 2 domains: the large N-terminal catalytic domain and the short C-terminal RNA-binding domain
-
?
uridinyl-tRNA
pseudouridinyl-tRNA
-
TruB is specific for the nearly universal psi55 in tRNA
-
?
uridinyl-tRNA
pseudouridinyl-tRNA
-
TruB is specific for the nearly universal psi55 in tRNA, mechanism of tRNA recognition, protein dynamics, base flipping and modification play a role in tRNA recognition, TruB structure, 2 domains: the large N-terminal catalytic domain and the short C-terminal RNA-binding domain
-
?
uridinyl-tRNA
pseudouridinyl-tRNA
-
TruB is specific for the nearly universal psi55 in tRNA
-
?
additional information
?
-
-
mutation of the genlocus hisT causes lack of pseudouridylate synthetase activity. The defect in this enzyme leads to the absence of pseudouridines in the anticodon loop of several tRNA species
-
-
?
additional information
?
-
-
pseudouridine synthase I activity is missing in extracts of strain EB146 (leuK16)
-
-
?
additional information
?
-
-
genetic link between tRNA pseudouridine modification and vitamin B6 biosynthesis
-
-
?
additional information
?
-
measuring the production of ribose 5-phosphate with a ribose-5-phosphate isomerase/ribulose-5-phosphate reductase-coupled assay, no activity with ribonucleotide monophosphates AMP, UMP, GMP, and CMP, overview
-
-
?
additional information
?
-
-
measuring the production of ribose 5-phosphate with a ribose-5-phosphate isomerase/ribulose-5-phosphate reductase-coupled assay, no activity with ribonucleotide monophosphates AMP, UMP, GMP, and CMP, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes pseudouridylation at position 55 in the T-pseudouridine-C arm of all elongator tRNAs
-
-
?
additional information
?
-
-
pseudouridine synthase I activity is missing in extracts of strain EB146 (leuK16)
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p displays a multisite substrate specificity
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p displays a multisite substrate specificity
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA
-
-
?
additional information
?
-
-
pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species
-
-
?
additional information
?
-
-
pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species
-
-
?
additional information
?
-
-
inaccurate protein synthesis in a mutant of Salmonella typhimurium defective in transfer RNA pseudouridylation
-
-
?
additional information
?
-
-
pseudouridylate synthetase II is responsible for the biosynthesis of pseudouridine in the TpsiCG loop of every tRNA molecule
-
-
?
additional information
?
-
-
inaccurate protein synthesis in a mutant of Salmonella typhimurium defective in transfer RNA pseudouridylation
-
-
?
additional information
?
-
-
activity measurement in a two-step assay
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
uracil + D-ribose 5-phosphate
?
-
biosynthetic pathway for pseudouridylic acid and pseudouridine
-
-
?
pseudouridine 5' phosphate + H2O
?
-
a catabolite-repressible enzyme. Enzyme is involved in the utilization in vivo of exogenous pseudouridine
-
-
?
pseudouridine 5' phosphate + H2O
?
-
growth on pseudouridine as sole pyrimidine source
-
-
?
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
the enzyme is involved in the metabolism of pseudouridine in uropathogenic Escherichia coli
-
-
r
uracil + D-ribose 5-phosphate
pseudouridine 5'-phosphate + H2O
-
the association of IndA with pseudouridine kinase indicates that it serves physiologically to metabolize pseudouridine 5'-phosphate rather than to form it
-
-
r
uridinyl-tRNA
pseudouridinyl-tRNA
-
TruB is specific for the nearly universal psi55 in tRNA
-
?
uridinyl-tRNA
pseudouridinyl-tRNA
-
TruB is specific for the nearly universal psi55 in tRNA
-
?
additional information
?
-
-
mutation of the genlocus hisT causes lack of pseudouridylate synthetase activity. The defect in this enzyme leads to the absence of pseudouridines in the anticodon loop of several tRNA species
-
-
?
additional information
?
-
-
pseudouridine synthase I activity is missing in extracts of strain EB146 (leuK16)
-
-
?
additional information
?
-
-
genetic link between tRNA pseudouridine modification and vitamin B6 biosynthesis
-
-
?
additional information
?
-
-
the enzyme catalyzes pseudouridylation at position 55 in the T-pseudouridine-C arm of all elongator tRNAs
-
-
?
additional information
?
-
-
pseudouridine synthase I activity is missing in extracts of strain EB146 (leuK16)
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p displays a multisite substrate specificity
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p displays a multisite substrate specificity
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA
-
-
?
additional information
?
-
-
pseudouridine synthase Pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA
-
-
?
additional information
?
-
-
pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species
-
-
?
additional information
?
-
-
pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species
-
-
?
additional information
?
-
-
inaccurate protein synthesis in a mutant of Salmonella typhimurium defective in transfer RNA pseudouridylation
-
-
?
additional information
?
-
-
pseudouridylate synthetase II is responsible for the biosynthesis of pseudouridine in the TpsiCG loop of every tRNA molecule
-
-
?
additional information
?
-
-
inaccurate protein synthesis in a mutant of Salmonella typhimurium defective in transfer RNA pseudouridylation
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
loss of activity within 5 min in the presence of 40 mM pseudouridine synthetase Pus1
iodoacetamide
-
150 mM, in about 5 min all activity is lost
tRNA
-
wild-type RNA, 50% inhibition at a concentration of 0.005 mM
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Acidosis, Lactic
Nonsense mutation in pseudouridylate synthase 1 (PUS1) in two brothers affected by myopathy, lactic acidosis and sideroblastic anaemia (MLASA).
African Swine Fever
[Comparison of the antigenicity of African swine fever virus p35 protein as diagnostic antigen].
Anemia, Sideroblastic
Nonsense mutation in pseudouridylate synthase 1 (PUS1) in two brothers affected by myopathy, lactic acidosis and sideroblastic anaemia (MLASA).
Anemia, Sideroblastic
Unusual clinical expression and long survival of a pseudouridylate synthase (PUS1) mutation into adulthood.
Bone Marrow Failure Disorders
PATHOGENIC NAP57 MUTATIONS DECREASE RIBONUCLEOPROTEIN ASSEMBLY IN DYSKERATOSIS CONGENITA.
Brain Ischemia
Calpain-induced proteolysis after transient global cerebral ischemia and ischemic tolerance in a rat model.
Carcinoid Tumor
Achaete-scute homologue-1 (ASH1) stimulates migration of lung cancer cells through Cdk5/p35 pathway.
Carcinoma, Hepatocellular
Oxidatively modified protein-disulfide isomerase-associated 3 promotes dyskerin pseudouridine synthase 1-mediated malignancy and survival of hepatocellular carcinoma cells.
Carcinoma, Squamous Cell
p35 protein as a prognostic factor of squamous cell carcinoma of the oesophagus.
Cerebral Infarction
Effect of baculovirus P35 protein on apoptosis in brain tissue of rats with acute cerebral infarction.
Colonic Neoplasms
Pseudouridylate Synthase 7 Promotes Cell Proliferation and Invasion in Colon Cancer Through Activating PI3K/AKT/mTOR Signaling Pathway.
Cowpox
Role and regulation of activation of caspases in cisplatin-induced injury to renal tubular epithelial cells.
Dyskeratosis Congenita
Development of metachronous rectal cancers in a young man with dyskeratosis congenita: a case report.
Dyskeratosis Congenita
Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues.
Dyskeratosis Congenita
Landscape of the complete RNA chemical modifications in the human 80S ribosome.
Dyskeratosis Congenita
Linking pseudouridine synthases to growth, development and cell competition.
Dyskeratosis Congenita
Loss of function of the tumor suppressor DKC1 perturbs p27 translation control and contributes to pituitary tumorigenesis.
Dyskeratosis Congenita
PATHOGENIC NAP57 MUTATIONS DECREASE RIBONUCLEOPROTEIN ASSEMBLY IN DYSKERATOSIS CONGENITA.
Dyskeratosis Congenita
The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1.
Ectromelia
[Changes in antigenic properties of the p35 protein of vaccinia virus in various protein fractions of the virion with the use of monospecific antisera]
Genetic Diseases, Inborn
Landscape of the complete RNA chemical modifications in the human 80S ribosome.
Hepatitis C
Immunohistochemical study of syndecan-1 down-regulation and the expression of P35 protein in oral lichen planus: a clinicopathologic correlation with hepatitis C infection in the Egyptian population.
Infections
Immunohistochemical study of syndecan-1 down-regulation and the expression of P35 protein in oral lichen planus: a clinicopathologic correlation with hepatitis C infection in the Egyptian population.
Infections
In vivo clearance of an intracellular bacterium, Francisella tularensis LVS, is dependent on the p40 subunit of interleukin-12 (IL-12) but not on IL-12 p70.
Infections
Stable cell lines expressing baculovirus P35: resistance to apoptosis and nutrient stress, and increased glycoprotein secretion.
Intellectual Disability
Biochemical insight into pseudouridine synthase 7 (PUS7) as a novel interactor of sirtuin, SIRT1.
Leukemia
Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B).
Lichen Planus, Oral
Immunohistochemical study of syndecan-1 down-regulation and the expression of P35 protein in oral lichen planus: a clinicopathologic correlation with hepatitis C infection in the Egyptian population.
Microcephaly
Unusual clinical expression and long survival of a pseudouridylate synthase (PUS1) mutation into adulthood.
Muscular Diseases
Nonsense mutation in pseudouridylate synthase 1 (PUS1) in two brothers affected by myopathy, lactic acidosis and sideroblastic anaemia (MLASA).
Neoplasms
Inhibitory effects of Rhenium-188-labeled Herceptin on prostate cancer cell growth: A possible radioimmunotherapy to prostate carcinoma.
Neoplasms
Loss of Drosophila pseudouridine synthase triggers apoptosis-induced proliferation and promotes cell-nonautonomous EMT.
Neoplasms
Loss of function of the tumor suppressor DKC1 perturbs p27 translation control and contributes to pituitary tumorigenesis.
Neoplasms
Oxidatively modified protein-disulfide isomerase-associated 3 promotes dyskerin pseudouridine synthase 1-mediated malignancy and survival of hepatocellular carcinoma cells.
Neoplasms
The baculovirus p35 protein inhibits Fas- and tumor necrosis factor-induced apoptosis.
Persistent Infection
mRNA pseudouridylation affects RNA metabolism in the parasite Toxoplasma gondii.
Sarcoma
Altered expression and distribution of the cytoskeletal-associated p35 protein in NIH 3T3 cells transformed with the Harvey sarcoma virus v-ras oncogene.
Starvation
Functional Disruption of a Chloroplast Pseudouridine Synthase Desensitizes Arabidopsis Plants to Phosphate Starvation.
Tuberculosis
Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft.
Vaccinia
[Changes in antigenic properties of the p35 protein of vaccinia virus in various protein fractions of the virion with the use of monospecific antisera]
Vaccinia
[Identification of the gene for the immunodominant p35 protein from vaccinia virus]
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0015
tRNAVal
-
Pus1, yeast tRNAVal, in the presence of 5 mM EDTA and in the absence of Mg2+
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.1
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 9.5
-
pH 7.0 about 50% of maximal activity, pH 9.5 about 80% of maximal activity
7.5 - 10
-
pH 7.5 about 40% of maximal activity, pH 10 about 20% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10 - 50
-
about 40% of maximal activity at 10°C and about 10% of maximal activity at 50°C
24 - 37
-
about 50% of maximal activity at 25°C and about 30% of maximal activity at 37°C
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
