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expressed in Escherichia coli, strains BL 21 (DE3) and JM109, pCAMBIA 1300 vector used for construction of gene cassette for genetic transformation in Nicotiana tabacum, cyanamide hydratase (cah) used as a marker gene
the expression of the Cah enzyme in soybean leaves does not greatly affect the concentrations of any of the metabolites measured in unsprayed 1C and Jack plants grown under normal conditions or when placed in the dark for 24 h
transgenic soybean plants can be produced that express the fungal Cah gene and thus acquire resistance to toxic cyanamide. These plants are phenotypically normal and fertile indicating that expression of the Cah protein has no effect on soybean plant metabolism
wild-type cah and its mutants are cloned and overexpressed in pQE-60 Escherichia coli expression system. Western blot analysis confirms the production of 27700 Da cah enzyme by all the recombinants. Agrobacterium-mediated transformation is performed in Nicotiana tabaccum cv. Samsun plants by employing the leaf-disc method. The integration and expression of cah gene in transgenic plants are confirmed by polymerase chain reaction, Southern and Western blot analyses
due to its innate ability to convert cyanamide to urea and the broad-spectrum antimicrobial activity of cyanamide, the cah gene can be used to facilitate plant growth promotion and biocontrol of phytopathogens