We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. The enzyme uses fatty acyl thioesters of ACP in vivo. Different forms of the enzyme may have preferences for substrates with different chain length. For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyses EC 5.3.3.14, trans-2-decenoyl-[acyl-carrier protein] isomerase. Despite the differences both forms can catalyse all steps leading to the synthesis of palmitate (C16:0). FabZ, but not FabA, can also accept unsaturated substrates .
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
3-hydroxyacyl-ACP dehydratase,
AT5G60335 , beta-hydroxyacyl-[ACP] dehydratase, Beta-hydroxyoctanoyl-ACP-dehydrase, Beta-Hydroxyoctanoyl-acyl carrier protein dehydrase, D-3-Hydroxyoctanoyl-acyl carrier protein dehydratase, Dehydratase, beta-hydroxyoctanoyl thioester, Dehydratase, D-3-hydroxyoctanoyl-[acyl carrier protein], FabA, FabZ,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-hydroxyacyl-ACP dehydratase
beta-hydroxyacyl-[ACP] dehydratase
-
Beta-hydroxyoctanoyl-ACP-dehydrase
-
-
-
-
Beta-Hydroxyoctanoyl-acyl carrier protein dehydrase
-
-
-
-
D-3-Hydroxyoctanoyl-acyl carrier protein dehydratase
-
-
-
-
Dehydratase, beta-hydroxyoctanoyl thioester
-
-
-
-
Dehydratase, D-3-hydroxyoctanoyl-[acyl carrier protein]
-
-
-
-
3-hydroxyacyl-ACP dehydratase
component of the mitochondrial fatty acid synthase system
3-hydroxyacyl-ACP dehydratase
-
3-hydroxyacyl-ACP dehydratase
Q9L0M4; Q9L0M3
-
3-hydroxyacyl-ACP dehydratase
Q9L0M4; Q9L0M3
-
-
FabA
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
a (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MetaCyc
(5Z)-dodecenoate biosynthesis I , (5Z)-dodecenoate biosynthesis II , 8-amino-7-oxononanoate biosynthesis I , 8-amino-7-oxononanoate biosynthesis IV , anteiso-branched-chain fatty acid biosynthesis , cis-vaccenate biosynthesis , even iso-branched-chain fatty acid biosynthesis , fatty acid elongation -- saturated , gondoate biosynthesis (anaerobic) , mycolate biosynthesis , octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) , odd iso-branched-chain fatty acid biosynthesis , oleate biosynthesis IV (anaerobic) , palmitate biosynthesis II (type II fatty acid synthase) , palmitate biosynthesis III , palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) , stearate biosynthesis II (bacteria and plants) , stearate biosynthesis IV , streptorubin B biosynthesis , tetradecanoate biosynthesis (mitochondria)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3R)-3-hydroxyoctanoyl-[acyl-carrier protein] hydro-lyase (oct-2-enoyl-[acyl-carrier protein]-forming)
This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. The enzyme uses fatty acyl thioesters of ACP in vivo. Different forms of the enzyme may have preferences for substrates with different chain length. For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyses EC 5.3.3.14, trans-2-decenoyl-[acyl-carrier protein] isomerase. Despite the differences both forms can catalyse all steps leading to the synthesis of palmitate (C16:0). FabZ, but not FabA, can also accept unsaturated substrates [4].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3R)-3-hydroxybutyryl-[acyl-carrier protein]
2-butenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]
2-decenoyl-[acyl-carrier protein] + H2O
(3R)-3-hydroxydodecanoyl-[acyl-carrier protein]
2-dodecenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
(3R)-3-hydroxyheptanoyl-[acyl-carrier protein]
2-heptenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
(3R)-3-hydroxyhexanoyl-[acyl-carrier protein]
2-hexenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
(3R)-3-hydroxynonanoyl-[acyl-carrier protein]
2-nonenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
(3R)-3-hydroxyoctanoyl-[acyl-carrier protein]
2-octenoyl-[acyl-carrier protein] + H2O
-
beta-hydroxyoctanoyl-ACP
-
-
?
(3R)-3-hydroxyundecanoyl-[acyl-carrier protein]
2-undecenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
3-hydroxydecanoyl-N-acetylcysteamine
? + H2O
3-hydroxyhexanoyl-N-acetylcysteamine
? + H2O
3-hydroxyoctanoyl-N-acetylcysteamine
? + H2O
5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine
? + H2O
6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine
? + H2O
7-methyl-3-hydroxyoctanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
additional information
?
-
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]
2-decenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]
2-decenoyl-[acyl-carrier protein] + H2O
-
-
-
?
3-hydroxydecanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
3-hydroxydecanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
3-hydroxyhexanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
3-hydroxyhexanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
3-hydroxyoctanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
3-hydroxyoctanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine
? + H2O
Q9L0M4; Q9L0M3
the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins
-
-
?
additional information
?
-
-
no activity on C14- or C16-ACP derivatives
-
-
?
additional information
?
-
-
specific for 3-hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12, highest activity on C8 derivative
-
-
?
additional information
?
-
-
3-Hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]
2-decenoyl-[acyl-carrier protein] + H2O
-
-
-
?
(3R)-3-hydroxydodecanoyl-[acyl-carrier protein]
2-dodecenoyl-[acyl-carrier protein] + H2O
-
-
-
-
?
additional information
?
-
-
3-Hydroxyacyl-[acyl-carrier protein] derivatives, C6 to C12
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
p-chloromercuribenzoate
-
inhibition at 0.1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0685 - 0.09773
(3R)-3-hydroxybutyryl-[acyl-carrier protein]
0.0685
(3R)-3-hydroxybutyryl-[acyl-carrier protein]
pH 6.8, temperature not specified in the publication, HaHAD1 wild-type protein
0.0823
(3R)-3-hydroxybutyryl-[acyl-carrier protein]
pH 6.8, temperature not specified in the publication, HaHAD1 F166V mutant
0.09773
(3R)-3-hydroxybutyryl-[acyl-carrier protein]
pH 6.8, temperature not specified in the publication, HaHAD2 wild-type protein
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
226
3-hydroxydecanoyl-N-acetylcysteamine
Q9L0M4; Q9L0M3
pH 7.2, 25ưC
484
3-hydroxyhexanoyl-N-acetylcysteamine
Q9L0M4; Q9L0M3
pH 7.2, 25ưC
363
3-hydroxyoctanoyl-N-acetylcysteamine
Q9L0M4; Q9L0M3
pH 7.2, 25ưC
522
5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine
Q9L0M4; Q9L0M3
pH 7.2, 25ưC
527
6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine
Q9L0M4; Q9L0M3
pH 7.2, 25ưC
144
7-methyl-3-hydroxyoctanoyl-N-acetylcysteamine
Q9L0M4; Q9L0M3
pH 7.2, 25ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.2
Q9L0M4; Q9L0M3
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25
Q9L0M4; Q9L0M3
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.67
calculated from sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
-
UniProt
brenda
Q9L0M4: UPF0336 protein SCO4636, Q9L0M3: uncharacterized protein SCO4637
Q9L0M4; Q9L0M3
SwissProt
brenda
Q9L0M4: UPF0336 protein SCO4636, Q9L0M3: uncharacterized protein SCO4637
Q9L0M4; Q9L0M3
SwissProt
brenda
-
-
-
brenda
-
Swissprot
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
high activity
brenda
rosette
brenda
-
brenda
low activity
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
beta-hydroxyacyl-[acyl carrier protein (ACP)]-dehydratase is a component of the type II fatty acid synthase complex involved in de novo fatty acid biosynthesis in plants. Expression levels of HaHAD1 remains constant across most tissues suggest a housekeeping function
metabolism
beta-hydroxyacyl-[acyl carrier protein (ACP)]-dehydratase is a component of the type II fatty acid synthase complex involved in de novo fatty acid biosynthesis in plants. Higher levels of HaHAD2 in the initial stages of seed development inferr its key role in seed storage fatty acid synthesis
metabolism
important enzyme in controlling fatty acid chain length and saturation levels
metabolism
Q9L0M4; Q9L0M3
the enzyme also processes intermediates in both fatty acid and undecylprodiginine biosynthetic processes. It is capable of utilizing various chain lengths of straight- and branched-chain 3-hydroxyacyl-N-acetyl cysteamine substrates. FabA does not discriminate between acyl carrier proteins from primary and secondary metabolism. It processes intermediates for both biosynthetic pathways
metabolism
the enzyme catalyzes the third of the four iterative reactions that constitute the mitochondrial fatty acid synthase cycle
metabolism
-
the enzyme also processes intermediates in both fatty acid and undecylprodiginine biosynthetic processes. It is capable of utilizing various chain lengths of straight- and branched-chain 3-hydroxyacyl-N-acetyl cysteamine substrates. FabA does not discriminate between acyl carrier proteins from primary and secondary metabolism. It processes intermediates for both biosynthetic pathways
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
heterodimer
Q9L0M4; Q9L0M3
1 * 15000 + 1 * 20000, SDS-PAGE
heterodimer
-
1 * 15000 + 1 * 20000, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sitting drop vapor diffusion method, using 10 mM sodium phosphate (pH 8.0), 350 mM sodium acetate, 1 M LiCl and 35% (w/v) PEG3350
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
F165A
mutant enzyme shows decreased ability to discriminate between varying acyl chain lengths
F171A
mutant enzyme shows decreased ability to discriminate between varying acyl chain lengths
F21G
the mutant contains an expanded substrate pocket. This mutant is a possible means to produce both short and long chain fatty acids
F166V
the HaHAD1 F166V mutant protein has a Km for crotonyl-CoA of 0.823 mM and the Vmax 0.02161 mM/min*mg, indicatating that the mutant protein is more active with crotonyl-CoA than wild-type HaHAD1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60 - 65
-
20-30% loss of activity after 5 min
70
-
90% loss of activity after 5 min
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ni-affinity column chromatography
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli as soluble N-terminal His6-tag proteins
Q9L0M4; Q9L0M3
expression in yeast htd2 mutant
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
higher levels of HaHAD2 in the initial stages of seed development infer its key role in seed storage fatty acid synthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mizugaki, M.; Swindell, A.C.; Wakil, S.J.
Intermediate- and long-chain beta-hydroxyacyl-ACP dehydratases from E. coli fatty acid synthetase
Biochem. Biophys. Res. Commun.
33
520-527
1968
Escherichia coli
brenda
Nguyen, C.; Haushalter, R.; Lee, D.; Markwick, P.; Bruegger, J.; Caldara-Festin, G.; Finzel, K.; Jackson, D.; Ishikawa, F.; O'Dowd, B.; McCammon, J.; Opella, S.; Tsai, S.; Burkart, M.
Trapping the dynamic acyl carrier protein in fatty acid biosynthesis
Nature
505
427-431
2014
Escherichia coli (P0A6Q3), Escherichia coli
brenda
Finzel, K.; Nguyen, C.; Jackson, D.R.; Gupta, A.; Tsai, S.C.; Burkart, M.D.
Probing the substrate specificity and protein-protein interactions of the E. coli fatty acid dehydratase, FabA
Chem. Biol.
22
1453-1460
2015
Escherichia coli (P0A6Q3), Escherichia coli
brenda
Singh, R.; Reynolds, K.A.
Identification and characterization of FabA from the type II fatty acid synthase of Streptomyces coelicolor
J. Nat. Prod.
79
240-243
2016
Streptomyces coelicolor (Q9L0M4 AND Q9L0M3), Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471 (Q9L0M4 AND Q9L0M3)
brenda
Guan, X.; Okazaki, Y.; Lithio, A.; Li, L.; Zhao, X.; Jin, H.; Nettleton, D.; Saito, K.; Nikolau, B.J.
Discovery and characterization of the 3-hydroxyacyl-ACP dehydratase component of the plant mitochondrial fatty acid synthase system
Plant Physiol.
173
2010-2028
2017
Arabidopsis thaliana (Q9FJI2)
brenda
Gonzalez-Thuillier, I.; Venegas-Caleron, M.; Sanchez, R.; Garces, R.; von Wettstein-Knowles, P.; Martinez-Force, E.
Sunflower (Helianthus annuus) fatty acid synthase complex beta-hydroxyacyl-[acyl carrier protein] dehydratase genes
Planta
243
397-410
2016
Helianthus annuus (F2VYC9)
brenda
Select items on the left to see more content.
html completed