Information on EC 4.2.1.50 - Pyrazolylalanine synthase

for references in articles please use BRENDA:EC4.2.1.50
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
4.2.1.50
-
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RECOMMENDED NAME
GeneOntology No.
Pyrazolylalanine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-serine + pyrazole = 3-(pyrazol-1-yl)-L-alanine + H2O
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
L-serine hydro-lyase [adding pyrazole; 3-(pyrazol-1-yl)-L-alanine-forming]
A pyridoxal-phosphate protein.
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CAS REGISTRY NUMBER
COMMENTARY hide
37290-81-6
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
melon
-
-
Manually annotated by BRENDA team
cucumber
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-
Manually annotated by BRENDA team
pumpkin, marrow
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + H2O
show the reaction diagram
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-
-
-
L-Serine + pyrazole
?
show the reaction diagram
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-
-
-
-
O-Acetyl-L-serine + 3-amino-1,2,4-triazole
?
show the reaction diagram
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10.6% of activity with pyrazole
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-
-
O-Acetyl-L-serine + H2S
Cysteine + acetate
show the reaction diagram
-
-
-
-
O-Acetyl-L-serine + N-hydroxyurea
?
show the reaction diagram
-
8.7% of activity with pyrazole
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-
-
O-Acetyl-L-serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
O-Acetyl-L-serine + pyrazole
?
show the reaction diagram
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-
-
-
-
O-Succinyl-L-serine + pyrazole
?
show the reaction diagram
-
16.4% of activity with O-Acetyl-L-serine
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-
-
O-Sulfo-L-serine + pyrazole
?
show the reaction diagram
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2.5% of activity with O-Acetyl-L-serine
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-
-
additional information
?
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no activity with L-serine
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Serine + pyrazole
?
show the reaction diagram
-
-
-
-
-
O-Acetyl-L-serine + pyrazole
?
show the reaction diagram
-
-
-
-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
-
50 mM almost complete inhibition
potassium cyanide
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10 mM almost complete inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
O-acetyl-L-serine
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
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L-serine + pyrazole
7.3 - 7.4
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O-acetyl-L-serine + pyrazole
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1 - 7.7
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38% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.1 and 56% of maximal activity at pH 7.7
6.5 - 8.5
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50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
2 * 32000, SDS-PAGE
58000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 32000, SDS-PAGE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to freezing even in 20% glycerol
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
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production of beta-(pyrazol-1-yl)-L-alanine for pharmacological use by enzyme overexpressed in E. coli
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LINKS TO OTHER DATABASES (specific for EC-Number 4.2.1.50)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)