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Information on EC 4.2.1.50 - pyrazolylalanine synthase for references in articles please use BRENDA:EC4.2.1.50
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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
beta-(1-Pyrazol-1-yl)-L-alanine synthase, beta-(1-Pyrazolyl)alanine synthase, beta-(Pyrazol-1-yl)-L-alanine /L-cysteine synthase, beta-Ppyrazol-1-ylalanine synthetase, beta-Pyrazolylalaninase, Cystein synthase, Pyrazolealanine synthase,
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beta-(1-Pyrazol-1-yl)-L-alanine synthase
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beta-(1-Pyrazolyl)alanine synthase
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beta-(Pyrazol-1-yl)-L-alanine /L-cysteine synthase
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beta-Ppyrazol-1-ylalanine synthetase
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beta-Pyrazolylalaninase
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Pyrazolealanine synthase
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L-serine + pyrazole = 3-(pyrazol-1-yl)-L-alanine + H2O
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L-serine hydro-lyase [adding pyrazole; 3-(pyrazol-1-yl)-L-alanine-forming]
A pyridoxal-phosphate protein.
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L-Serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + H2O
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L-Serine + pyrazole
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O-Acetyl-L-serine + 3-amino-1,2,4-triazole
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10.6% of activity with pyrazole
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O-Acetyl-L-serine + H2S
Cysteine + acetate
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O-Acetyl-L-serine + N-hydroxyurea
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8.7% of activity with pyrazole
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O-Acetyl-L-serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + acetate
O-Acetyl-L-serine + pyrazole
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O-Succinyl-L-serine + pyrazole
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16.4% of activity with O-Acetyl-L-serine
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O-Sulfo-L-serine + pyrazole
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2.5% of activity with O-Acetyl-L-serine
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additional information
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no activity with L-serine
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O-Acetyl-L-serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + acetate
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O-Acetyl-L-serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + acetate
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L-Serine + pyrazole
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O-Acetyl-L-serine + pyrazole
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pyridoxal 5'-phosphate
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1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
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hydroxylamine
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50 mM almost complete inhibition
potassium cyanide
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10 mM almost complete inhibition
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2.5
O-acetyl-L-serine
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6.7
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L-serine + pyrazole
7.3 - 7.4
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O-acetyl-L-serine + pyrazole
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6.1 - 7.7
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38% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.1 and 56% of maximal activity at pH 7.7
6.5 - 8.5
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50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
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water melon
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brenda
melon
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brenda
cucumber
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brenda
pumpkin, marrow
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brenda
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brenda
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brenda
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brenda
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CYSK_CITLA
325
0
34343
Swiss-Prot
A0A1S7SGV8_9RHIZ
318
0
34722
TrEMBL
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32000
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2 * 32000, SDS-PAGE
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dimer
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2 * 32000, SDS-PAGE
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sensitive to freezing even in 20% glycerol
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expression in Escherichia coli
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pharmacology
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production of beta-(pyrazol-1-yl)-L-alanine for pharmacological use by enzyme overexpressed in E. coli
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Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.
Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris
Phytochemistry
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973-977
1984
Citrullus lanatus subsp. vulgaris
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brenda
Dunnill, P.M.; Fowden, L.
The biosynthesis of bety-pyrazol-1-ylalanine
J. Exp. Bot.
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237-248
1963
Cucumis melo, Cucumis sativus, Cucurbita pepo
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brenda
Noji, M.; Murakoshi, I.; Saito, K.
Evidence for identity of beta-pyrazolealanine synthase with cysteine synthase in watermelon: Formation of beta-pyrazolealanine by cloned cysteine synthase in vitro and in vivo
Biochem. Biophys. Res. Commun.
197
1111-1117
1993
Citrullus lanatus subsp. vulgaris
brenda
Saito, K.; Kimura, N.; Ikegami, F.; Noji, M.
Production of plant non-protein amino acids by recombinant enzymes of sequential biosynthetic reactions in bacteria
Biol. Pharm. Bull.
20
47-53
1997
Citrullus lanatus subsp. vulgaris
brenda
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