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Information on EC 4.2.1.47 - GDP-mannose 4,6-dehydratase and Organism(s) Arabidopsis thaliana and UniProt Accession P93031

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.47 GDP-mannose 4,6-dehydratase
IUBMB Comments
The bacterial enzyme requires bound NAD+. This enzyme forms the first step in the biosynthesis of GDP-alpha-D-rhamnose and GDP-beta-L-fucose. In Aneurinibacillus thermoaerophilus L420-91T, this enzyme acts as a bifunctional enzyme, catalysing the above reaction as well as the reaction catalysed by EC 1.1.1.281, GDP-4-dehydro-6-deoxy-D-mannose reductase . Belongs to the short-chain dehydrogenase/reductase enzyme family, having homologous structures and a conserved catalytic triad of Lys, Tyr and Ser/Thr residues .
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Arabidopsis thaliana
UNIPROT: P93031
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
gdp-mannose dehydrogenase, gdp-mannose 4,6-dehydratase, gdp-d-mannose 4,6-dehydratase, gdp-d-mannose-4,6-dehydratase, gdp-mannose-4,6-dehydratase, gdp-mannose dehydratase, gdp-mannose 4,6 dehydratase, gdp-d-mannose dehydratase, pbcv-1 gmd, m-gmd, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GDP-mannose dehydrogenase
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Dm-gmd
-
-
-
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GDP-D-mannose 4,6-dehydratase
-
-
-
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GDP-D-mannose 4-oxido-6-reductase
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-
-
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GDP-D-mannose dehydratase
-
-
-
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GDP-mannose 4,6-dehydratase
-
-
-
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GDP-mannose dehydratase
-
-
-
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GDP-mannose dehydrogenase
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GMD
-
-
-
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Guanosine 5'-diphosphate-D-mannose oxidoreductase
-
-
-
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Guanosine diphosphomannose 4,6-dehydratase
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-
-
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Guanosine diphosphomannose oxidoreductase
-
-
-
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ORF13.7
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose 4,6-hydro-lyase (GDP-4-dehydro-6-deoxy-D-mannose-forming)
The bacterial enzyme requires bound NAD+. This enzyme forms the first step in the biosynthesis of GDP-alpha-D-rhamnose and GDP-beta-L-fucose. In Aneurinibacillus thermoaerophilus L420-91T, this enzyme acts as a bifunctional enzyme, catalysing the above reaction as well as the reaction catalysed by EC 1.1.1.281, GDP-4-dehydro-6-deoxy-D-mannose reductase [5]. Belongs to the short-chain dehydrogenase/reductase enzyme family, having homologous structures and a conserved catalytic triad of Lys, Tyr and Ser/Thr residues [6].
CAS REGISTRY NUMBER
COMMENTARY hide
37211-59-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-mannose
GDP-4-dehydro-6-deoxy-D-mannose + H2O
show the reaction diagram
-
-
?
GDPmannose
?
show the reaction diagram
-
-
-
?
GDPmannose
GDP-4-dehydro-6-deoxy-D-mannose + H2O
show the reaction diagram
-
-
?
GDP-mannose
GDP-4-dehydro-6-deoxy-D-mannose + H2O
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDPmannose
?
show the reaction diagram
-
-
-
?
additional information
?
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GMD2_ARATH
373
0
41961
Swiss-Prot
other Location (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
gel filtration, crystallization experiments
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, complexed with NADPH, GDP and GDP-D-rhamnose
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Agrobacterium tumefaciens
expression in Escherichia coli
coexpression with GDP-4-keto-6-deioxymannose-3,5-epimerase-4-reductase, which is required for enzymatic activity after expresseion in Saccharomyces cerevisiae
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expression in Agrobacterium tumefaciens
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bonin, C.P.; Potter, I.; Vanzin, G.F.; Reiter, W.D.
The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose-4,6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose
Proc. Natl. Acad. Sci. USA
94
2085-2090
1997
Arabidopsis thaliana (P93031), Arabidopsis thaliana
Manually annotated by BRENDA team
Mulichak, A.M.; Bonin, C.P.; Reiter, W.D.; Garavito, R.M.
Structure of the MUR1 GDP-mannose 4,6-dehydratase from Arabidopsis thaliana: implications for ligand binding and specificity
Biochemistry
41
15578-15589
2002
Arabidopsis thaliana (P93031), Arabidopsis thaliana
Manually annotated by BRENDA team
Nakayama, K.; Maeda, Y.; Jigami, Y.
Interaction of GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase with GDP-mannose-4,6-dehydratase stabilizes the enzyme activity for formation of GDP-fucose from GDP-mannose
Glycobiology
13
673R-680R
2003
Arabidopsis thaliana
-
Manually annotated by BRENDA team
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (P93031), Arabidopsis thaliana (Q9SNY3)
Manually annotated by BRENDA team