Information on EC 4.2.1.40 - glucarate dehydratase

for references in articles please use BRENDA:EC4.2.1.40
Word Map on EC 4.2.1.40
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.40
-
RECOMMENDED NAME
GeneOntology No.
glucarate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-glucarate degradation I
-
-
D-glucarate degradation II
-
-
degradation of sugar acids
-
-
Ascorbate and aldarate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glucarate hydro-lyase (5-dehydro-4-deoxy-D-glucarate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9059-02-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Paracolobactrum sp.
-
-
-
Manually annotated by BRENDA team
A
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-deoxy-4-fluoro-D-glucarate
?
show the reaction diagram
D-glucarate
3-deoxy-L-threo-2-hexulosarate
show the reaction diagram
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
show the reaction diagram
D-Glucarate
?
show the reaction diagram
D-glucarate
D-idarate
show the reaction diagram
D-idarate
D-glucarate
show the reaction diagram
D-idarate
L-glucarate
show the reaction diagram
-
-
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
show the reaction diagram
D-Glucarate
?
show the reaction diagram
D-idarate
L-glucarate
show the reaction diagram
-
-
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
less than one-third as effective as Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-tartrate
-
2.5 mM, 58% inhibition
(-)-tartrate
-
2.5 mM, 5% inhibition
4-deoxy-D-glucarate
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5-Amino-1H-tetrazole
-
-
Co2+
-
high concentrations
D-idaric acid
-
-
galactaric acid
-
-
meso-tartrate
-
2.5 mM, 31% inhibition
Mn2+
-
high concentrations
p-chloromercuribenzene sulfonate
xylarohydroxamate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 1.42
D-glucarate
0.016
L-idarate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28 - 44
4-deoxy-4-fluoro-D-glucarate
0.00033 - 35
D-glucarate
0.00025 - 35
L-idarate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay method
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
-
Tris-maleate buffer
7.5
-
barbital buffer
7.5 - 8.5
-
Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
activity assay
PDB
SCOP
CATH
UNIPROT
ORGANISM
A6VQF1
Actinobacillus succinogenes (strain ATCC 55618 / 130Z);
Q39KL8
Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383);
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768);
Escherichia coli (strain K12);
A4XRL3
Pseudomonas mendocina (strain ymp);
P42206
Pseudomonas putida;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34960
calculated
38000
determined by SDS-PAGE
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method
crystal structure at 2.3 A resolution
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6
-
moderately stable
33840
7.5
-
most stable near
33840
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D366A
-
reduced kcat for D-glucarate and L-idarate
D366N
-
reduced kcat for D-glucarate and 3fold reduced kcat for L-idarate
H339A
-
reduced kcat for D-glucarate and L-idarate
H339N
-
reduced kcat for D-glucarate and L-idarate
H339Q
-
reduced kcat for D-glucarate and L-idarate
K207Q
-
reduced kcat for D-glucarate and L-idarate
K207R
-
reduced kcat for D-glucarate and L-idarate
N341D
-
do not catalyze the dehydrofluorination of 4-deoxy-4-fluoro-D-glucarate
N341L
-
catalyzes the dehydrofluorination of 4-deoxy-4-fluoro-D-glucarate
Y150F
-
significant reduction of kcat for D-glucarate and L-idarate
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
Show AA Sequence (1488 entries)
Please use the Sequence Search for a specific query.