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Information on EC 4.2.1.3 - aconitate hydratase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SIB9

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.3 aconitate hydratase
IUBMB Comments
Besides interconverting citrate and cis-aconitate, it also interconverts cis-aconitate with isocitrate and, hence, interconverts citrate and isocitrate. The equilibrium mixture is 91% citrate, 6% isocitrate and 3% aconitate. cis-Aconitate is used to designate the isomer (Z)-prop-1-ene-1,2,3-tricarboxylate. An iron-sulfur protein, containing a [4Fe-4S] cluster to which the substrate binds.
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Arabidopsis thaliana
UNIPROT: Q9SIB9
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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citrate
=
cis-aconitate
+
H2O
=
+
Synonyms
aconitase, iron regulatory protein, irp-1, ire-bp, macon, iron regulatory protein 1, aconitate hydratase, cytoplasmic aconitase, aconitase a, c-aconitase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aconitase
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-
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Aconitate hydratase
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-
-
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cis-aconitase
-
-
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citrate hydro-lyase
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-
-
-
Ferritin repressor protein
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-
-
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hydratase, aconitate
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-
-
-
IP210
-
-
-
-
IRE-BP
-
-
-
-
Iron regulatory protein
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-
-
-
iron-responsive element binding protein
-
-
-
-
IRP
-
-
-
-
IRP1
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-
-
-
Major iron-containing protein
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-
-
-
MICP
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
citrate(isocitrate) hydro-lyase (cis-aconitate-forming)
Besides interconverting citrate and cis-aconitate, it also interconverts cis-aconitate with isocitrate and, hence, interconverts citrate and isocitrate. The equilibrium mixture is 91% citrate, 6% isocitrate and 3% aconitate. cis-Aconitate is used to designate the isomer (Z)-prop-1-ene-1,2,3-tricarboxylate. An iron-sulfur protein, containing a [4Fe-4S] cluster to which the substrate binds.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-25-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
additionally to catalytic activity, enzyme is able to bind specifically the 5’ UTP of the Arabidopsis chloroplastic CuZn superoxide dismutase 2 mRNA. Enzyme does not bind an iron responsive element of the human ferritin gene
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression is transiently induced during germination
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
more than 90% of ACO3 activity is cytosolic. An iron-sulfur centre assembly mutant atm3-1 shows reduced cytosolic ACO activity
Manually annotated by BRENDA team
-
isoforms Aco1, Aco2, Aco3. Cytosolic aconitase is not converted into an iron-responsive element and does not regulate iron homeostasis
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the Aco3 mutant shows delayed early seedling growth, altered assimilation of [14C]acetate feeding and elevated citrate levels, which are nearly 4fold greater than in wild-type, or isoforms Aco1 or Aco2 mutants
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ACO3M_ARATH
990
0
108201
Swiss-Prot
Chloroplast (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Arnaud, N.; Ravet, K.; Borlotti, A.; Touraine, B.; Boucherez, J.; Fizames, C.; Briat, J.F.; Cellier, F.; Gaymard, F.
The iron-responsive element (IRE)/iron-regulatory protein 1 (IRP1)-cytosolic aconitase iron-regulatory switch does not operate in plants
Biochem. J.
405
523-531
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Moeder, W.; Del Pozo, O.; Navarre, D.A.; Martin, G.B.; Klessig, D.F.
Aconitase plays a role in regulating resistance to oxidative stress and cell death in Arabidopsis and Nicotiana benthamiana
Plant Mol. Biol.
63
273-287
2007
Arabidopsis thaliana, Nicotiana benthamiana
Manually annotated by BRENDA team
Hooks, M.A.; Allwood, J.W.; Harrison, J.K.; Kopka, J.; Erban, A.; Goodacre, R.; Balk, J.
Selective induction and subcellular distribution of ACONITASE 3 reveal the importance of cytosolic citrate metabolism during lipid mobilization in Arabidopsis
Biochem. J.
463
309-317
2014
Arabidopsis thaliana (Q9SIB9), Arabidopsis thaliana
Manually annotated by BRENDA team