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IUBMB Comments The enzyme, purified from the bacterium Thauera aromatica , is involved in an anaerobic toluene degradation pathway in which it catalyses the hydration of (E )-benzylidenesuccinyl-CoA.
Synonyms bbsH , enoyl-CoA hydratase, phenylitaconyl-CoA hydratase,
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phenylitaconyl-CoA hydratase
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bbsH
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(R,S)-2-(alpha-hydroxybenzyl)succinyl-CoA = (E)-benzylidenesuccinyl-CoA + H2O
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MetaCyc
toluene degradation to benzoyl-CoA (anaerobic)
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(R,S)-2-(alpha-hydroxybenzyl)succinyl-CoA hydro-lyase
The enzyme, purified from the bacterium Thauera aromatica, is involved in an anaerobic toluene degradation pathway in which it catalyses the hydration of (E)-benzylidenesuccinyl-CoA.
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(E)-2-benzylidenesuccinyl-NAC + H2O
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Substrates: -
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(E)-benzylidenesuccinyl-CoA + H2O
(R,S)-2-(alpha-hydroxybenzyl)succinyl-CoA
Substrates: -
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phenylitaconyl-CoA + H2O
2-carboxymethyl-3-hydroxy-3-phenylpropionyl-CoA + H2O
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Substrates: -
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phenylitaconyl-CoA + H2O
2-carboxymethyl-3-hydroxy-3-phenylpropionyl-CoA + H2O
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Substrates: -
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(E)-2-benzylidenesuccinyl-NAC
substrate inhibition
(E)-benzylidenesuccinyl-CoA
substrate inhibition
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0.226
(E)-2-benzylidenesuccinyl-NAC
at pH 6.2 and 30°C
0.23
(E)-benzylidenesuccinyl-CoA
at pH 6.2 and 30°C
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1.4
cell extract, at pH 6.2 and 30°C
27
after 19fold enrichment, at pH 6.2 and 30°C
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6.2
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isoelectric focusing
6.2
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calculated from amino acid sequence
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brenda
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UniProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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BBSH_THAAR
256
0
27419
Swiss-Prot
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homotetramer
4 * 28 000, SDS-PAGE
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x * 27300, calculated from amino acid sequence
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DEAE-Sepharose column chromatography and hydroxyapatite column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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Leuthner, B.; Heider, J.
Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of b oxidation of the intermediate benzylsuccinate
J. Bacteriol.
182
272-277
2000
Thauera aromatica
brenda
von Horsten, S.; Lippert, M.L.; Geisselbrecht, Y.; Schuehle, K.; Schall, I.; Essen, L.O.; Heider, J.
Inactive pseudoenzyme subunits in heterotetrameric BbsCD, a novel short-chain alcohol dehydrogenase involved in anaerobic toluene degradation
FEBS J.
289
1023-1042
2022
Thauera aromatica (Q9KJE7)
brenda
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