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Information on EC 4.2.1.179 - difructose-anhydride-I synthase
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EC Tree
4.2.1.179 difructose-anhydride-I synthaseIUBMB Comments
The enzyme, studied in the fungus Aspergillus fumigatus, may participate in an inulin degradation pathway in which it converts the product of EC 4.2.2.17, inulin fructotransferase (DFA-I-forming), to inulobiose, though in vitro activity was higher in the direction of DFA-I formation.
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
alphaFFase1, BBDE_2040, bis-D-fructose 2',1:2,1'-dianhydride fructohydrolase, DFA I synthase/hydrolase, DFAIase, difructose anhydride I hydrolase, difructose dianhydride I synthase/hydrolase, EC 3.2.1.134, inulobiose hydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alphaFFase1
BBDE_2040
bis-D-fructose 2',1:2,1'-dianhydride fructohydrolase
-
-
-
-
DFA I synthase/hydrolase
DFAIase
-
-
-
-
difructose dianhydride I synthase/hydrolase
EC 3.2.1.134
-
-
formerly
-
inulobiose hydrolase
-
-
-
-
alphaFFase1
-
bifunctional enzyme with alpha-D-fructofuranosidase and difructose dianhydride I synthase/hydrolase activities
alphaFFase1
-
bifunctional enzyme with alpha-D-fructofuranosidase and difructose dianhydride I synthase/hydrolase activities
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
inulobiose = bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
inulobiose hydro-lyase (alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,1'-dianhydride-forming)
The enzyme, studied in the fungus Aspergillus fumigatus, may participate in an inulin degradation pathway in which it converts the product of EC 4.2.2.17, inulin fructotransferase (DFA-I-forming), to inulobiose, though in vitro activity was higher in the direction of DFA-I formation.
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CAS REGISTRY NUMBER
COMMENTARY
121479-55-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
inulobiose
difructose dianhydride I + H2O
-
i.e. beta-D-Fruf-(2->1)-alpha-D-Fruf
-
-
r
1-methyl alpha-D-arabinofuranoside + H2O
alpha-D-arabinose + methanol
-
-
-
-
?
1-methyl alpha-D-arabinofuranoside + H2O
alpha-D-arabinose + methanol
-
-
-
-
?
1-methyl alpha-D-fructofuranoside + H2O
alpha-D-fructose + methanol
-
-
-
-
?
1-methyl alpha-D-fructofuranoside + H2O
alpha-D-fructose + methanol
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-
-
-
?
4-nitrophenyl alpha-D-arabinofuranoside + H2O
4-nitrophenol + alpha-D-arabinofuranose
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-
-
-
?
4-nitrophenyl alpha-D-arabinofuranoside + H2O
4-nitrophenol + alpha-D-arabinofuranose
-
-
-
-
?
beta-D-Frup-(2->1)-alpha-D-Fruf
alpha-D-Fruf-1,2':2,1'-beta-D-Frup + H2O
-
-
-
-
?
beta-D-Frup-(2->1)-alpha-D-Fruf
alpha-D-Fruf-1,2':2,1'-beta-D-Frup + H2O
-
-
-
-
?
bis-D-fructose 2',1:2,1'-dianhydride + H2O
inulobiose
-
i.e. difructose anhydride I
-
-
r
bis-D-fructose 2',1:2,1'-dianhydride + H2O
inulobiose
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the equilibrium of the enzymic reaction is at about 85% in the cyclization and about 15% in the hydrolysis
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-
r
inulobiose
bis-D-fructose 2',1:2,1' dianhydride + H2O
-
might act as a regulator for a piecemeal use of inulin as a carbon source
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-
r
inulobiose
bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
the equilibrium of the enzymic reaction is at about 85% in the cyclization and about 15% in the hydrolysis
-
-
r
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
bis-D-fructose 2',1:2,1'-dianhydride + H2O
inulobiose
-
i.e. difructose anhydride I
-
-
r
inulobiose
bis-D-fructose 2',1:2,1' dianhydride + H2O
-
might act as a regulator for a piecemeal use of inulin as a carbon source
-
-
r
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
Ca2+, Mg2+, and Mn2+ have no effect on the enzyme activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
EDTA does not inhibit the activity of the enzyme
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexamer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M disodium tartrate
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D292A
-
the mutant shows 1.3% activity with inulobiose compared to the wild type enzyme
D292N
-
the mutant shows 0.1% activity with inulobiose compared to the wild type enzyme
E270A
-
the mutant shows 0.1% activity with inulobiose compared to the wild type enzyme
E291Q
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the mutant shows 2.2% activity with inulobiose compared to the wild type enzyme
E85A
-
the mutant shows 0.5% activity with inulobiose compared to the wild type enzyme
E85Q
-
the mutant shows 3% activity with inulobiose compared to the wild type enzyme
K147A
-
the mutant shows 4.8% activity with inulobiose compared to the wild type enzyme
W298A
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the mutant shows 4.1% activity with inulobiose compared to the wild type enzyme
Y187A
-
the mutant shows 3.2% activity with inulobiose compared to the wild type enzyme
Y187F
-
the mutant shows 30.7% activity with inulobiose compared to the wild type enzyme
D292A
-
the mutant shows 1.3% activity with inulobiose compared to the wild type enzyme
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D292N
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the mutant shows 0.1% activity with inulobiose compared to the wild type enzyme
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E85A
-
the mutant shows 0.5% activity with inulobiose compared to the wild type enzyme
-
E85Q
-
the mutant shows 3% activity with inulobiose compared to the wild type enzyme
-
K147A
-
the mutant shows 4.8% activity with inulobiose compared to the wild type enzyme
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
50
-
the enzyme is stable up to 50°C at pH 6.0, with Tris-malate buffer
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
under toluene in the refrigerator at pH 6.0, stable for several months
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complete His-Tag purification resin column chromatography and Superdex 200 gel filtration
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DEAE-cellulose column chromatography and Sephadex G-200 gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matsuyama, T.; Tanaka, K.
On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose
Agric. Biol. Chem.
53
831-832
1989
Aspergillus fumigatus
-
brenda
Matsuyama, T.; Tanaka, K.; Mashiko, M.; Kanamoto, M.
Enzymic formation of di-D-fructose 1,2; 2,1 dianhydride from inulobiose by Aspergillus fumigatus
J. Biochem.
92
1325-1328
1982
Aspergillus fumigatus
brenda
Matsuyama, T.; Tanaka, K.
On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose
Agric. Biol. Chem.
53
831-832
1989
Aspergillus fumigatus
-
brenda
Matsuyama, T.; Tanaka, K.; Mashiko, M.; Kanamoto, M.
Enzymic formation of di-D-fructose 1,2 2,1 dianhydride from inulobiose by Aspergillus fumigatus
J. Biochem.
92
1325-1328
1982
Aspergillus fumigatus
brenda
Kashima, T.; Okumura, K.; Ishiwata, A.; Kaieda, M.; Terada, T.; Arakawa, T.; Yamada, C.; Shimizu, K.; Tanaka, K.; Kitaoka, M.; Ito, Y.; Fujita, K.; Fushinobu, S.
Identification of difructose dianhydride I synthase/hydrolase from an oral bacterium establishes a novel glycoside hydrolase family
J. Biol. Chem.
297
101324
2021
Bifidobacterium dentium, Bifidobacterium dentium JCM 1195
brenda
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Release 2023.1 (January 2023)
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