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IUBMB CommentsThe enzyme from the bacterium Chloroflexus aurantiacus is part of the 3-hydroxypropanoate cycle for carbon assimilation.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
mEH, mesaconyl-C4-CoA hydratase, mesaconyl-CoA hydratase, mesaconyl-coenzyme A hydratase,
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mesaconyl-C4-CoA hydratase
mesaconyl-coenzyme A hydratase
mEH
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mesaconyl-C4-CoA hydratase
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mesaconyl-C4-CoA hydratase
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mesaconyl-CoA hydratase
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mesaconyl-CoA hydratase
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mesaconyl-coenzyme A hydratase
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ambiguous
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mesaconyl-coenzyme A hydratase
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mesaconyl-coenzyme A hydratase
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(S)-citramalyl-CoA = 3-methylfumaryl-CoA + H2O
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(S)-citramalyl-CoA hydro-lyase (3-methylfumaryl-CoA-forming)
The enzyme from the bacterium Chloroflexus aurantiacus is part of the 3-hydroxypropanoate cycle for carbon assimilation.
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beta-methylmalyl-CoA
mesaconyl-CoA + H2O
mesaconyl-C4-CoA + H2O
(S)-citramalyl-CoA
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beta-methylmalyl-CoA
mesaconyl-CoA + H2O
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beta-methylmalyl-CoA
mesaconyl-CoA + H2O
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beta-methylmalyl-CoA
mesaconyl-CoA + H2O
mesaconyl-C4-CoA + H2O
(S)-citramalyl-CoA
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beta-methylmalyl-CoA
mesaconyl-CoA + H2O
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beta-methylmalyl-CoA
mesaconyl-CoA + H2O
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brenda
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brenda
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UniProt
brenda
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30000
x * 30000, SDS-PAGE
37000
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x * 37000, SDS-PAGE
38000
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x * 38000, SDS-PAGE
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x * 37000, SDS-PAGE
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Ni-Sepharose column chromatography
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Ni-Sepharose column chromatography and Superdex 200 gel filtration
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expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli BL21 cells
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Zarzycki, J.; Schlichting, A.; Strychalsky, N.; Muller, M.; Alber, B.E.; Fuchs, G.
Mesaconyl-coenzyme A hydratase, a new enzyme of two central carbon metabolic pathways in bacteria
J. Bacteriol.
190
1366-1374
2008
Chloroflexus aurantiacus, Cereibacter sphaeroides
brenda
Zarzycki, J.; Brecht, V.; Muller, M.; Fuchs, G.
Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus
Proc. Natl. Acad. Sci. USA
106
21317-21322
2009
Chloroflexus aurantiacus (A9WC41)
brenda
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4.2.1.153 3-methylfumaryl-CoA hydratase
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