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EC Tree
IUBMB Comments Found in methylotrophic bacteria and methanogenic archaea.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
FAE, formaldehyde-activating enzyme,
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formaldehyde-activating enzyme
FAE
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formaldehyde-activating enzyme
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formaldehyde-activating enzyme
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5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O
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5,6,7,8-tetrahydromethanopterin hydro-lyase (formaldehyde-adding, tetrahydromethanopterin-forming)
Found in methylotrophic bacteria and methanogenic archaea.
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
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?
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tetrahydromethanopterin
dependent on
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Mg2+
stimulates activity, Ka: 4 mM
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1
5,6,7,8-tetrahydromethanopterin
30°C, pH 8.0
0.2
formaldehyde
30°C, pH 8.0
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SwissProt
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SwissProt
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one of the major proteins in the cytoplasm
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one of the major proteins in the cytoplasm
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malfunction
mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde
malfunction
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mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde
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physiological function
the enzyme is required for growth on methanol
physiological function
formaldehyde-activating enzyme is essential for energy metabolism and formaldehyde detoxification
physiological function
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the enzyme is required for growth on methanol
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physiological function
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formaldehyde-activating enzyme is essential for energy metabolism and formaldehyde detoxification
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18000
3 * 18000, SDS-PAGE
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homopentamer
x-ray crystallography
homopentamer
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x-ray crystallography
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homotrimer
3 * 18000, SDS-PAGE
homotrimer
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3 * 18000, SDS-PAGE
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non-labeled and selenomethionine-labeled enzyme, hanging drop vapor diffusion method, using 0.2 M calcium chloride hydrate, 0.1 M sodium acetate hydrate, pH 4.6, and 10-20% (v/v) isopropanol
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expressed in Escherichia coli BL21(DE3) pLysS cells
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Vorholt, J.A.; Marx, C.J.; Lidstrom, M.E.; Thauer, R.K.
Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol
J. Bacteriol.
182
6645-6650
2000
Methylorubrum extorquens (Q9FA38), Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (Q9FA38)
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Acharya, P.; Goenrich, M.; Hagemeier, C.H.; Demmer, U.; Vorholt, J.A.; Thauer, R.K.; Ermler, U.
How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1
J. Biol. Chem.
280
13712-13719
2005
Methylorubrum extorquens (Q9FA38), Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (Q9FA38)
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4.2.1.147 5,6,7,8-tetrahydromethanopterin hydro-lyase
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