We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 4.2.1.147 - 5,6,7,8-tetrahydromethanopterin hydro-lyase for references in articles please use BRENDA:EC4.2.1.147
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Found in methylotrophic bacteria and methanogenic archaea.
The enzyme appears in viruses and cellular organisms
Synonyms
FAE, formaldehyde-activating enzyme,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
formaldehyde-activating enzyme
FAE
-
formaldehyde-activating enzyme
-
formaldehyde-activating enzyme
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,6,7,8-tetrahydromethanopterin hydro-lyase (formaldehyde-adding, tetrahydromethanopterin-forming)
Found in methylotrophic bacteria and methanogenic archaea.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
?
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
tetrahydromethanopterin
-
dependent on
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
-
stimulates activity, Ka: 4 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1
5,6,7,8-tetrahydromethanopterin
-
30°C, pH 8.0
0.2
formaldehyde
-
30°C, pH 8.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
one of the major proteins in the cytoplasm
brenda
-
one of the major proteins in the cytoplasm
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
-
mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde
malfunction
-
mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde
-
physiological function
-
the enzyme is required for growth on methanol
physiological function
-
formaldehyde-activating enzyme is essential for energy metabolism and formaldehyde detoxification
physiological function
-
formaldehyde-activating enzyme is essential for energy metabolism and formaldehyde detoxification; the enzyme is required for growth on methanol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
18000
-
3 * 18000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
homopentamer
-
x-ray crystallography
homopentamer
-
x-ray crystallography
-
homotrimer
-
3 * 18000, SDS-PAGE
homotrimer
-
3 * 18000, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
non-labeled and selenomethionine-labeled enzyme, hanging drop vapor diffusion method, using 0.2 M calcium chloride hydrate, 0.1 M sodium acetate hydrate, pH 4.6, and 10-20% (v/v) isopropanol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) pLysS cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Vorholt, J.A.; Marx, C.J.; Lidstrom, M.E.; Thauer, R.K.
Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol
J. Bacteriol.
182
6645-6650
2000
Methylorubrum extorquens (Q9FA38), Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (Q9FA38)
brenda
Acharya, P.; Goenrich, M.; Hagemeier, C.H.; Demmer, U.; Vorholt, J.A.; Thauer, R.K.; Ermler, U.
How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1
J. Biol. Chem.
280
13712-13719
2005
Methylorubrum extorquens, Methylorubrum extorquens (Q9FA38), Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (Q9FA38)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
4.2.1.147 5,6,7,8-tetrahydromethanopterin hydro-lyase
more
top
Information
