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Information on EC 4.2.1.133 - copal-8-ol diphosphate hydratase and Organism(s) Cistus creticus and UniProt Accession E2IHE0

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.133 copal-8-ol diphosphate hydratase
IUBMB Comments
Requires Mg2+. The enzyme was characterized from the plant Cistus creticus subsp. creticus.
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This record set is specific for:
Cistus creticus
UNIPROT: E2IHE0
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Word Map
The taxonomic range for the selected organisms is: Cistus creticus
The enzyme appears in selected viruses and cellular organisms
Synonyms
cftps2, cccls, twtps21, copal-8-ol diphosphate synthase, nt-cps2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copal-8-ol diphosphate synthase
-
CcCLS
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl-diphosphate hydro-lyase [(13E)-8alpha-hydroxylabda-13-en-15-yl diphosphate forming]
Requires Mg2+. The enzyme was characterized from the plant Cistus creticus subsp. creticus.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subsp. creticus, gene CcCLS
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
of young leaves
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
protein-based phylogenetic analysis of plant diterpene synthases, overview
physiological function
the enzyme is responsible for synthesis of copal-8-ol diphosphate, which is likely to be an intermediate in the biosynthesis of the oxygen-containing labdane-type diterpenes that are abundant in the resin of this plant. CLS is a putative key enzyme in the biosynthesis of oxygen-containing labdane-type diterpenes
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant CcCLS from Escherichia coli by affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene CcCLS, DNA and amino acid sequence determination, analysis, and comparisons, cloning from cDNA library, expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is increased by wounding of leaves
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Falara, V.; Pichersky, E.; Kanellis, A.K.
A copal-8-ol diphosphate synthase from the angiosperm Cistus creticus subsp. creticus is a putative key enzyme for the formation of pharmacologically active, oxygen-containing labdane-type diterpenes
Plant Physiol.
154
301-310
2010
Cistus creticus (E2IHE0), Cistus creticus
Manually annotated by BRENDA team