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Information on EC 4.2.1.130 - D-lactate dehydratase

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.130 D-lactate dehydratase
IUBMB Comments
The enzyme, described from the fungi Candida albicans and Schizosaccharomyces pombe, converts 2-oxopropanal to (R)-lactate in a single glutathione (GSH)-independent step. The other known route for this conversion is the two-step GSH-dependent pathway catalysed by EC 4.4.1.5 (lactoylglutathione lyase) and EC 3.1.2.6 (hydroxyacylglutathione hydrolase).
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This record set is specific for:
UNIPROT: Q9ZV19
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
hsp31, glyoxalase iii, dj-1a, dj-1b, glutathione-independent glyoxalase, glutathione-independent glyoxalase iii, hsp3101, hsp3102, hsp31p glyoxalase iii, heat shock protein 31, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
protein DJ-1 homolog E
UniProt
glyoxylase III
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-lactate hydro-lyase
The enzyme, described from the fungi Candida albicans and Schizosaccharomyces pombe, converts 2-oxopropanal to (R)-lactate in a single glutathione (GSH)-independent step. The other known route for this conversion is the two-step GSH-dependent pathway catalysed by EC 4.4.1.5 (lactoylglutathione lyase) and EC 3.1.2.6 (hydroxyacylglutathione hydrolase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxopropanal + H2O
(R)-lactate
show the reaction diagram
-
-
-
?
glyoxal + H2O
acetate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-oxopropanal + H2O
(R)-lactate
show the reaction diagram
-
-
-
?
glyoxal + H2O
acetate
show the reaction diagram
-
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0062
substrate methylglyoxal, pH 6.8, 37°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the DJ-1/PfpI superfamily. Unlike the homologues from other species, all the Arabidopsis thaliana genes have two tandem domains, which may have been created by gene duplication. The six AtDJ-1 proteins (a-f) are characterized
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DJ1E_ARATH
398
0
42730
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
132000
gel filtration, recombinant enzyme
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
3 * 42700, about, sequence calculation
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene DJ1E, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kwon, K.; Choi, D.; Hyun, J.K.; Jung, H.S.; Baek, K.; Park, C.
Novel glyoxalases from Arabidopsis thaliana
FEBS J.
280
3328-3339
2013
Arabidopsis thaliana (Q8VY09), Arabidopsis thaliana (Q9FPF0), Arabidopsis thaliana (Q9M1G8), Arabidopsis thaliana (Q9MAH3), Arabidopsis thaliana (Q9ZV19), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (Q8VY09), Arabidopsis thaliana Col-0 (Q9FPF0), Arabidopsis thaliana Col-0 (Q9M1G8), Arabidopsis thaliana Col-0 (Q9MAH3), Arabidopsis thaliana Col-0 (Q9ZV19)
Manually annotated by BRENDA team