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Information on EC 4.2.1.130 - D-lactate dehydratase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q04432

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.130 D-lactate dehydratase
IUBMB Comments
The enzyme, described from the fungi Candida albicans and Schizosaccharomyces pombe, converts 2-oxopropanal to (R)-lactate in a single glutathione (GSH)-independent step. The other known route for this conversion is the two-step GSH-dependent pathway catalysed by EC 4.4.1.5 (lactoylglutathione lyase) and EC 3.1.2.6 (hydroxyacylglutathione hydrolase).
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Saccharomyces cerevisiae
UNIPROT: Q04432
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
hsp31, glyoxalase iii, dj-1a, dj-1b, glutathione-independent glyoxalase, hsp3101, hsp3102, glutathione-independent glyoxalase iii, dj-1 glyoxalase, hsp31p glyoxalase iii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glutathione-independent glyoxalase
-
glutathione-independent glyoxalase III
-
GSH-independent glyoxalase III
-
heat shock protein 31
-
Hsp31p glyoxalase III
-
glyoxylase III
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-lactate hydro-lyase
The enzyme, described from the fungi Candida albicans and Schizosaccharomyces pombe, converts 2-oxopropanal to (R)-lactate in a single glutathione (GSH)-independent step. The other known route for this conversion is the two-step GSH-dependent pathway catalysed by EC 4.4.1.5 (lactoylglutathione lyase) and EC 3.1.2.6 (hydroxyacylglutathione hydrolase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-lactate
2-oxopropanal + H2O
show the reaction diagram
acetate
2-oxoethanal + H2O
show the reaction diagram
-
-
-
?
methylglyoxal + H2O
(R)-lactate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-lactate
2-oxopropanal + H2O
show the reaction diagram
glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH
-
-
?
acetate
2-oxoethanal + H2O
show the reaction diagram
-
-
-
?
methylglyoxal + H2O
(R)-lactate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
the enzyme shows GSH-independent glyoxalase activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
-
additional information
the enzyme shows GSH-independent glyoxalase activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
methylglyoxal
recombinant ScHsp31, pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.25
methylglyoxal
recombinant ScHsp31, pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.85
methylglyoxal
recombinant ScHsp31, pH and temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
the absence of the HSP31 gene sensitizes cells to oxidative, osmotic and thermal stresses, to potentially toxic products of glycolysis, such as methylglyoxal and acetic acid, and to the diauxic shift
metabolism
overexpression of Hsp31 in tobacco imparts robust stress tolerance against diverse biotic stress inducers such as viruses, bacteria and fungi, in addition to tolerance against a range of abiotic stress inducers
physiological function
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
overexpression of Saccharomyces cerevisiae ScHSP31 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Nicotiana tabacum cv. Wisconsin. Overexpression of Hsp31 in tobacco imparts robust stress tolerance against diverse biotic stress inducers such as viruses, bacteria and fungi, in addition to tolerance against a range of abiotic stress inducers. During stress, Hsp31 is targeted to mitochondria and induces expression of key stress-related genes
gene ScHSP31 encoding Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells
gene ScHSP31, expression in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview. Overexpression of ScHSP31 can confer methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1-deletion of Schizosaccharomyces pombe
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
strong induction of this gene is caused by high concentrations of ethanol and other products of glycolysis. HSP31 gene expression is controlled by multiple transcription factors, including Yap1p, Cad1p, Msn2p, Msn4p, Haa1p and Hsf1p. These transcription factors mediate the HSP31 promoter responses to oxidative, osmotic and thermal stresses, to potentially toxic products of glycolysis, such as methylglyoxal and acetic acid, and to the diauxic shift
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
Hsp31 is a tool to engineer plants against both biotic and abiotic stresses
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhao, Q.; Su, Y.; Wang, Z.; Chen, C.; Wu, T.; Huang, Y.
Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe
BMC Evol. Biol.
14
86
2014
Saccharomyces cerevisiae (Q04432), Schizosaccharomyces pombe, Schizosaccharomyces pombe (O74914), Schizosaccharomyces pombe (Q09675), Schizosaccharomyces pombe (Q10356), Schizosaccharomyces pombe 972 (O74914)
Manually annotated by BRENDA team
Natkanska, U.; Skoneczna, A.; Sienko, M.; Skoneczny, M.
The budding yeast orthologue of Parkinsons disease-associated DJ-1 is a multi-stress response protein protecting cells against toxic glycolytic products
Biochim. Biophys. Acta
1864
39-50
2017
Saccharomyces cerevisiae (Q04432), Saccharomyces cerevisiae ATCC 204508 (Q04432)
-
Manually annotated by BRENDA team
Melvin, P.; Bankapalli, K.; DSilva, P.; Shivaprasad, P.V.
Methylglyoxal detoxification by a DJ-1 family protein provides dual abiotic and biotic stress tolerance in transgenic plants
Plant Mol. Biol.
94
381-397
2017
Saccharomyces cerevisiae (Q04432), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q04432)
Manually annotated by BRENDA team