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Information on EC 4.2.1.121 - colneleate synthase and Organism(s) Nicotiana tabacum and UniProt Accession Q8W2N5

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.121 colneleate synthase
IUBMB Comments
A heme-thiolate protein (P-450) . It catalyses the selective removal of pro-R hydrogen at C-8 in the biosynthesis of colneleic acid . It forms also (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid (i.e. colnelenate) from (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate. The corresponding 13-hydroperoxides are poor substrates [1,3]. The divinyl ethers colneleate and colnelenate have antimicrobial activity.
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This record set is specific for:
Nicotiana tabacum
UNIPROT: Q8W2N5
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Word Map
The taxonomic range for the selected organisms is: Nicotiana tabacum
The enzyme appears in selected viruses and cellular organisms
Synonyms
divinyl ether synthase, ledes, ntdes1, 9-des, cyp74d, dve synthase, 9-divinyl ether synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9-divinyl ether synthase
-
-
DVE synthase
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(8E)-9-[(1E,3E)-nona-1,3-dien-1-yloxy]non-8-enoate synthase
A heme-thiolate protein (P-450) [2]. It catalyses the selective removal of pro-R hydrogen at C-8 in the biosynthesis of colneleic acid [4]. It forms also (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid (i.e. colnelenate) from (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate. The corresponding 13-hydroperoxides are poor substrates [1,3]. The divinyl ethers colneleate and colnelenate have antimicrobial activity.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
(8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoic acid + H2O
show the reaction diagram
the corresponding 13-hydroperoxide is a poor substrate
i.e. colneleate, characterization of the product
-
?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
(8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid + H2O
show the reaction diagram
the corresponding 13-hydroperoxide is a poor substrate
i.e. colnelenate
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
9-lipoxygenase and divinyl ether synthase act together and form divinyl ethers in response to pathogen attack
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DES_TOBAC
478
0
54079
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant glutathione S-transferase fusion protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a glutathione S-transferase fusion protein
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
DES1 gene expression is not detectable in healthy or wounded stems, leaves and roots of tobacco plants
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ethylene and methyl jasmonate positively stimulate the transcription of DES1
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fammartino, A.; Cardinale, F.; Gbel, C.; Mene-Saffrane, L.; Fournier, J.; Feussner, I.; Esquerre-Tugaye, M.T.
Characterization of a divinyl ether biosynthetic pathway specifically associated with pathogenesis in tobacco
Plant Physiol.
143
378-388
2007
Nicotiana tabacum (Q8W2N5), Nicotiana tabacum
Manually annotated by BRENDA team
Fammartino, A.; Verdaguer, B.; Fournier, J.; Tamietti, G.; Carbonne, F.; Esquerre-Tugaye, M.T.; Cardinale, F.
Coordinated transcriptional regulation of the divinyl ether biosynthetic genes in tobacco by signal molecules related to defense
Plant Physiol. Biochem.
48
225-231
2010
Nicotiana tabacum
Manually annotated by BRENDA team