Catalyses the dehydration of (2S,3S)-2-methylcitrate, forming the trans isomer of 2-methyl-aconitate (unlike EC 4.2.1.79, which forms only the cis isomer). Part of a propionate degradation pathway. The enzyme from Shewanella oneidensis can also accept citrate and cis-aconitate, but activity with (2S,3S)-2-methylcitrate was approximately 2.5-fold higher. 2-methylisocitrate and isocitrate were not substrates . An iron-sulfur protein.
The expected taxonomic range for this enzyme is: Shewanella oneidensis
Catalyses the dehydration of (2S,3S)-2-methylcitrate, forming the trans isomer of 2-methyl-aconitate (unlike EC 4.2.1.79, which forms only the cis isomer). Part of a propionate degradation pathway. The enzyme from Shewanella oneidensis can also accept citrate and cis-aconitate, but activity with (2S,3S)-2-methylcitrate was approximately 2.5-fold higher. 2-methylisocitrate and isocitrate were not substrates [1]. An iron-sulfur protein.
the two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD from Salmonella enterica, which encodes 2-methylcitrate dehydratase, PrpD /EC 4.2.1.79. SoAcnD and SePrpD synthesize different methylaconitate isomers. The SoPrpF protein isomerizes the product of the AcnD reaction into the PrpD product (2-MCA), known substrate of the housekeeping aconitase. It is likely that 4-methylaconitate is the product of the AcnD enzyme. PrpF (SoPrpF) protein isomerizes the product of the SoAcnD dehydratase (putatively 4-methyl-cis-aconitate (4-MCA)) to 2-methyl-cis-aconitate (2-MCA). Thus,the role of SoPrpF in the 2-methylcitrate cycle (2-MCC) is to change the stereochemistry of the SoAcnD reaction product. AcnD and PrpF activities are necessary and sufficient to compensate for the lack of PrpD during growth with propionate of the strain of Salmonella enterica. 2-Methylcitrate is dehydrated by AcnD to 4-methyl-cis-aconitate, which is then isomerized to 2-methyl-cis-aconitate by PrpF, and rehydrated to 2-methylisocitrate by aconitases A and B
the enzyme is involved in catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. The 2-methylcitric acid cycle is a common route of propionate catabolism in microorganisms
gene acnD, the sequence of prp genes is prpR-prpB-prpC-acnD-prpF, functional recombinant coexpression of the genes acnD and prpF from Shewanella oneidensis in Salmonella enterica strain DELTAprpD, recombinant expression of His-tagged enzyme from gene acnD in Escherichia coli strain BL21(DE3)
The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo
The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle
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