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Information on EC 4.2.1.100 - cyclohexa-1,5-dienecarbonyl-CoA hydratase for references in articles please use BRENDA:EC4.2.1.100Word Map on EC 4.2.1.100
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
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cyclohexa-1,5-dienecarbonyl-CoA hydratase
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6-hydroxycyclohex-1-ene-1-carbonyl-CoA = cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
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benzoyl-CoA degradation II (anaerobic)
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crotonate fermentation (to acetate and cyclohexane carboxylate)
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benzoyl-CoA degradation
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Microbial metabolism in diverse environments
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6-hydroxycyclohex-1-enecarbonyl-CoA hydro-lyase (cyclohexa-1,5-dienecarbonyl-CoA-forming)
Forms part of the anaerobic benzoate degradation pathway, which also includes EC 1.3.8.6 [glutaryl-CoA dehydrogenase (ETF)], EC 1.3.7.8 (benzoyl-CoA reductase) and EC 4.2.1.55 (3-hydroyxbutyryl-CoA dehydratase).
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cyclohexa-1,5-diene-1-carbonyl-CoA hydratase
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Cyclohexa-1,5-diene-1-carboxyl-CoA hydratase
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EC 4.2.1.102
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formerly
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dienoyl-CoA hydratase
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dienoyl-CoA hydratase
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dienoyl-CoA hydratase
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dienoyl-CoA hydratase
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Ferp_1031
gene name
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strain DSMZ 2059, grown with benzoate as substrate
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UniProt
brenda
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UniProt
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strain DSMZ 7210, grown with benzoate as substrate
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grown with benzoate as substrate
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brenda
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formerly known as Pseudomonas sp. strains K 172 and KB 740
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brenda
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brenda
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UniProt
brenda
denitrifying bacterium, inducible enzyme
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brenda
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metabolism
the enzyme is involved in benzoyl-coenzyme A degradation
metabolism
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the enzyme is involved in benzoyl-coenzyme A degradation
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cyclohex-1-ene-1-carbonyl-CoA + H2O
2-hydroxycyclohexane-1-carbonyl-CoA
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
cyclohexa-1,5-dienecarbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
trans-1,4 water addition, stereochemistry of 6-hydroxycyclohex-1-ene-1-carboxyl-CoA by NMR analysis
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?
cyclohexa-2,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-2-ene-1-carbonyl-CoA
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only when dithionite is used as an artificial electron donor
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
the enzyme is involved in benzoyl-coenzyme A degradation
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
the enzyme is involved in benzoyl-coenzyme A degradation
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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involved in the benzoyl-CoA pathway
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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involved in the benzoyl-CoA pathway
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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involved in the benzoyl-CoA pathway
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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?
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cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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involved in the benzoyl-CoA pathway
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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involved in the benzoyl-CoA pathway
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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involved in the benzoyl-CoA pathway
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r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
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?
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0.05
6-hydroxycyclohex-1-enecarbonyl-CoA
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0.03 - 60
cyclohexa-1,5-diene-1-carbonyl-CoA
0.03
cyclohexa-1,5-diene-1-carbonyl-CoA
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pH 7.5, 30°C
0.085
cyclohexa-1,5-diene-1-carbonyl-CoA
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pH 7.5, 30°C
60
cyclohexa-1,5-diene-1-carbonyl-CoA
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51 - 280
cyclohexa-1,5-diene-1-carbonyl-CoA
51
cyclohexa-1,5-diene-1-carbonyl-CoA
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175
cyclohexa-1,5-diene-1-carbonyl-CoA
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pH 7.5, 30°C
280
cyclohexa-1,5-diene-1-carbonyl-CoA
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pH 7.5, 30°C
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1.8
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cell extract, pH 7.5, 30°C
2
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cell extract, pH 7.5, 30°C
2.5
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cell extract, pH 7.5, 30°C
31.7
85°C, pH 7.0, highly enriched enzyme
110
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reversible reaction with 6-hydroxycyclohex-1-ene-1-carboxyl-CoA as substrat
350
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purified enzyme, hydration reaction, pH 7.5, 30°C
550
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purified enzyme, hydration reaction, pH 7.5, 30°C
730
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purified enzyme, dehydration reaction, pH 7.5, 30°C
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brenda
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28000
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alpha2, 2 * 28000, SDS-PAGE
29900
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4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration
30400
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2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis; 3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration
70000
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Ferguson blot analysis
30000
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2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis; 3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration
30000
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4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration
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tetramer
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4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration
trimer
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3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration
dimer
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2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis
dimer
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alpha2, 2 * 28000, SDS-PAGE
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DEAE-Sepharose, hydroxyapatite, Mono-Q, Blue-Sepharose
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recombinant protein using His-tag
recombinant protein using His-tag
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recombinant protein using His-tag
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expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells
expression in Escherichia coli
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells
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expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells
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DCH_THAAR
258
27882
Swiss-Prot
A0A2H6H921_9BACT
254
27140
TrEMBL
Q1IS86_KORVE
Koribacter versatilis (strain Ellin345)
265
29003
TrEMBL
A0A1V5FAM9_9PROT
258
27696
TrEMBL
A0A1F2PV18_RHOER
255
26848
TrEMBL
A0A2H6HFW0_9BACT
254
26743
TrEMBL
A0A1V5CJS6_9DELT
226
24922
TrEMBL
S0G4H5_9DELT
256
28122
TrEMBL
A0A1J5R2S3_9ZZZZ
257
27994
TrEMBL
A0A0C3MHU7_9RHOO
258
27696
TrEMBL
A0A2H5W690_9BACT
253
28544
TrEMBL
A0A1V5BXR7_9FIRM
254
27498
TrEMBL
A0A0P1J5R1_9RHOB
254
26768
TrEMBL
A0A1V5BIF6_9FIRM
254
27706
TrEMBL
A0A0P1H5X9_9RHOB
255
26680
TrEMBL
A0A0T9KPU0_MYCTX
254
26464
TrEMBL
A0A2X0U6R1_9ACTN
254
27305
TrEMBL
K4Q6J5_9RHOO
256
27704
TrEMBL
A0A1Q9NP01_9ARCH
255
28405
TrEMBL
A0A0B6X3L7_9BACT
258
28704
TrEMBL
A0A0P1FZ99_9RHOB
259
27631
TrEMBL
I4C3Q1_DESTA
Desulfomonile tiedjei (strain ATCC 49306 / DSM 6799 / DCB-1)
259
28642
TrEMBL
A0A2U3LFX6_9DELT
256
28085
TrEMBL
A0A1V2MUV1_9ACTN
257
26478
TrEMBL
A0A1V4W3W0_9FIRM
254
27498
TrEMBL
A0A2R8CDE9_9RHOB
255
27137
TrEMBL
G3C968_9ARCH
271
29189
TrEMBL
A0A1V5E2K0_9DELT
256
27821
TrEMBL
A0A2H6E9F4_9BACT
257
28592
TrEMBL
A0A0U5MEE2_9PROT
259
27647
TrEMBL
D3RXI0_FERPA
Ferroglobus placidus (strain DSM 10642 / AEDII12DO)
252
28129
TrEMBL
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Harwood, C.S.; Gibson, J.
Shedding light on anaerobic benzene ring degradation: a process unique to prokaryotes?
J. Bacteriol.
179
301-309
1997
Azoarcus evansii, Thauera aromatica
brenda
Koch, J.; Eisenreich, W.; Bacher, A.; Fuchs, G.
Products of enzymatic reduction of benzoyl-CoA, a key reaction in anaerobic aromatic metabolism
Eur. J. Biochem.
211
649-661
1993
Azoarcus evansii
brenda
Boll, M.; Laempe, D.; Eisenreich, W.; Bachers, A.; Mittelberger, T.; Heinze, J.; Fuchs, G.
Nonaromatic products from anoxic conversion of benzoyl-CoA with benzoyl-CoA reductase and cyclohexa-1,5-diene-1-carbonyl-CoA hydratase
J. Biol. Chem.
275
21889-21895
2000
Thauera aromatica
brenda
Lampe, D.; Eisenreich, W.; Bacher, A.; Fuchs, G.
Cyclohexa-1,5-diene-1-carboxyl-CoA hydratase, an enzyme involved in anaerobic metabolism of benzoyl-CoA in the denitrifying bacterium Thauera aromatica
Eur. J. Biochem.
255
618-627
1998
Thauera aromatica
brenda
Peters, F.; Shinoda, Y.; McInerney, M.J.; Boll, M.
Cyclohexa-1,5-diene-1-carbonyl-coenzyme A (CoA) hydratases of Geobacter metallireducens and Syntrophus aciditrophicus: Evidence for a common benzoyl-CoA degradation pathway in facultative and strict anaerobes
J. Bacteriol.
189
1055-1060
2007
Desulfococcus multivorans, Geobacter metallireducens, Syntrophus aciditrophicus
brenda
Thiele, B.; Rieder, O.; Golding, B.T.; Mueller, M.; Boll, M.
Mechanism of enzymatic Birch reduction: stereochemical course and exchange reactions of benzoyl-CoA reductase
J. Am. Chem. Soc.
130
14050-14051
2008
Thauera aromatica (o87873)
brenda
Schmid, G.; Rene, S.B.; Boll, M.
Enzymes of the benzoyl-coenzyme A degradation pathway in the hyperthermophilic archaeon Ferroglobus placidus
Environ. Microbiol.
17
3289-3300
2015
Ferroglobus placidus (D3RXI0), Ferroglobus placidus DSM 10642 (D3RXI0)
brenda
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