Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 4.2.1.1 - carbonic anhydrase and Organism(s) Helicobacter pylori and UniProt Accession O24855

for references in articles please use BRENDA:EC4.2.1.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.1 carbonic anhydrase
IUBMB Comments
The enzyme catalyses the reversible hydration of gaseous CO2 to carbonic acid, which dissociates to give hydrogencarbonate above neutral pH. It is widespread and found in archaea, bacteria, and eukaryotes. Three distinct classes exist, and appear to have evolved independently. Contains zinc.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Helicobacter pylori
UNIPROT: O24855
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Helicobacter pylori
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide
H2CO3
=
CO2
+
H2O
=
+
Synonyms
carbonic anhydrase, ca ix, ca ii, hca ii, hca i, carbonic anhydrase ix, carbonic anhydrase ii, hca ix, ca iv, anhydrase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-carbonic anhydrase
-
-
anhydrase
-
-
-
-
CA
-
-
-
-
CA-IX
-
-
-
-
CA-VA
-
-
-
-
CA-VB
-
-
-
-
CA-VI
-
-
-
-
CA-VII
-
-
-
-
CA-XII
-
-
-
-
CA-XIV
-
-
-
-
CA1
-
-
-
-
CA2
-
-
-
-
CAIX
-
-
-
-
carbonate anhydrase
-
-
-
-
Carbonate dehydratase
-
-
-
-
Carbonate dehydratase IX
-
-
-
-
Carbonate dehydratase VA
-
-
-
-
Carbonate dehydratase VB
-
-
-
-
Carbonate dehydratase VI
-
-
-
-
Carbonate dehydratase VII
-
-
-
-
Carbonate dehydratase XII
-
-
-
-
Carbonate dehydratase XIV
-
-
-
-
carbonic acid anhydrase
-
-
-
-
carbonic anhydrase
-
-
-
-
carbonic dehydratase
-
-
-
-
carboxyanhydrase
-
-
-
-
dehydratase, carbonate
-
-
-
-
Membrane antigen MN
-
-
-
-
P54/58N
-
-
-
-
pMW1
-
-
-
-
RCC-associated antigen G250
-
-
-
-
Renal cell carcinoma-associated antigen G250
-
-
-
-
Salivary carbonic anhydrase
-
-
-
-
Secreted carbonic anhydrase
-
-
-
-
Tumor antigen HOM-RCC-3.1.3
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
addition
-
-
-
-
hydration
-
-
dehydration
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
carbonic acid hydro-lyase (carbon-dioxide-forming)
The enzyme catalyses the reversible hydration of gaseous CO2 to carbonic acid, which dissociates to give hydrogencarbonate above neutral pH. It is widespread and found in archaea, bacteria, and eukaryotes. Three distinct classes exist, and appear to have evolved independently. Contains zinc.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-03-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl acetate + H2O
2-nitrophenol + acetate
show the reaction diagram
-
-
-
r
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
-
-
r
CO2 + H2O
H2CO3
show the reaction diagram
H2CO3
CO2 + H2O
show the reaction diagram
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CO2 + H2O
H2CO3
show the reaction diagram
-
periplasmic alpha-carbonic anhydrase activity of Helicobacter pylori is essential for acid acclimation
-
-
?
H2CO3
CO2 + H2O
show the reaction diagram
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(3-methoxypropyl)-4-(methylamino)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
isoenzyme hpalphaCA
4,5-dichloro-benzene-1,3-disulfonamide
isoenzyme hpalphaCA, i.e. daranide
6-chloro-4-amino-benzene-1,3-disulfonamide
isoenzyme hpalphaCA
6-methyl-4-(methylamino)-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
isoenzyme hpalphaCA
6-trifluoromethyl-4-aminobenzene-1,3-disulfonamide
isoenzyme hpalphaCA
acetazolamide
isoenzyme hpalphaCA
ethoxzolamide
isoenzyme hpalphaCA
N-[5-(aminosulfonyl)-3-methyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
isoenzyme hpalphaCA
topiramate
isoenzyme hpalphaCA
2-(3-methoxypropyl)-4-(methylamino)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
isoenzyme hpbetaCA
2-acetylamido-1,3,4-thiadiazole-5-sulfonamide
-
acetazolamide
4,5-dichloro-benzene-1,3-disulfonamide
isoenzyme hpbetaCA, i.e. daranide
6-chloro-4-amino-benzene-1,3-disulfonamide
isoenzyme hpbetaCA
6-methyl-4-(methylamino)-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
isoenzyme hpbetaCA
6-trifluoromethyl-4-aminobenzene-1,3-disulfonamide
isoenzyme hpbetaCA
acetazolamide
isoenzyme hpbetaCA
ethoxzolamide
isoenzyme hpbetaCA
N-[5-(aminosulfonyl)-3-methyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
isoenzyme hpbetaCA
thiocyanate
-
-
topiramate
isoenzyme hpbetaCA
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
CO2
-
pH 8.9, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
240000
CO2
-
pH 8.9, 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00021
2-(3-methoxypropyl)-4-(methylamino)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
isoenzyme hpalphaCA
0.000378
4,5-dichloro-benzene-1,3-disulfonamide
isoenzyme hpalphaCA, i.e. daranide
0.000049
6-chloro-4-amino-benzene-1,3-disulfonamide
isoenzyme hpalphaCA
0.00436
6-methyl-4-(methylamino)-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
isoenzyme hpalphaCA
0.000412
6-trifluoromethyl-4-aminobenzene-1,3-disulfonamide
isoenzyme hpalphaCA
0.000021
acetazolamide
isoenzyme hpalphaCA
0.000193
ethoxzolamide
isoenzyme hpalphaCA
0.000225
N-[5-(aminosulfonyl)-3-methyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
isoenzyme hpalphaCA
0.000172
topiramate
isoenzyme hpalphaCA
0.000128
2-(3-methoxypropyl)-4-(methylamino)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
isoenzyme hpbetaCA
0.000105
4,5-dichloro-benzene-1,3-disulfonamide
isoenzyme hpbetaCA, i.e. daranide
0.00064
6-chloro-4-amino-benzene-1,3-disulfonamide
isoenzyme hpbetaCA
0.000073
6-methyl-4-(methylamino)-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
isoenzyme hpbetaCA
0.000973
6-trifluoromethyl-4-aminobenzene-1,3-disulfonamide
isoenzyme hpbetaCA
0.00004
acetazolamide
isoenzyme hpbetaCA
0.000033
ethoxzolamide
isoenzyme hpbetaCA
0.000176
N-[5-(aminosulfonyl)-3-methyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
isoenzyme hpbetaCA
2.8
thiocyanate
-
pH 7.5, 25°C
0.000032
topiramate
isoenzyme hpbetaCA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
-
calculated from amino acid composition
9
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28280
-
amino acid sequence
29000
-
SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme appears to denature with a midpoint of a structural transition near 2.1 M denaturant guanidine hydrochloride
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha-carbonic anhydrase gene cloned and sequenced, truncated gene expressed in Escherichia coli BL21(DE3)
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chirica, L.C.; Elleby, B.; Lindskog, S.
Cloning, expression and some properties of alpha-carbonic anhydrase from Helicobacter pylori
Biochim. Biophys. Acta
1544
55-63
2001
Helicobacter pylori
Manually annotated by BRENDA team
Marcus, E.A.; Moshfegh, A.P.; Sachs, G.; Scott, D.R.
The periplasmic alpha-carbonic anhydrase activity of Helicobacter pylori is essential for acid acclimation
J. Bacteriol.
187
729-738
2005
Helicobacter pylori
Manually annotated by BRENDA team
Alterio, V.; De Simone, G.; Monti, S.M.; Scozzafava, A.; Supuran, C.T.
Carbonic anhydrase inhibitors: inhibition of human, bacterial, and archaeal isozymes with benzene-1,3-disulfonamides - solution and crystallographic studies
Bioorg. Med. Chem. Lett.
17
4201-4207
2007
Helicobacter pylori (O24855), Helicobacter pylori (Q1CVF1), Homo sapiens (O43570), Homo sapiens (P00915), Homo sapiens (P00918), Homo sapiens (P07451), Homo sapiens (P22748), Homo sapiens (P23280), Homo sapiens (P35218), Homo sapiens (P43166), Homo sapiens (Q16790), Homo sapiens (Q9ULX7), Homo sapiens (Q9Y2D0), Homo sapiens, Methanosarcina thermophila (P40881), Mus musculus (Q9D6N1)
Manually annotated by BRENDA team