The enzymes from the bacteria Pseudomonas aeruginosa and Xenorhabdus nematophila are involved in the biosynthesis of paerucumarin and rhabduscin, respectively. According to the proposed mechanism, the enzyme forms an imine intermediate composed of linked L-tyrosine and D-ribulose 5-phosphate, followed by loss of the phosphate group and formation of a beta-keto imine and keto-enol tautomerization. This is followed by a C-C bond cleavage, the release of hydroxyacetone, and a retro aldol type reaction that releases formaldehyde and forms the final product . cf. EC 4.1.99.25, L-tryptophan isonitrile synthase.
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The enzyme appears in viruses and cellular organisms
The enzymes from the bacteria Pseudomonas aeruginosa and Xenorhabdus nematophila are involved in the biosynthesis of paerucumarin and rhabduscin, respectively. According to the proposed mechanism, the enzyme forms an imine intermediate composed of linked L-tyrosine and D-ribulose 5-phosphate, followed by loss of the phosphate group and formation of a beta-keto imine and keto-enol tautomerization. This is followed by a C-C bond cleavage, the release of hydroxyacetone, and a retro aldol type reaction that releases formaldehyde and forms the final product [3]. cf. EC 4.1.99.25, L-tryptophan isonitrile synthase.
according to the proposed mechanism, the enzyme forms an imine intermediate composed of linked L-tyrosine and D-ribulose 5-phosphate, followed by loss of the phosphate group and formation of a beta-keto imine and keto-enol tautomerization. This is followed by a C-C bond cleavage, the release of hydroxyacetone, and a retro aldol type reaction that releases formaldehyde and forms the final product
pvcA is required for biofilm formation by MPAO1. The pvcA-D gene cluster constitutes the pvc operon, and pvc influences biofilm development by regulating the expression of cupB and cupC. The pvc operon mediates its effect on the cupB and cupC genes through the secondary metabolite paerucumarin
the enzyme is involved in the biosynthesis of paerucumarin. The pvcA-D operon enhances the expression of the Pseudomonas aeruginosa fimbrial chaperone/usher pathway (cup) genes and this effect is mediated by paerucumarin
pvcA is required for biofilm formation by MPAO1. The pvcA-D gene cluster constitutes the pvc operon, and pvc influences biofilm development by regulating the expression of cupB and cupC. The pvc operon mediates its effect on the cupB and cupC genes through the secondary metabolite paerucumarin
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are grown at 20°C by hanging drop vapor diffusion using a 1:1 mixture of protein and precipitant containing 41-45% PEG 1000, 35-50 mM KH2PO4, and 100 mM Tris (pH 9.0). The structure of PvcA demonstrates an enzyme architecture that is built upon a Rossmann fold
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History
Enzyme Nomenclature
4.1.99.24 L-tyrosine isonitrile synthase
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