Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-amino-2-(4-hydroxyphenyl)-ethyl phosphinic acid + H2O
phenol + ?
-
-
-
?
2,5-difluorotyrosine + H2O
2,5-difluorophenol + pyruvate + NH3
-
-
-
?
2,6-difluorotyrosine + H2O
2,6-difluorophenol + pyruvate + NH3
-
-
-
?
2-bromo-L-Tyr + H2O
2-bromophenol + pyruvate + NH3
-
-
-
r
2-bromophenol + pyruvate + NH3
2-bromo-L-Tyr + H2O
-
-
-
r
2-chloro-L-Tyr + H2O
2-chlorophenol + pyruvate + NH3
2-chlorophenol + pyruvate + NH3
2-chloro-L-Tyr + H2O
-
-
-
r
2-fluoro-L-Tyr + H2O
2-fluorophenol + pyruvate + NH3
2-fluorophenol + pyruvate + NH3
2-fluoro-L-Tyr + H2O
-
-
-
r
2-fluorotyrosine + H2O
2-fluorophenol + pyruvate + NH3
-
-
-
?
2-methoxy-L-Tyr + H2O
2-methoxyphenol + pyruvate + NH3
-
-
-
r
2-methoxyphenol + pyruvate + NH3
2-methoxy-L-Tyr + H2O
-
-
-
r
2-methyl-L-Tyr + H2O
2-methylphenol + pyruvate + NH3
-
-
-
r
2-methylphenol + pyruvate + NH3
2-methyl-L-Tyr + H2O
-
-
-
r
3,4-dihydroxyphenyl-L-Ala + H2O
pyrocatechol + NH3 + pyruvate
3,4-dihydroxyphenyl-L-alanine
?
Symbiobacterium sp.
-
-
-
?
3,4-dihydroxyphenyl-L-alanine + H2O
pyrocatechol + NH3 + pyruvate
-
-
-
-
?
3,5-difluorotyrosine + H2O
3,5-difluorophenol + pyruvate + NH3
-
-
-
?
3-bromo-L-Tyr + H2O
3-bromophenol + pyruvate + NH3
-
-
-
r
3-bromo-L-tyrosine + H2O
3-bromophenol + pyruvate + NH3
-
-
-
-
?
3-bromophenol + pyruvate + NH3
3-bromo-L-Tyr + H2O
-
-
-
r
3-chloro-L-Tyr + H2O
3-chlorophenol + pyruvate + NH3
-
-
-
r
3-chloro-L-tyrosine + H2O
3-chlorophenol + pyruvate + NH3
-
-
-
-
?
3-chloro-L-tyrosine + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
3-chlorophenol + pyruvate + NH3
3-chloro-L-Tyr + H2O
-
-
-
r
3-chlorotyrosine + H2O
3-chlorophenol + pyruvate + NH3
-
-
-
?
3-fluoro-L-Tyr + H2O
3-fluorophenol + pyruvate + NH3
3-fluoro-L-tyrosine
3-fluorophenol + pyruvate + NH3
-
-
-
?
3-fluoro-L-tyrosine + H2O
3-fluoro-phenol + pyruvate + NH3
-
-
?
3-fluoro-L-tyrosine + H2O
3-fluorophenol + pyruvate + NH3
3-fluoro-L-tyrosine + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
3-fluorophenol + pyruvate + NH3
3-fluoro-L-Tyr + H2O
-
-
-
r
3-fluorotyrosine + H2O
3-fluorophenol + pyruvate + NH3
-
-
-
?
3-iodo-L-tyrosine + H2O
3-iodophenol + pyruvate + NH3
-
-
-
-
?
3-methoxy-L-tyrosine + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
3-methyl-L-Tyr + H2O
3-methylphenol + pyruvate + NH3
-
-
-
r
3-methyl-L-tyrosine + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
3-methylphenol + pyruvate + NH3
3-methyl-L-Tyr + H2O
-
-
-
r
allothreonine + ?
Gly + ?
-
-
-
?
beta-chloro-L-alanine + H2O
?
beta-chloro-L-alanine + H2O
Cl- + pyruvate + NH3
-
-
-
r
catechol + pyruvate
L-DOPA + H2O
catechol + pyruvate + NH3
3,4-dihydroxyphenyl-L-alanine
catechol + pyruvate + NH3
L-dihydroxyphenylalanine
catechol + pyruvic acid
L-dopa + H2O
-
-
-
-
?
D-serine + H2O
?
Symbiobacterium sp.
-
at 11% the rate of L-tyrosine
-
?
D-tyrosine + H2O
phenol + pyruvate + NH3
L-3-4 dihydroxyphenylalanine + H2O
catechol + pyruvate + NH3
L-Ala + pyridoxal phosphate
pyridoxamine phosphate + keto acid
-
-
-
-
ir
L-alanine + H2O
?
-
wild-type enzyme forms the Ala quinonoid intermediate when incubated with L-Ala
-
-
?
L-Asp + H2O
formate + pyruvate + NH3
L-asparagine + H2O
?
-
-
-
-
?
L-Cys + H2O
? + pyruvate + NH3
L-cysteine + H2O
thiophenol + pyruvate + NH3
12% of the activity with L-tyrosine
-
-
r
L-cystine + H2O
? + pyruvate + NH3
L-DOPA + H2O
pyrocatechol + pyruvate + NH4+
42% of the activity with L-tyrosine
-
-
r
L-m-Tyr + pyridoxal phosphate
pyridoxamine phosphate + keto acid
-
-
-
-
ir
L-methionine + H2O
?
-
-
-
-
?
L-Phe + pyridoxal phosphate
pyridoxamine phosphate + keto acid
-
-
-
-
ir
L-phenylalanine + H2O
?
-
low activity
-
-
?
L-phenylalanine + H2O
? + pyruvate + NH3
7% of the activity with L-tyrosine
-
-
r
L-Ser + phenol
L-Tyr + H2O
L-Ser + pyridoxal phosphate
pyridoxamine phosphate + keto acid
-
-
-
-
ir
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
L-serine + H2O
?
Symbiobacterium sp.
-
-
-
?
L-serine + H2O
pyruvate + NH3
9% of the activity with L-tyrosine
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
L-Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
L-tyrosine + H2O
3,4-dihydroxyphenyl-L-alanine
L-tyrosine + H2O
phenol + pyruvate + NH3
L-valine + H2O
?
-
-
-
-
?
L-valine + H2O
? + pyruvate + NH3
6% of the activity with L-tyrosine
-
-
r
O-benzyl-L-Cys + H2O
?
-
-
-
?
O-benzyl-L-Ser + H2O
?
-
-
-
?
o-chlorophenol + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
o-cresol + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
o-methoxyphenol + H2O
?
-
substrate of mutant M379V, not of the wild-type enzyme
-
-
?
phenol + pyruvate + NH3
L-tyrosine + H2O
-
-
-
r
phenol + pyruvate + NH3
Tyr + H2O
pyrocatechol + pyruvate + NH3
3,4-dihydroxyphenyl-L-Ala + H2O
pyrocatechol + pyruvate + NH4+
L-DOPA + H2O
resorcinol + pyruvate + NH3
2,4-dihydroxyphenyl-L-Ala
-
-
-
?
S-(2-nitrophenyl)-L-cysteine
?
-
-
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
S-(o-nitrophenyl)-L-Cys + H2O
?
-
-
-
-
?
S-(o-nitrophenyl)-L-Cys + H2O
? + pyruvate + NH3
-
-
-
-
?
S-(o-nitrophenyl)-L-cysteine
?
S-(o-nitrophenyl)-L-cysteine + H2O
?
S-benzoyl-L-Cys + H2O
?
-
-
-
-
?
S-benzyl-L-Cys + H2O
? + pyruvate + NH3
S-benzyl-L-cysteine
? + pyruvate + NH3
-
-
-
-
?
S-benzyl-L-cysteine + H2O
thiophenol + pyruvate + NH3
-
-
-
r
S-ethyl-L-cysteine
? + pyruvate + NH3
-
-
-
-
?
S-ethyl-L-cysteine + H2O
?
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH3
-
-
-
r
S-methyl-L-Cys + H2O
? + pyruvate + NH3
S-methyl-L-cysteine
? + pyruvate + NH3
-
-
-
-
?
S-methyl-L-cysteine + H2O
?
Symbiobacterium sp.
-
385% of activity compared to L-tyrosine
-
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH3
-
-
-
r
S-methyl-L-cysteine + H2O
methanthiol + pyruvate + NH3
13% of the activity with L-tyrosine
-
-
r
S-o-nitrophenyl-L-cysteine + H2O
?
-
-
-
-
?
Ser + H2O
? + pyruvate + NH3
Thr + ?
2-oxobutanoate + ?
Tyr + H2O
phenol + pyruvate + NH3
additional information
?
-
2-chloro-L-Tyr + H2O

2-chlorophenol + pyruvate + NH3
-
-
-
r
2-chloro-L-Tyr + H2O
2-chlorophenol + pyruvate + NH3
-
-
-
-
?
2-fluoro-L-Tyr + H2O

2-fluorophenol + pyruvate + NH3
-
-
-
r
2-fluoro-L-Tyr + H2O
2-fluorophenol + pyruvate + NH3
-
-
-
-
?
3,4-dihydroxyphenyl-L-Ala + H2O

pyrocatechol + NH3 + pyruvate
-
-
-
?
3,4-dihydroxyphenyl-L-Ala + H2O
pyrocatechol + NH3 + pyruvate
-
-
-
-
?
3,4-dihydroxyphenyl-L-Ala + H2O
pyrocatechol + NH3 + pyruvate
-
i.e. L-dopa
-
?
3,4-dihydroxyphenyl-L-Ala + H2O
pyrocatechol + NH3 + pyruvate
-
i.e. L-dopa
-
?
3-chloro-L-Ala + H2O

?
-
-
-
-
?
3-chloro-L-Ala + H2O
?
-
-
-
-
?
3-chloro-L-Ala + H2O
?
-
-
-
-
?
3-chloro-L-Ala + H2O
?
-
-
-
-
?
3-fluoro-L-Tyr + H2O

3-fluorophenol + pyruvate + NH3
-
-
-
r
3-fluoro-L-Tyr + H2O
3-fluorophenol + pyruvate + NH3
-
-
-
-
?
3-fluoro-L-Tyr + H2O
3-fluorophenol + pyruvate + NH3
-
-
-
-
?
3-fluoro-L-tyrosine + H2O

3-fluorophenol + pyruvate + NH3
-
-
-
-
?
3-fluoro-L-tyrosine + H2O
3-fluorophenol + pyruvate + NH3
-
-
-
r
3-fluoro-L-tyrosine + H2O
3-fluorophenol + pyruvate + NH3
3-fluoro-L-tyrosine also is a good substrate for the wild-type enzyme
-
-
r
Ala + H2O

?
-
L-Ala
-
-
?
Ala + H2O
?
-
D-Ala
-
-
?
beta-chloro-L-alanine

?
-
-
-
?
beta-chloro-L-alanine
?
-
-
?
beta-chloro-L-alanine + H2O

?
-
-
-
r
beta-chloro-L-alanine + H2O
?
Symbiobacterium sp.
-
500% of activity compared to L-tyrosine
-
?
beta-chloro-L-alanine + H2O
?
-
500% of activity compared to L-tyrosine
-
?
catechol + pyruvate

L-DOPA + H2O
-
-
-
r
catechol + pyruvate
L-DOPA + H2O
-
-
-
r
catechol + pyruvate + NH3

3,4-dihydroxyphenyl-L-alanine
-
TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels
-
-
r
catechol + pyruvate + NH3
3,4-dihydroxyphenyl-L-alanine
-
TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels
-
-
r
catechol + pyruvate + NH3
3,4-dihydroxyphenyl-L-alanine
-
-
-
-
r
catechol + pyruvate + NH3

L-dihydroxyphenylalanine
-
-
-
?
catechol + pyruvate + NH3
L-dihydroxyphenylalanine
-
-
-
-
?
D-tyrosine + H2O

phenol + pyruvate + NH3
Symbiobacterium sp.
-
at 7% the rate of L-tyrosine
-
?
D-tyrosine + H2O
phenol + pyruvate + NH3
-
at 7% the rate of L-tyrosine
-
?
L-2-aminoadipate + H2O

?
-
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
-
-
?
L-2-aminoadipate + H2O
?
-
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
-
-
?
L-3-4 dihydroxyphenylalanine + H2O

catechol + pyruvate + NH3
-
L-dopa
-
-
r
L-3-4 dihydroxyphenylalanine + H2O
catechol + pyruvate + NH3
-
L-DOPA, alpha-beta elimination
-
-
r
L-3-4 dihydroxyphenylalanine + H2O
catechol + pyruvate + NH3
-
L-dopa
-
-
r
L-Asp + H2O

formate + pyruvate + NH3
-
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
-
?
L-Asp + H2O
formate + pyruvate + NH3
-
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
-
?
L-Cys + H2O

? + pyruvate + NH3
-
-
-
?
L-Cys + H2O
? + pyruvate + NH3
-
-
-
?
L-Cys + H2O
? + pyruvate + NH3
-
-
-
?
L-Cys + H2O
? + pyruvate + NH3
-
-
-
-
?
L-cysteine + H2O

?
Symbiobacterium sp.
-
-
-
?
L-cysteine + H2O
?
-
-
-
?
L-cystine + H2O

? + pyruvate + NH3
-
-
-
?
L-cystine + H2O
? + pyruvate + NH3
-
-
-
?
L-Glu + H2O

?
-
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
-
-
?
L-Glu + H2O
?
-
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
-
-
?
L-Phe + H2O

?
-
-
-
-
?
L-Ser + phenol

L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
Pseudomonas pelurida
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
Pseudomonas trifolii
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + phenol
L-Tyr + H2O
-
-
-
?
L-Ser + pyrocatechol

3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
i.e. L-dopa
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
Pseudomonas pelurida
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
Pseudomonas trifolii
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-Ser + pyrocatechol
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
L-tryptophan + H2O

indole + pyruvate + NH3
8% of the activity with L-tyrosine, reaction of EC 4.1.99.1
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
8% of the activity with L-tyrosine, reaction of EC 4.1.99.1
-
-
r
L-tyrosine + H2O

3,4-dihydroxyphenyl-L-alanine
-
TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels
-
-
?
L-tyrosine + H2O
3,4-dihydroxyphenyl-L-alanine
-
TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels
-
-
?
L-tyrosine + H2O

phenol + pyruvate + NH3
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
alpha-beta elimination
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
reversible cleavage of the Cbeta-Cgamma bond of L-tyrosine in the final stage of the beta-elimination, via intermediates: internal aldimine, external aldimine, quinoid, ketoquinoid, alpha-aminoacrylate, and internal aldimine, overview
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
very high substrate specificity of TPL
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
high activity with the wild-type enzyme
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
alpha-beta elimination
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
L-tyrosine is the physiological substrate of enzyme Fn-TPL
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
alpha,beta-elimination of L-tyrosine, best substrate
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
L-tyrosine is the physiological substrate of enzyme Fn-TPL
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
alpha,beta-elimination of L-tyrosine, best substrate
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
r
L-tyrosine + H2O
phenol + pyruvate + NH3
Symbiobacterium sp.
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
?
L-tyrosine + H2O
phenol + pyruvate + NH3
-
-
-
-
r
O-benzoyl-L-Ser + H2O

?
-
-
-
-
?
O-benzoyl-L-Ser + H2O
?
-
-
-
?
phenol + pyruvate + NH3

Tyr + H2O
-
-
-
?
phenol + pyruvate + NH3
Tyr + H2O
-
-
-
r
pyrocatechol + pyruvate + NH3

3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
pyrocatechol + pyruvate + NH3
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
pyrocatechol + pyruvate + NH3
3,4-dihydroxyphenyl-L-Ala + H2O
-
-
-
?
pyrocatechol + pyruvate + NH4+

L-DOPA + H2O
-
-
-
r
pyrocatechol + pyruvate + NH4+
L-DOPA + H2O
-
-
-
r
S-(2-nitrophenyl)-L-cysteine + H2O

2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
r
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
best substrate for the wild-type enzyme
-
-
r
S-(o-nitrophenyl)-L-cysteine

?
-
-
-
?
S-(o-nitrophenyl)-L-cysteine
?
-
-
-
?
S-(o-nitrophenyl)-L-cysteine
?
-
-
?
S-(o-nitrophenyl)-L-cysteine + H2O

?
-
-
-
r
S-(o-nitrophenyl)-L-cysteine + H2O
?
-
-
-
?
S-(o-nitrophenyl)-L-cysteine + H2O
?
Symbiobacterium sp.
-
250% of activity compared to L-tyrosine
-
?
S-(o-nitrophenyl)-L-cysteine + H2O
?
-
250% of activity compared to L-tyrosine
-
?
S-benzyl-L-Cys + H2O

? + pyruvate + NH3
-
-
-
?
S-benzyl-L-Cys + H2O
? + pyruvate + NH3
-
-
-
-
?
S-benzyl-L-Cys + H2O
? + pyruvate + NH3
-
-
-
?
S-benzyl-L-cysteine

?
-
-
-
?
S-benzyl-L-cysteine
?
-
-
?
S-ethyl-L-Cys + H2O

?
-
-
-
-
?
S-ethyl-L-Cys + H2O
?
-
-
-
?
S-ethyl-L-cysteine

?
-
-
-
?
S-ethyl-L-cysteine
?
-
-
?
S-methyl-L-Cys + H2O

? + pyruvate + NH3
-
-
-
-
?
S-methyl-L-Cys + H2O
? + pyruvate + NH3
-
-
-
?
S-methyl-L-Cys + H2O
? + pyruvate + NH3
-
-
-
?
S-methyl-L-Cys + H2O
? + pyruvate + NH3
-
-
-
-
?
S-methyl-L-Cys + H2O
? + pyruvate + NH3
-
-
-
-
?
S-methyl-L-cysteine

?
-
-
-
?
S-methyl-L-cysteine
?
-
-
-
?
S-methyl-L-cysteine
?
-
-
?
Ser + H2O

? + pyruvate + NH3
-
L-Ser
-
-
?
Ser + H2O
? + pyruvate + NH3
-
-
-
-
?
Ser + H2O
? + pyruvate + NH3
-
-
-
?
Ser + H2O
? + pyruvate + NH3
-
L-Ser
-
-
?
Ser + H2O
? + pyruvate + NH3
-
D-Ser
-
-
?
Ser + H2O
? + pyruvate + NH3
-
L-Ser
-
-
?
Thr + ?

2-oxobutanoate + ?
-
alpha,beta-elimination
-
?
Thr + ?
2-oxobutanoate + ?
-
D-Thr
-
?
Thr + ?
2-oxobutanoate + ?
-
L-Thr
-
?
Tyr + H2O

phenol + pyruvate + NH3
Aeromonas phenologenes
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
L-Tyr
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
L-Tyr
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
L-Tyr
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
L-Tyr
-
-
r
Tyr + H2O
phenol + pyruvate + NH3
-
D-Tyr
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
L-Tyr
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
-
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
L-Tyr
-
-
?
Tyr + H2O
phenol + pyruvate + NH3
-
D-Tyr
-
-
?
additional information

?
-
-
catalyzes racemization of L-Ala
-
-
?
additional information
?
-
-
mechanism of alpha-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase
-
-
?
additional information
?
-
-
alanine, arginine, aspartate, cysteine, glutamine, glutamate, glycine, histidine, isoleucine, leucine, lysine, proline, serine, threonine, tryptophan are poor or no substrates, substrate specificity of purified tyrosine phenol lyase, overview
-
-
?
additional information
?
-
-
substrate 3-fluoro-L-tyrosine is converted only to the quinoid reaction intermediate, structure determination of 3-fluoro-L-tyrosine bound to enzyme mutant Y71F with open active site and mutant F448H with closed active site, overview
-
-
?
additional information
?
-
-
substrate specificity with 3-substituted tyrosines, the wild-type enzyme shows a narrow substrate spectrum, overview. Mutant M379V converts several L-tyrosine derivatives to the correspondent products
-
-
?
additional information
?
-
fragment of active site structure of the quinonoid complex of wild-type enzyme TPL with L-alanine, overview
-
-
?
additional information
?
-
of the common S-alkyl-L-cysteine substrates, S-ethyl-L-cysteine has the most favorable kinetic properties with TPL
-
-
?
additional information
?
-
tyrosine phenol-lyase is a pyridoxal 5'-phosphate (PLP)-dependent bacterial enzyme that catalyzes the reversible hydrolytic cleavage of the Cbeta-Cgamma bond of L-Tyr to phenol and ammonium pyruvate. In addition to its physiological substrate L-Tyr, TPL also catalyzes the in vitro beta-elimination of substrates with good leaving groups on the beta-carbon, including L-serine, L-cysteine, S-(2-nitrophenyl)-L-cysteine, O-acetyl-L-serines, and S-alkyl-L-cysteines
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
catalyzes racemization of L-Ala
-
-
?
additional information
?
-
-
inducible enzyme
-
-
?
additional information
?
-
-
adaptive enzyme responsible for cells' growth on media with L-Tyr as the sole source of carbon
-
-
?
additional information
?
-
-
inducible enzyme
-
-
?
additional information
?
-
low activity of Fn-TPL is observed toward natural amino acids including L-serine, L-cysteine, L-phenylalanine, L-valine, L-tryptophan, no activity with L-threonine, L-glutamine, L-alanine, D-alanine, L-arginine, L-leucine, L-histidine, L-aspartic acid, L-methionine, and D-methionine
-
-
?
additional information
?
-
low activity of Fn-TPL is observed toward natural amino acids including L-serine, L-cysteine, L-phenylalanine, L-valine, L-tryptophan, no activity with L-threonine, L-glutamine, L-alanine, D-alanine, L-arginine, L-leucine, L-histidine, L-aspartic acid, L-methionine, and D-methionine
-
-
?
additional information
?
-
-
inducible enzyme
-
-
?
additional information
?
-
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.08
1-amino-2-(4-hydroxyphenyl)-ethyl phosphinic acid
-
pH 9.0
3.2 - 9.9
3,4-dihydroxyphenyl-L-alanine
0.1 - 1.3
3-fluoro-L-tyrosine
6.1
Ala
-
beta-elimination
0.8 - 12
beta-chloro-L-alanine
6.28
L-Dopa
recombinant enzyme, pH 8.5, 20ưC
5.3
L-Glu
-
mutant enzyme R100T/V283R
0.94
L-m-Tyr
-
transamination
0.8 - 2.9
O-benzoyl-L-Ser
4.15
Phe
-
beta-elimination
15.62
pyrocatechol
recombinant enzyme, L-DOPA formation is measured from dicatechol, pyruvate, and ammonium, pH 8.5, 20ưC
0.21 - 2.2
S-(2-nitrophenyl)-L-cysteine
0.1 - 1.86
S-(o-nitrophenyl)-L-Cys
0.27 - 0.36
S-(o-nitrophenyl)-L-cysteine
0.12 - 4.1
S-Benzyl-L-cysteine
1.57 - 5.4
S-ethyl-L-cysteine
0.52 - 56.4
S-methyl-L-Cys
2.32 - 10.5
S-methyl-L-cysteine
0.24
Tyr
-
beta-elimination
additional information
additional information
-
3.2
3,4-dihydroxyphenyl-L-alanine

-
wild type enzyme, in 0.1 M potassium phosphate buffer (pH 8.0) at 30ưC
4.6
3,4-dihydroxyphenyl-L-alanine
-
mutant enzyme T15A, at 30ưC
9.9
3,4-dihydroxyphenyl-L-alanine
Symbiobacterium sp.
-
pH 7.2, 60ưC
1.7
3-chloro-L-Ala

-
wild-type enzyme
2
3-chloro-L-Ala
-
mutant enzyme Y71F
2
3-chloro-L-Ala
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
3.2
3-chloro-L-Ala
-
mutant enzyme Y71F
11.4
3-chloro-L-Ala
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
13.3
3-chloro-L-Ala
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
46
3-chloro-L-Ala
-
mutant enzyme R100T/V283R
0.1
3-fluoro-L-Tyr

-
wild-type enzyme
0.1
3-fluoro-L-Tyr
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.2
3-fluoro-L-Tyr
-
mutant enzyme H343A
0.1
3-fluoro-L-tyrosine

recombinant wild-type enzyme, pH 8.0, 30ưC
1.3
3-fluoro-L-tyrosine
recombinant mutant S51A, pH 8.0, 30ưC
0.8
beta-chloro-L-alanine

recombinant mutant S51A, pH 8.0, 30ưC
2.12
beta-chloro-L-alanine
recombinant wild-type enzyme, pH 8.0, 30ưC
12
beta-chloro-L-alanine
Symbiobacterium sp.
-
pH 7.2, 60ưC
32
D-Ala

-
wild-type enzyme, alpha,beta-elimination
11
L-Ala

-
wild-type enzyme
103
L-Ala
-
mutant enzyme H343A
0.21
L-Asp

-
mutant enzyme R100T/V283R
54
L-Asp
-
mutant enzyme R100T/V283R
0.196
L-Phe

-
-
11
L-Ser

-
mutant enzyme H343A
18
L-Ser
-
wild-type enzyme
0.054
L-Tyr

-
-
0.2
L-Tyr
-
wild-type enzyme
0.2
L-Tyr
-
wild-type enzyme
0.2
L-Tyr
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.23
L-Tyr
Aeromonas phenologenes
-
-
0.32
L-Tyr
-
mutant enzyme R100T/V283R
2.2
L-Tyr
-
mutant enzyme H343A
0.18
L-tyrosine

recombinant enzyme, pH 8.5, 20ưC
0.19
L-tyrosine
Symbiobacterium sp.
-
pH 7.2, 60ưC
0.2
L-tyrosine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.22
L-tyrosine
-
mutant enzyme T15A, in 0.1 M potassium phosphate buffer (pH 8.0) at 30ưC
0.24
L-tyrosine
-
wild type enzyme, in 0.1 M potassium phosphate buffer (pH 8.0) at 30ưC
0.446
L-tyrosine
-
pH 8.5, 30ưC
1.3
L-tyrosine
recombinant mutant S51A, pH 8.0, 30ưC
0.8
O-benzoyl-L-Ser

-
mutant enzyme Y71F
2.9
O-benzoyl-L-Ser
-
mutant enzyme Y71F
3.6
phenol

recombinant enzyme, L-tyrosine formation is measured from phenol, pyruvate, and ammonium, pH 8.5, 20ưC
5.1
pyruvate

-
-
0.21
S-(2-nitrophenyl)-L-cysteine

recombinant wild-type enzyme, pH 8.0, 30ưC
2.2
S-(2-nitrophenyl)-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
0.1
S-(o-nitrophenyl)-L-Cys

-
wild-type enzyme
0.1
S-(o-nitrophenyl)-L-Cys
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.16
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme Y71F
0.5
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme H343A
1.32
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.86
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.27
S-(o-nitrophenyl)-L-cysteine

-
pH 8.0, 25ưC
0.36
S-(o-nitrophenyl)-L-cysteine
Symbiobacterium sp.
-
pH 7.2, 60ưC
0.05
S-benzyl-L-Cys

-
mutant enzyme Y71F
0.2
S-benzyl-L-Cys
-
wild-type enzyme
0.2
S-benzyl-L-Cys
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.2
S-benzyl-L-Cys
-
mutant enzyme H343A
5.1
S-benzyl-L-Cys
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
7
S-benzyl-L-Cys
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.12
S-Benzyl-L-cysteine

recombinant wild-type enzyme, pH 8.0, 30ưC
4.1
S-Benzyl-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
0.3
S-ethyl-L-Cys

-
mutant enzyme Y71F
1.2
S-ethyl-L-Cys
-
mutant enzyme H343A
6.6
S-ethyl-L-Cys
-
wild-type enzyme
1.57
S-ethyl-L-cysteine

recombinant wild-type enzyme, pH 8.0, 30ưC
5.4
S-ethyl-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
0.52
S-methyl-L-Cys

-
mutant enzyme Y71F
3.4
S-methyl-L-Cys
-
wild-type enzyme
3.4
S-methyl-L-Cys
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
12.99
S-methyl-L-Cys
-
mutant enzyme H343A
13.4
S-methyl-L-Cys
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
50
S-methyl-L-Cys
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
56.4
S-methyl-L-Cys
-
wild-type enzyme
2.32
S-methyl-L-cysteine

recombinant wild-type enzyme, pH 8.0, 30ưC
3.2
S-methyl-L-cysteine
-
pH 8.0, 25ưC
5.5
S-methyl-L-cysteine
Symbiobacterium sp.
-
pH 7.2, 60ưC
10.5
S-methyl-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
34
Ser

-
beta-elimination
additional information
additional information

-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
pH-independent kinetic constants for substrates bearing small substituents
-
additional information
additional information
steady-state kinetics
-
additional information
additional information
pre-steady state kinetics and steady state kinetic study, overview. TPL from Citrobacter freundii exhibits two basic pKas, with an average value of 7.8, in the pH dependence of kcat/Km, and kcat is pH-independent. The TPL reaction shows primary isotope effects of about 3 on kcat and 2 on kcat/Km for both enzymes, also suggesting that steps involving transfer of the alpha-proton are partially rate-limiting. The primary isotope effect increases to 5.4 and 3.8, respectively, for kcat and kcat/Km with alpha-[2H]-3-fluoro-L-tyrosine with H343A TPL, compared to 3.9 and 2.2 for wild-type TPL. Stopped-flow measurements, the reaction of TPL with L-tyrosine and 3-fluoro-L-tyrosine in the stopped-flow spectrophotometer also shows rapid formation of quinonoid intermediate
-
additional information
additional information
pre-steady-state kinetic and steady-state kinetics, kinetic analysis of recombinant wild-type and mutant enzymes, stopped-flow kinetics of enzyme mutant F448A
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.31 - 0.68
3,4-dihydroxyphenyl-L-alanine
0.0065 - 3
3-chloro-L-Ala
0.18 - 1.4
3-fluoro-L-Tyr
0.012 - 1.4
3-fluoro-L-tyrosine
0.00153
Ala
-
beta-elimination
0.15 - 6
beta-chloro-L-alanine
0.03
L-Ala
-
wild-type enzyme
0.54
L-Dopa
recombinant enzyme, pH 8.5, 20ưC
0.00268
L-m-Tyr
-
transamination
0.000034 - 3.5
L-tyrosine
0.15 - 8.3
O-benzoyl-L-Ser
3.9
O-benzyl-L-Cys
wild-type enzyme
0.0008
Phe
-
beta-elimination
0.44
phenol
recombinant enzyme, L-tyrosine formation is measured from phenol, pyruvate, and ammonium, pH 8.5, 20ưC
2.68
pyrocatechol
recombinant enzyme, L-DOPA formation is measured from dicatechol, pyruvate, and ammonium, pH 8.5, 20ưC
0.26 - 9.7
S-(2-nitrophenyl)-L-cysteine
0.024 - 9.7
S-(o-nitrophenyl)-L-Cys
0.0091 - 7.4
S-(o-nitrophenyl)-L-cysteine
0.02
S-benzoyl-L-Cys
-
mutant enzyme H343A
0.0012 - 0.52
S-benzyl-L-Cys
0.015 - 0.63
S-Benzyl-L-cysteine
0.000217 - 3.88
S-ethyl-L-Cys
0.083 - 5.4
S-ethyl-L-cysteine
0.000383 - 0.9
S-methyl-L-Cys
0.044 - 1.13
S-methyl-L-cysteine
0.2
Tyr
-
beta-elimination
additional information
additional information
-
0.31
3,4-dihydroxyphenyl-L-alanine

-
wild type enzyme, in 0.1 M potassium phosphate buffer (pH 8.0) at 30ưC
0.68
3,4-dihydroxyphenyl-L-alanine
-
mutant enzyme T15A, at 30ưC
0.0065
3-chloro-L-Ala

-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.02
3-chloro-L-Ala
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.053
3-chloro-L-Ala
-
mutant enzyme Y71F
0.7
3-chloro-L-Ala
-
wild-type enzyme
0.7
3-chloro-L-Ala
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.13
3-chloro-L-Ala
-
mutant enzyme R100T/V283R
3
3-chloro-L-Ala
-
wild-type enzyme
3
3-chloro-L-Ala
wild-type enzyme
0.18
3-fluoro-L-Tyr

-
mutant enzyme H343A
1.4
3-fluoro-L-Tyr
-
wild-type enzyme
1.4
3-fluoro-L-Tyr
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.012
3-fluoro-L-tyrosine

recombinant mutant S51A, pH 8.0, 30ưC
1.4
3-fluoro-L-tyrosine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.15
beta-chloro-L-alanine

recombinant mutant S51A, pH 8.0, 30ưC
6
beta-chloro-L-alanine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.004
D-Ala

-
mutant enzyme H343A
0.008
D-Ala
-
racemization
0.01
D-Ala
-
wild-type enzyme
0.748
L-Phe

-
-
0.07
L-Ser

-
mutant enzyme H343A
0.17
L-Ser
-
wild-type enzyme
0.11
L-Tyr

-
mutant enzyme R100T/V283R
0.42
L-Tyr
-
mutant enzyme H343A
2.2
L-Tyr
wild-type enzyme
3.5
L-Tyr
-
wild-type enzyme
3.5
L-Tyr
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.000034
L-tyrosine

pH 8.0, 25ưC, recombinant mutant F448H
0.00013
L-tyrosine
pH 8.0, 25ưC, mutant enzyme K256H
0.00016
L-tyrosine
pH 8.0, 25ưC, recombinant mutant F449A
0.00026
L-tyrosine
pH 8.0, 25ưC, recombinant mutant F448A
0.001
L-tyrosine
pH 8.0, 25ưC, mutant enzyme K256A
0.005
L-tyrosine
pH 8.0, 25ưC, recombinant mutant F448L
0.03
L-tyrosine
recombinant mutant S51A, pH 8.0, 30ưC
0.12
L-tyrosine
pH 8.0, 25ưC, mutant enzyme K256R
1.2
L-tyrosine
-
mutant enzyme T15A, in 0.1 M potassium phosphate buffer (pH 8.0) at 30ưC
1.72
L-tyrosine
recombinant enzyme, pH 8.5, 20ưC
1.8
L-tyrosine
-
wild type enzyme, in 0.1 M potassium phosphate buffer (pH 8.0) at 30ưC
3.5
L-tyrosine
pH 8.0, 25ưC, recombinant wild-type enzyme
3.5
L-tyrosine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.15
O-benzoyl-L-Ser

-
mutant enzyme Y71F
8.3
O-benzoyl-L-Ser
-
wild-type enzyme
8.3
O-benzoyl-L-Ser
wild-type enzyme
0.26
S-(2-nitrophenyl)-L-cysteine

pH 8.0, 25ưC, recombinant mutant F448L
0.36
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant mutant F449A
0.6
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448H
2.5
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448A
8.7
S-(2-nitrophenyl)-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
9.65
S-(2-nitrophenyl)-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
9.7
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant wild-type enzyme
0.024
S-(o-nitrophenyl)-L-Cys

-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.041
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.25
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme Y71F
1.55
S-(o-nitrophenyl)-L-Cys
-
mutant enzyme H343A
5.1
S-(o-nitrophenyl)-L-Cys
-
wild-type enzyme
5.1
S-(o-nitrophenyl)-L-Cys
wild-type enzyme
5.1
S-(o-nitrophenyl)-L-Cys
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
9.7
S-(o-nitrophenyl)-L-Cys
wild-type enzyme
0.0091
S-(o-nitrophenyl)-L-cysteine

pH 8.0, 25ưC, mutant enzyme K256A
0.027
S-(o-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, mutant enzyme K256H
0.17
S-(o-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, mutant enzyme K256R
7.4
S-(o-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, mutant enzyme E69D/K256R
0.0012
S-benzyl-L-Cys

-
mutant enzyme Y71F
0.0067
S-benzyl-L-Cys
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.02
S-benzyl-L-Cys
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.5
S-benzyl-L-Cys
wild-type enzyme
0.5
S-benzyl-L-Cys
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.52
S-benzyl-L-Cys
-
wild-type enzyme
0.015
S-Benzyl-L-cysteine

recombinant mutant S51A, pH 8.0, 30ưC
0.63
S-Benzyl-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.000217
S-ethyl-L-Cys

-
mutant enzyme Y71F
0.0025
S-ethyl-L-Cys
pH 8.0, 25ưC, mutant enzyme K256A
0.0088
S-ethyl-L-Cys
pH 8.0, 25ưC, mutant enzyme K256H
0.02
S-ethyl-L-Cys
pH 8.0, 25ưC, mutant enzyme K256R
0.37
S-ethyl-L-Cys
pH 8.0, 25ưC, mutant enzyme E69D/K256R
1.4
S-ethyl-L-Cys
-
mutant enzyme H343A
1.7
S-ethyl-L-Cys
wild-type enzyme
3.88
S-ethyl-L-Cys
-
wild-type enzyme
0.083
S-ethyl-L-cysteine

recombinant mutant S51A, pH 8.0, 30ưC
0.12
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F449A
0.23
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448L
0.27
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448H
0.51
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448A
3.9
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant wild-type enzyme
5.4
S-ethyl-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.000383
S-methyl-L-Cys

-
mutant enzyme Y71F
0.0016
S-methyl-L-Cys
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.0052
S-methyl-L-Cys
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.15
S-methyl-L-Cys
-
mutant enzyme H343A
0.9
S-methyl-L-Cys
-
wild-type enzyme
0.9
S-methyl-L-Cys
wild-type enzyme
0.9
S-methyl-L-Cys
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.044
S-methyl-L-cysteine

recombinant mutant S51A, pH 8.0, 30ưC
1.13
S-methyl-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.001
Ser

-
transamination
0.012
Ser
-
beta-elimination
additional information
additional information

-
pH-independent kinetic constants for substrates bearing small substituents
-
additional information
additional information
the turnover-numbers of the mutant enzyme R381I, R381V and R381A for the substrates S-(o-nitrophenyl)-L-Cys, 3-chloro-L-Ala, O-benzoyl-L-Ser and S-methyl-L-Cys are comparable to those of the wild-type enzyme
-
additional information
additional information
-
the turnover-numbers of the mutant enzyme R381I, R381V and R381A for the substrates S-(o-nitrophenyl)-L-Cys, 3-chloro-L-Ala, O-benzoyl-L-Ser and S-methyl-L-Cys are comparable to those of the wild-type enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0092 - 14
3-fluoro-L-tyrosine
0.19 - 2.84
beta-chloro-L-alanine
0.09
L-Dopa
recombinant enzyme, pH 8.5, 20ưC
0.0000008 - 17.5
L-tyrosine
0.12
phenol
recombinant enzyme, L-tyrosine formation is measured from phenol, pyruvate, and ammonium, pH 8.5, 20ưC
0.0062 - 46
S-(2-nitrophenyl)-L-cysteine
0.0037 - 5.26
S-Benzyl-L-cysteine
0.000088 - 3.43
S-ethyl-L-cysteine
0.0042 - 0.49
S-methyl-L-cysteine
0.0092
3-fluoro-L-tyrosine

recombinant mutant S51A, pH 8.0, 30ưC
14
3-fluoro-L-tyrosine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.19
beta-chloro-L-alanine

recombinant mutant S51A, pH 8.0, 30ưC
2.84
beta-chloro-L-alanine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.0000008
L-tyrosine

pH 8.0, 25ưC, recombinant mutant F449A
0.00037
L-tyrosine
pH 8.0, 25ưC, recombinant mutant F448H
0.0013
L-tyrosine
pH 8.0, 25ưC, recombinant mutant F448A
0.022
L-tyrosine
recombinant mutant S51A, pH 8.0, 30ưC
0.035
L-tyrosine
pH 8.0, 25ưC, recombinant mutant F448L
9.58
L-tyrosine
recombinant enzyme, pH 8.5, 20ưC
17.5
L-tyrosine
pH 8.0, 25ưC, recombinant wild-type enzyme
17.5
L-tyrosine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.0062
S-(2-nitrophenyl)-L-cysteine

pH 8.0, 25ưC, recombinant mutant F449A
3.3
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448H
3.9
S-(2-nitrophenyl)-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
4.5
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448L
9.6
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448A
46
S-(2-nitrophenyl)-L-cysteine
pH 8.0, 25ưC, recombinant wild-type enzyme
46
S-(2-nitrophenyl)-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.0037
S-Benzyl-L-cysteine

recombinant mutant S51A, pH 8.0, 30ưC
5.26
S-Benzyl-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.000088
S-ethyl-L-cysteine

pH 8.0, 25ưC, recombinant mutant F449A
0.015
S-ethyl-L-cysteine
recombinant mutant S51A, pH 8.0, 30ưC
0.17
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448H
0.59
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant wild-type enzyme
0.965
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448A
0.994
S-ethyl-L-cysteine
pH 8.0, 25ưC, recombinant mutant F448L
3.43
S-ethyl-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
0.0042
S-methyl-L-cysteine

recombinant mutant S51A, pH 8.0, 30ưC
0.49
S-methyl-L-cysteine
recombinant wild-type enzyme, pH 8.0, 30ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.44
1-amino-2-(4-hydroxyphenyl)-ethyl phosphinic acid
-
-
100
1-amino-2-(4-hydroxyphenyl)-ethyl phosphonic acid
-
-
0.135 - 3.4
2-aza-L-tyrosine
1.5
L-bishomotyrosine
pH 8.0, 30ưC, recombinant enzyme
0.8
L-homotyrosine
pH 8.0, 30ưC, recombinant enzyme
7
L-tryptophan
pH 8.0, 30ưC
0.2
L-tyrosine
pH 8.0, 30ưC
1.04
O-methyl-L-bishomotyrosine
pH 8.0, 30ưC, recombinant enzyme
0.8
O-methyl-L-homotyrosine
pH 8.0, 30ưC, recombinant enzyme
additional information
additional information
-
0.135
2-aza-L-tyrosine

-
25ưC, pH 7.8
3.4
2-aza-L-tyrosine
-
25ưC, pH 7.8
3
L-Asp

-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
3.5
L-Asp
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using L-Tyr as a substrate
3.5
L-Asp
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using S-(o-nitrophenyl)-L-Cys as a substrate
3.6
L-Asp
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
5
L-Glu

-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using L-Tyr as a substrate
5
L-Glu
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using S-(o-nitrophenyl)-L-Cys as a substrate
66
L-Glu
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
100
L-Glu
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.33
L-homoserine

-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using L-Tyr as a substrate
1.33
L-homoserine
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using S-(o-nitrophenyl)-L-Cys as a substrate
69
L-homoserine
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
82
L-homoserine
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
0.73
L-Met

-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using L-Tyr as a substrate
0.73
L-Met
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using S-(o-nitrophenyl)-L-Cys as a substrate
4.2
L-Met
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
11
L-Met
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.7
L-Phe

-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using L-Tyr as a substrate
1.7
L-Phe
-
wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25ưC, using S-(o-nitrophenyl)-L-Cys as a substrate
17
L-Phe
-
mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25ưC
22
L-Phe
-
mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25ưC
1.7
L-phenylalanine

-
-
2
L-phenylalanine
pH 8.0, 30ưC
additional information
additional information

rapid scanning stopped-flow kinetic experiments, kinetics
-
additional information
additional information
rapid-scanning stopped-flow experiments, steady-state and pre-steady-state inhibition kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.