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The expected taxonomic range for this enzyme is: Vicia sativa
Synonyms
(R)-Oxynitrilase,
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(R)-mandelonitrile = cyanide + benzaldehyde
(R)-mandelonitrile = cyanide + benzaldehyde
no activity with aliphatic substrates
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(R)-mandelonitrile = cyanide + benzaldehyde
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(R)-mandelonitrile benzaldehyde lyase (cyanide and aromatic aldehyde-forming)
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cyanide + 1,3-benzodioxole-5-carbaldehyde
(2S)-1,3-benzodioxol-5-yl(hydroxy)ethanenitrile
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93% yield, 88% enantiomeric excess
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cyanide + 4-fluorobenzaldehyde
(2R)-(4-fluorophenyl)(hydroxy)ethanenitrile
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97% yield, 91% enantiomeric excess
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cyanide + 4-methoxybenzaldehyde
(2R)-hydroxy(4-methoxyphenyl)ethanenitrile
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52% yield, 94% enantiomeric excess
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cyanide + benzaldehyde
(R)-mandelonitrile
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100% yield, 99% enantiomeric excess
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cyanide + furan-2-carbaldehyde
(2S)-furan-2-yl(hydroxy)ethanenitrile
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96% yield, 97% enantiomeric excess. (S)-configuration is due to the Cahn-Ingold-Prelog rules
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cyanide + pyridine-3-carbaldehyde
(2R)-hydroxy(pyridin-3-yl)ethanenitrile
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71% yield, 3% enantiomeric excess
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additional information
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no activity with heptanal
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brenda
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defatted meal
brenda
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Han, S.Q.; Ouyang, P.K.; Wei, P.; Hu, Y.
Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L
Biotechnol. Lett.
28
1909-1912
2006
Vicia sativa
brenda
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4.1.2.B6 aromatic (R)-hydroxynitrile lyase
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