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The expected taxonomic range for this enzyme is: Vanilla planifolia
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VAN

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ferulate + H2O = vanillin + acetate
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ferulate acetate lyase (vanillin-forming)
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ferulate + H2O
vanillin + acetate
ferulic acid 4-O-beta-D-glucopyranoside
vanillin 4-O-beta-D-glucopyranoside + acetyl-CoA
additional information
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no activity with p-coumaric acid, caffeic acid, p-coumaric acid glucoside, caffeic acid glucoside, feruloyl-CoA, p-coumaroyl-CoA. and caffeoyl-CoA
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ferulate + H2O

vanillin + acetate
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ferulate + H2O
vanillin + acetate
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ferulic acid 4-O-beta-D-glucopyranoside

vanillin 4-O-beta-D-glucopyranoside + acetyl-CoA
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ferulic acid 4-O-beta-D-glucopyranoside
vanillin 4-O-beta-D-glucopyranoside + acetyl-CoA
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additional information
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ATP and NAD+ are not required as cofactors
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brenda
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UniProt
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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A0A0F7G352_VANPL
356
0
39031
TrEMBL
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100000
gel filtration, the enzyme exists as 50000, 75000 and 100000 Da protein
50000
gel filtration, the enzyme exists as 50000, 75000 and 100000 Da protein
75000
gel filtration, the enzyme exists as 50000, 75000 and 100000 Da protein
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x * 36000, SDS-PAGE
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x * 23890, mature form, calculated from amino acid sequence
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x * 25000, mature form, SDS-PAGE
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x * 39150, calculated from amino acid sequence
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x * 39150, immature unprocessed form, calculated from amino acid sequence
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x * 40000, immature unprocessed form, SDS-PAGE
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expressed in Nicotiana benthamiana leaves
expressed in Saccharomyces cerevisiae strain Y06460 and Nicotiana benthamiana
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Gallage, N.J.; Hansen, E.H.; Kannangara, R.; Olsen, C.E.; Motawia, M.S.; Jorgensen, K.; Holme, I.; Hebelstrup, K.; Grisoni, M.; Moller, B.L.
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme
Nat. Commun.
5
4037
2014
Vanilla planifolia
brenda
Gallage, N.J.; J?rgensen, K.; Janfelt, C.; Nielsen, A.J.Z.; Naake, T.; Dunski, E.; Dalsten, L.; Grisoni, M.; M?ller, B.L.
The intracellular localization of the vanillin biosynthetic machinery in pods of Vanilla planifolia
Plant Cell Physiol.
59
304-318
2018
Vanilla planifolia (A0A0F7G352)
brenda
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