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IUBMB Comments The enzyme has been purified from vanilla pods of the orchid Vanilla planifolia . It is highly specific for 4-coumarate. Similar compounds such as cinnamate, caffeate, sinapate and o -coumarate are not substrates.
Synonyms
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4-hydroxybenzaldehyde synthase
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4HBS
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3-hydroxy-3-(4-hydroxyphenyl)propanoate = 4-hydroxybenzaldehyde + acetate
(1b)
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4-coumarate + H2O = 3-hydroxy-3-(4-hydroxyphenyl)propanoate
(1a)
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4-coumarate + H2O = 4-hydroxybenzaldehyde + acetate
overall reaction
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4-coumarate acetate-lyase (4-hydroxybenzaldehyde-forming)
The enzyme has been purified from vanilla pods of the orchid Vanilla planifolia. It is highly specific for 4-coumarate. Similar compounds such as cinnamate, caffeate, sinapate and o-coumarate are not substrates.
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4-coumarate + H2O
4-hydroxybenzaldehyde + acetate
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Substrates: -
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additional information
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Substrates: no activity with ferulic acid, sinapic acid and cinnamic acid
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4-coumarate + H2O
4-hydroxybenzaldehyde + acetate
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Substrates: -
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additional information
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the enzyme does not require any cofactor
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ascorbate
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the reaction with 4-coumarate and dithiothreitol is almost completely inhibited in the presence of 1 mM ascorbate
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coenzyme A
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101% activity in the presence of 10 mM compared to dithiothreitol
dithioerythritol
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101% activity in the presence of 10 mM compared to dithiothreitol
dithiothreitol
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100% activity in the presence of 10 mM
additional information
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2-mercaptoethanol, glutathione (reduced or oxidized) or cysteine are less effective thiol reagents
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Highest Expressing Human Cell Lines
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Gene Links
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4HBS_VANPL
352
0
38616
Swiss-Prot
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ammonium sulfate precipitation, Mono Q column chromatography, and Superdex HR 200 gel filtration
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Podstolski, A.; Havkin-Frenkel, D.; Malinowski, J.; Blount, J.W.; Kourteva, G.; Dixon, R.A.
Unusual 4-hydroxybenzaldehyde synthase activity from tissue cultures of the vanilla orchid Vanilla planifolia
Phytochemistry
61
611-620
2002
Vanilla planifolia
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