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EC Tree
IUBMB Comments The enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway. In the direction of aldol synthesis the enzyme catalyses the formation of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate at a similar ratio. It shows no activity with 2-dehydro-3-deoxy-D-gluconate 6-phosphate. cf. EC 4.1.2.14, 2-dehydro-3-deoxy-phosphogluconate aldolase.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
KDG-specific aldolase, KDGA,
Pto1279 ,
more
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2-dehydro-3-deoxy-D-gluconate = pyruvate + D-glyceraldehyde
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2-dehydro-3-deoxy-D-gluconate D-glyceraldehyde-3-phosphate-lyase (pyruvate-forming)
The enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway. In the direction of aldol synthesis the enzyme catalyses the formation of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate at a similar ratio. It shows no activity with 2-dehydro-3-deoxy-D-gluconate 6-phosphate. cf. EC 4.1.2.14, 2-dehydro-3-deoxy-phosphogluconate aldolase.
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2-dehydro-3-deoxy-D-galactonate
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
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?
2-dehydro-3-deoxy-D-galactonate
pyruvate + D-glyceraldehyde
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-
?
2-dehydro-3-deoxy-D-galactonate
pyruvate + D-glyceraldehyde
the enzyme shows substrate promiscuity, as measured in the direction of aldol synthesis. The enzyme catalyzes the formation of both 2-dehydro-3-deoxy-D-gluconate and its C-4 epimer 2-keto-3-deoxygalactonate from D- or L-glyceraldehyde and pyruvate at a similar ratio, indicating lack of stereospecific control in formation and cleavage of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate
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r
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
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?
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2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
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-
?
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0.3 - 0.4
2-dehydro-3-deoxy-D-gluconate
2.5 - 8
2-dehydro-3-deoxy-D-gluconate 6-phosphate
4.6
D-glyceraldehyde
pH 6.2, 60°C
2.7
pyruvate
pH 6.2, 60°C
0.3
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
0.4
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
2.5
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
8
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
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24.2 - 26.7
2-dehydro-3-deoxy-D-gluconate
0.34 - 0.36
2-dehydro-3-deoxy-D-gluconate 6-phosphate
24.2
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
26.7
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
0.34
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
0.36
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
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52 - 89
2-dehydro-3-deoxy-D-gluconate
0.04 - 0.14
2-dehydro-3-deoxy-D-gluconate 6-phosphate
52
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
89
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
0.04
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
0.14
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
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48
pH 6.2, 60°C, substrate: 2-dehydro-3-deoxy-D-gluconate
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4.5 - 7.5
pH 4.5: 50% of maximal activity, pH 7.5: about 50% of maximal activity
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UniProt
brenda
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physiological function
the enzyme is involved in the nonphosphorylative variant of the EntnerĀDoudoroff pathway
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YAGE_ECOLI
Escherichia coli (strain K12)
302
0
32530
Swiss-Prot
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KDGA_PICTO
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
266
0
30391
Swiss-Prot
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KDGA_THETE
306
0
33287
Swiss-Prot
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A0A7L6M8B4_9ENTR
302
0
32830
TrEMBL
-
A0A7X9QNC7_9ENTR
302
0
32713
TrEMBL
-
A0A645F1N1_9ZZZZ
174
0
19640
TrEMBL
other Location (Reliability: 2 )
A0A7Z3LKF5_ENTAS
302
0
32787
TrEMBL
-
A0A7T5XQ07_9ENTR
302
0
32662
TrEMBL
-
A0A7C8G9E0_9ENTR
302
0
32840
TrEMBL
-
A0A4R0G7L3_9ENTR
302
0
32695
TrEMBL
-
A0A7G5GFL3_9ENTR
302
0
32703
TrEMBL
-
A0A517DNZ8_9FIRM
297
0
32336
TrEMBL
-
A0A7V7GJ25_9ENTR
302
0
32762
TrEMBL
-
A0A151AFC9_9EURY
295
0
30885
TrEMBL
-
A0A564VGS3_BIFLI
318
0
34022
TrEMBL
-
A0A6G3F8Q9_9ENTR
302
0
32646
TrEMBL
-
A0A7Z8FNU2_9ENTR
302
0
32683
TrEMBL
-
A0A376PKQ1_ECOLX
302
0
32530
TrEMBL
-
A0A4R0FQH4_9ENTR
302
0
32717
TrEMBL
-
A0A6L3XB88_9ENTR
166
0
18174
TrEMBL
-
A0A6J5AKX8_9BURK
319
0
34565
TrEMBL
-
A0A7M1R507_9ENTR
302
0
32655
TrEMBL
-
A0A7X9NC85_ENTAS
302
0
32707
TrEMBL
-
A0A7D6Y536_9ENTR
302
0
32708
TrEMBL
-
A0A7H9JPQ3_ENTCL
302
0
32708
TrEMBL
-
A0A7Y8YNJ1_9ENTR
302
0
32656
TrEMBL
-
A0A7W3G5L4_9ENTR
302
0
32703
TrEMBL
-
A0A6N8Q0S0_ECOLX
302
0
32766
TrEMBL
-
A0A7H0N6A2_9ENTR
302
0
32632
TrEMBL
-
A0A645FR21_9ZZZZ
253
0
26762
TrEMBL
other Location (Reliability: 3 )
A0A7V7G627_9ENTR
302
0
32631
TrEMBL
-
A0A6G8NVP7_9BURK
309
0
33473
TrEMBL
-
A0A7D6X514_9ENTR
302
0
32662
TrEMBL
-
A0A6S7E0D8_9BURK
302
0
32248
TrEMBL
-
A0A7D6UFW7_9ENTR
302
0
32593
TrEMBL
-
A0A7U0KCD4_9ENTR
302
0
32688
TrEMBL
-
A0A7H0MTF9_9ENTR
302
0
32622
TrEMBL
-
A0A1D8IHG3_9BURK
302
0
32190
TrEMBL
-
A0A7W2VWI9_9ENTR
302
0
32785
TrEMBL
-
A0A6S7CXK8_9BURK
302
0
32031
TrEMBL
-
A0A6S6YYQ1_9BURK
302
0
32217
TrEMBL
-
A0A7L6J6J6_9ENTR
302
0
32830
TrEMBL
-
A0A644XLZ0_9ZZZZ
297
0
32854
TrEMBL
other Location (Reliability: 3 )
A0A564S0M9_BIFLI
322
0
34254
TrEMBL
-
A0A7Y8N9X7_9ENTR
302
0
32751
TrEMBL
-
A0A7V9A6D2_9BACT
313
0
34577
TrEMBL
-
A0A7H0U8P2_9ENTR
302
0
32622
TrEMBL
-
A0A4P6XZ31_9ENTR
302
0
32610
TrEMBL
-
A0A6C2DJT1_9ENTR
302
0
32808
TrEMBL
-
A0A7L5WQ13_9ENTR
302
0
32869
TrEMBL
-
A0A644UNT5_9ZZZZ
292
0
32042
TrEMBL
other Location (Reliability: 2 )
A0A331WC35_9ENTR
302
0
32734
TrEMBL
-
A0A6S6Z737_9BURK
317
0
34014
TrEMBL
-
A0A6I1YRX5_9ACTN
308
0
32188
TrEMBL
-
A0A7Z2FMF2_ENTAS
302
0
32586
TrEMBL
-
A0A7Z3N2W7_ENTAS
302
0
32645
TrEMBL
-
A0A6G4MQP5_9ENTR
302
0
32705
TrEMBL
-
A0A6I4KNZ2_9ENTR
244
0
26114
TrEMBL
-
A0A5A9X048_9ENTR
302
0
32755
TrEMBL
-
A0A7W2VBF4_9ENTR
302
0
32656
TrEMBL
-
A0A7V9A7B6_9BACT
307
0
33752
TrEMBL
-
A0A6J4ZJN2_9BURK
302
0
32074
TrEMBL
-
A0A4Y8IM15_9ENTR
302
0
32840
TrEMBL
-
A0A0J0GLJ0_ENTAS
302
0
32648
TrEMBL
-
A0A6L4XH85_ECOLX
302
0
32676
TrEMBL
-
A0A7H8U9Y4_ENTCL
302
0
32583
TrEMBL
-
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32000
4 * 32000, SDS-PAGE
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homotetramer
4 * 32000, SDS-PAGE
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expression in Escherichia coli
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Reher, M.; Fuhrer, T.; Bott, M.; Schoenheit, P.
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase
J. Bacteriol.
192
964-974
2010
Picrophilus torridus (Q6KZI8), Picrophilus torridus
brenda
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