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Information on EC 4.1.2.28 - 2-dehydro-3-deoxy-D-pentonate aldolase
for references in articles please use BRENDA:EC4.1.2.28
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EC Tree 4 Lyases
4.1 Carbon-carbon lyases
4.1.2 Aldehyde-lyases
4.1.2.28 2-dehydro-3-deoxy-D-pentonate aldolase
The enzyme appears in viruses and cellular organisms
showSynonyms
2-dehydro-3-deoxy-d-pentonate aldolase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-keto-3-deoxy-D-pentonate aldolase
-
-
-
-
3-Deoxy-D-pentulosonic acid aldolase
-
-
-
-
aldolase, 2-keto-3-deoxy-D-pentonate
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-Dehydro-3-deoxy-D-pentonate = pyruvate + glycolaldehyde
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
condensation
-
-
-
-
elimination
-
-
of an aldehyde, C-C bond cleavage
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
D-xylose degradation IV, D-xylose degradation to ethylene glycol (engineered)
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-D-pentonate glycolaldehyde-lyase (pyruvate-forming)
-
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CAS REGISTRY NUMBER
COMMENTARY
55326-36-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-D-pentonate
pyruvate + glycolaldehyde
-
Substrates: r, specific for D-isomer
Products: -
Products: -
?
2-dehydro-3-deoxy-D-pentonate
?
-
Substrates: enzyme is involved in a new pathway of D-xylose metabolism
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-D-pentonate
?
-
Substrates: enzyme is involved in a new pathway of D-xylose metabolism
Products: -
Products: -
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). YJHH_ECOLI
Escherichia coli (strain K12)
301
0
32722
Swiss-Prot
other Location (Reliability: 3)
A0A0H3CI50_ENTCC
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56)
302
0
32702
TrEMBL
-
A0A144Y612_9ENTR
302
0
32755
TrEMBL
Secretory Pathway (Reliability: 2)
A0A2J0PYF5_9ENTR
302
0
32761
TrEMBL
Mitochondrion (Reliability: 4)
A0A3P8JSZ8_RAOTE
302
0
32456
TrEMBL
other Location (Reliability: 3)
A0A427KL77_ENTCL
302
0
32710
TrEMBL
other Location (Reliability: 1)
A0A822WQI4_9ENTR
302
0
32770
TrEMBL
other Location (Reliability: 4)
A0A837FJU4_9ENTR
302
0
32721
TrEMBL
-
A0A837LHW0_9ENTR
302
0
32591
TrEMBL
Secretory Pathway (Reliability: 2)
A0A9Q2WGY7_9ENTR
302
0
32703
TrEMBL
-
A0AA42PTM3_9ENTR
302
0
32637
TrEMBL
other Location (Reliability: 4)
A0AA96M581_9ENTR
302
0
32652
TrEMBL
Secretory Pathway (Reliability: 2)
A0AAE2EGV5_ENTCL
302
0
32665
TrEMBL
other Location (Reliability: 3)
A0AAE4E835_9ENTR
302
0
32720
TrEMBL
-
A0AAE8QU80_9ENTR
302
0
32717
TrEMBL
other Location (Reliability: 5)
A0AAI9D446_9ENTR
302
0
32678
TrEMBL
-
A0AAP8GNR0_9ENTR
302
0
32611
TrEMBL
Mitochondrion (Reliability: 3)
A0AAP9AJH9_9ENTR
302
0
32605
TrEMBL
other Location (Reliability: 1)
A0AAU7FV20_9ENTR
302
0
32634
TrEMBL
-
A0AAW3WPM4_SERFO
302
0
32769
TrEMBL
other Location (Reliability: 2)
A0AAX1WI31_9ENTR
302
0
32693
TrEMBL
Secretory Pathway (Reliability: 1)
A0AAX3LCI2_9ENTR
302
0
32840
TrEMBL
other Location (Reliability: 3)
A0ABD4K8G6_9ENTR
302
0
32674
TrEMBL
Secretory Pathway (Reliability: 1)
A0ABD7QEH6_RAOOR
302
0
32235
TrEMBL
other Location (Reliability: 4)
B7MMI3_ECO45
Escherichia coli O45:K1 (strain S88 / ExPEC)
301
0
32722
TrEMBL
-
B7NGU5_ECOLU
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
301
0
32722
TrEMBL
-
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
biosynthesis route for ethylene glycol production from D-xylose, consisting of conversion steps D-xylose to D-xylonate to 2-dehydro-3-deoxy-D-pentonate to glycoaldehyde to ethylene glycol. Implementation of the respective enzymes, D-xylose dehydrogenase, D-xylonate dehydratase, 2-dehydro-3-deoxy-D-pentonate aldolase, and glycoaldehyde reductase, in a metabolically engineered Escherichia coli, in which the D-xylose to D-xylulose reaction is prevented by disrupting the D-xylose isomerase gene, leads to a construct producing 11.7 g/l of ethylene glycol from 40.0 g/l of D-xylose
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dahms, A.S.
3 deoxy-D-Pentulosonic acid aldolase and its role in a new pathway of D-xylose degradation
Biochem. Biophys. Res. Commun.
60
1433-1439
1974
Pseudomonas sp.
brenda
Liu, H.; Ramos, K.R.; Valdehuesa, K.N.; Nisola, G.M.; Lee, W.K.; Chung, W.J.
Biosynthesis of ethylene glycol in Escherichia coli
Appl. Microbiol. Biotechnol.
97
3409-3417
2013
Escherichia coli
brenda
EXTERNAL LINKS (specific for EC-Number 4.1.2.28)
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