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EC Tree
IUBMB Comments The enzyme, which exists in all vertebrates, decarboxylates ethylmalonyl-CoA, a potentially toxic compound that is formed in low amounts by the activity of EC 6.4.1.2, acetyl-CoA carboxylase and EC 6.4.1.3, propanoyl-CoA carboxylase. It prefers the S isomer, and can decarboxylate (R)-ethylmalonyl-CoA with lower efficiency. cf. EC 7.2.4.1, (S)-methylmalonyl-CoA decarboxylase (sodium-transporting).
The taxonomic range for the selected organisms is: Mus musculus The enzyme appears in selected viruses and cellular organisms
Synonyms
echdc1, ethylmalonyl-coa decarboxylase,
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ethylmalonyl-CoA decarboxylase
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(S)-ethylmalonyl-CoA carboxy-lyase (butanoyl-CoA-forming)
The enzyme, which exists in all vertebrates, decarboxylates ethylmalonyl-CoA, a potentially toxic compound that is formed in low amounts by the activity of EC 6.4.1.2, acetyl-CoA carboxylase and EC 6.4.1.3, propanoyl-CoA carboxylase. It prefers the S isomer, and can decarboxylate (R)-ethylmalonyl-CoA with lower efficiency. cf. EC 7.2.4.1, (S)-methylmalonyl-CoA decarboxylase (sodium-transporting).
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(S)-ethylmalonyl-CoA
butanoyl-CoA + CO2
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best substrate, it is likely that the enzyme catalyzes preferentially the decarboxylation of one of the two isomers. Recombinant ethylmalonyl-CoA decarboxylase acts preferentially on (S)-ethylmalonyl-CoA
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methylmalonyl-CoA
propanoyl-CoA + CO2
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lower activity
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additional information
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the enzyme shows no malonyl-CoA decarboxylase or enoyl-CoA hydratase activity
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MgATP2-
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inhibits the enzyme slightly
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0.00096 - 0.0065
(S)-ethylmalonyl-CoA
0.0031 - 0.0151
methylmalonyl-CoA
0.00096
(S)-ethylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in absence of ATP-Mg2-
0.0065
(S)-ethylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in presence of 5 mM ATP-Mg2-
0.0031
methylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in absence of ATP-Mg2-
0.0151
methylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in presence of 5 mM ATP-Mg2-
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9.5 - 10
(S)-ethylmalonyl-CoA
1.61 - 1.81
methylmalonyl-CoA
9.5
(S)-ethylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in absence of ATP-Mg2-
10
(S)-ethylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in presence of 5 mM ATP-Mg2-
1.61
methylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in absence of ATP-Mg2-
1.81
methylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in presence of 5 mM ATP-Mg2-
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1500 - 9900
(S)-ethylmalonyl-CoA
110 - 510
methylmalonyl-CoA
1500
(S)-ethylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in presence of 5 mM ATP-Mg2-
9900
(S)-ethylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in absence of ATP-Mg2-
110
methylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in presence of 5 mM ATP-Mg2-
510
methylmalonyl-CoA
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pH 7.1, 30°C, recombinant enzyme in absence of ATP-Mg2-
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8800
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purified recombinant ECHDC1, pH 7.1, 30°C
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UniProt
brenda
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brenda
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brenda
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brenda
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brenda
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ECHD1_MOUSE
322
0
35467
Swiss-Prot
other Location (Reliability: 2 )
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34000
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x * 34000, recombinant ECHDC1, SDS-PAGE
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?
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x * 34000, recombinant ECHDC1, SDS-PAGE
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synthesis
improved artificial pathway for the biosynthesis of poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) with high 3-hydroxyhexanoate composition from fructose in Ralstonia eutropha. Introduction of EchDC1, encoding codon-optimized ethylmalonyl-CoA decarboxylase increases poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) biosynthesis, probably by converting ethylmalonyl-CoA generated by the reductive carboxylase activity of crotonyl-CoA carboxylase/reductase back into butyryl-CoA
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recombinant enzyme by metal affinity chromatography
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ECHDC1 from mouse brain cDNA, expression as N-terminally His-tagged enzyme
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Linster, C.L.; Noel, G.; Stroobant, V.; Vertommen, D.; Vincent, M.F.; Bommer, G.T.; Veiga-da-Cunha, M.; Van Schaftingen, E.
Ethylmalonyl-CoA decarboxylase, a new enzyme involved in metabolite proofreading
J. Biol. Chem.
286
42992-43003
2011
Homo sapiens, Mus musculus, Rattus norvegicus
brenda
Insomphun, C.; Xie, H.; Mifune, J.; Kawashima, Y.; Orita, I.; Nakamura, S.; Fukui, T.
Improved artificial pathway for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with high C6-monomer composition from fructose in Ralstonia eutropha
Metab. Eng.
27
38-45
2015
Mus musculus (Q9D9V3), Mus musculus
brenda