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The expected taxonomic range for this enzyme is: Glutamicibacter nicotianae
Synonyms
indole-3-carboxylate decarboxylase, more
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indole-3-carboxylate = indole + CO2
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indole-3-carboxylate carboxy-lyase
Activated by Zn2+, Mn2+ or Mg2+.
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2-methylindole + CO2
2-methylindole-3-carboxylate
indole-3-carboxylate
indole + CO2
2-methylindole + CO2
2-methylindole-3-carboxylate
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Substrates: -
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2-methylindole + CO2
2-methylindole-3-carboxylate
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Substrates: -
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indole-3-carboxylate
indole + CO2
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Substrates: -
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r
indole-3-carboxylate
indole + CO2
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Substrates: -
Products: -
r
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indole-3-carboxylate
indole + CO2
indole-3-carboxylate
indole + CO2
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Substrates: -
Products: -
r
indole-3-carboxylate
indole + CO2
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Substrates: -
Products: -
r
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additional information
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the enzyme does not need any cofactor for the decarboxylation and carboxylation reaction
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Ca2+
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1 mM, 1.3fold activation
Mg2+
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1 mM, 1.9fold activation
Mn2+
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1 mM, 2.1fold activation
Zn2+
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1 mM, 2.1fold activation
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1,10-phenanthroline
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1 mM, 85% inhibition
2,2'-bipyridyl
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1 mM, 72% inhibition
8-hydroxyquinoline
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1 mM, 49% inhibition
AgNO3
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1 mM, 50% inhibition
CuCl2
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1 mM, complete inhibition
HgCl2
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1 mM, 69% inhibition
hydroxylamine
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1 mM, 35% inhibition
N-ethylmaleimide
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1 mM, 97% inhibition
NaN3
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1 mM, 14% inhibition
phenylhydrazine
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1 mM, 87% inhibition
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0.719
indole
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pH 7.0, 30°C
0.631
indole-3-carboxylate
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pH 7.0, 30°C
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7
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decarboxylation, potassium phosphate buffer
8
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carboxylation, Tris-HCl buffer, 100 mM
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50
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decarboxylation. For the carboxylation reaction, temperatures over 30°C are not appropriate
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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30
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30 min, 13% loss of initial activity
40
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30 min, 35% loss of initial activity
50
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30 min, 65% loss of initial activity
60
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30 min, complete loss of initial activity
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4°C, cell extract, 80% of the initial activity is lost after 4 days
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induced specifically by indole-3-carboxylate, but not by indole
induced specifically by indole-3-carboxylate, but not by indole
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induced specifically by indole-3-carboxylate, but not by indole
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Yoshida, T.; Fujita, K.; Nagasawa, T.
Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation
Biosci. Biotechnol. Biochem.
66
2388-2394
2002
Glutamicibacter nicotianae, Glutamicibacter nicotianae FI1612
brenda
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