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Information on EC 4.1.1.68 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase for references in articles please use BRENDA:EC4.1.1.68
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EC Tree
IUBMB Comments Requires Mg2+ [2,3]. Part of the 4-hydroxyphenylacetate degradation pathway in Escherichia coli.
The enzyme appears in viruses and cellular organisms
Synonyms
2-Hydroxyhepta-2,4-diene-1,7-dioate isomerase/5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, 5-Oxopenta-3-ene-1,2,5-tricarboxylate decarboxylase, Decarboxylase, 5-oxopenta-3-ene-1,2,5-tricarboxylate, HHDD isomerase/OPET decarboxylase, HpcE, OPET decarboxylase,
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2-Hydroxyhepta-2,4-diene-1,7-dioate isomerase/5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
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5-Oxopenta-3-ene-1,2,5-tricarboxylate decarboxylase
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Decarboxylase, 5-oxopenta-3-ene-1,2,5-tricarboxylate
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HHDD isomerase/OPET decarboxylase
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OPET decarboxylase
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(2Z,4Z)-2-hydroxyhepta-2,4-dienedioate = (4Z)-2-oxohept-4-enedioate
(1b)
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(3E,5R)-5-carboxy-2-oxohept-3-enedioate = (2Z,4Z)-2-hydroxyhepta-2,4-dienedioate + CO2
(1a)
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(3E,5R)-5-carboxy-2-oxohept-3-enedioate = (4Z)-2-oxohept-4-enedioate + CO2
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decarboxylation
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5-oxopent-3-ene-1,2,5-tricarboxylate carboxy-lyase (2-oxohept-3-enedioate-forming)
Requires Mg2+ [2,3]. Part of the 4-hydroxyphenylacetate degradation pathway in Escherichia coli.
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151404-80-7
bifunctional enzyme ec4.1.1.68/ec5.3.3.10: isomerase, 2-hydroxyhepta-2,4-diene-1,7-dioate (Escherichia coli clone pFR835 gene hpcE reduced) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase/5-oxopent-2-ene-1,2,5-tricarboxylate decarboxylase (Escherichia coli C clone pDR835 gene hpcE) /decarboxylase, 5-oxopenta-3-ene-1,2,5-tricarboxylate (Escherichia coli clone pDR835 gene hpcE reduced)
173015-33-3
bifunctional enzyme ec4.1.1.68/ec5.3.3.10: isomerase, 2-hydroxyhepta-2,4-diene-1,7-dioate (Escherichia coli clone pHCB3 gene hpaG) /decarboxylase, 5-oxopenta-3-ene-1,2,5-tricarboxylate (Escherichia coli clone pHCB3 gene hpaG) /GenBank Z37980-derived protein
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5-carboxy-2-oxohept-3-enedioate + H+
2-hydroxyhepta-2,4-dienedioate + CO2
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enzyme is part of the 4-hydroxyphenylacetate meta-cleavage pathway
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5-Oxopent-3-ene-1,2,5-tricarboxylate
2-Oxohept-3-enedioate + CO2
5-Oxopent-3-ene-1,2,5-tricarboxylate
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enzyme is involved in the homoprotocatechuate degradative pathway
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5-Oxopent-3-ene-1,2,5-tricarboxylate
2-Oxohept-3-enedioate + CO2
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5-Oxopent-3-ene-1,2,5-tricarboxylate
2-Oxohept-3-enedioate + CO2
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5-Oxopent-3-ene-1,2,5-tricarboxylate
2-Oxohept-3-enedioate + CO2
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5-Oxopent-3-ene-1,2,5-tricarboxylate
2-Oxohept-3-enedioate + CO2
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5-Oxopent-3-ene-1,2,5-tricarboxylate
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enzyme is involved in the homoprotocatechuate degradative pathway
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0.032
5-oxopent-3-ene-1,2,5-tricarboxylate
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Uniprot
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; bifunctional enzyme with activity of EC 5.3.3.10 and EC 4.1.1.68
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bifunctional enzyme with activity of EC 5.3.3.10 and EC 4.1.1.68
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metabolism
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enzyme is part of the 4-hydroxyphenylacetate meta-cleavage pathway
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42100
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sedimentation equilibrium centrifugation
44514
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1 * 44514, calculation from nucleotide sequence
50000
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1 * 50000, SDS-PAGE
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dimer
crystallization experiments
monomer
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1 * 44514, calculation from nucleotide sequence; 1 * 50000, SDS-PAGE
monomer
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1 * 44514, calculation from nucleotide sequence; 1 * 50000, SDS-PAGE
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hanging drop vapor diffusion method
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4°C, stable for at least 2 months
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by ammonium sulfate precipitation and gel filtration, additional purification is achieved using DEAE-Sephacel chromatography
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Garrido-Pertierra, A.; Cooper, R.A.
Identification and purification of distinct isomerase and decarboxylase enzymes involved in the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli
Eur. J. Biochem.
117
581-584
1981
Escherichia coli
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Roper, D.I.; Cooper, R.A.
Purification, nucleotide sequence and some properties of a bifunctional isomerase/decarboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C
Eur. J. Biochem.
217
575-580
1993
Escherichia coli, Escherichia coli C
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Jervis, T.J.; Roper, D.I.; Glover, I.D.
Crystallization and preliminary X-ray analysis of a bifunctional enzyme: HHDD isomerase/OPET decarboxylase from Escherichia coli
Acta Crystallogr. Sect. D
52
1036-1038
1996
Escherichia coli
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Tame, J.R.; Namba, K.; Dodson, E.J.; Roper, D.I.
The crystal structure of HpcE, a bifunctional decarboxylase/isomerase with a multifunctional fold
Biochemistry
41
2982-2989
2002
Escherichia coli (P37352)
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