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Information on EC 4.1.1.61 - 4-hydroxybenzoate decarboxylase and Organism(s) Sedimentibacter hydroxybenzoicus and UniProt Accession Q4R101

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.61 4-hydroxybenzoate decarboxylase
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This record set is specific for:
Sedimentibacter hydroxybenzoicus
UNIPROT: Q4R101 not found.
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Word Map
The taxonomic range for the selected organisms is: Sedimentibacter hydroxybenzoicus
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
Synonyms
4-hydroxybenzoate decarboxylase, phenol carboxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Decarboxylase, p-hydroxybenzoate
-
-
-
-
p-Hydroxybenzoate decarboxylase
-
-
-
-
Phenol carboxylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzoate carboxy-lyase (phenol-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37290-53-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
?
Catechol + CO2
3,4-Dihydroxybenzoate
show the reaction diagram
-
-
-
?
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
minor stimulation of carboxylation of 4-hydroxybenzoate
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3,4-Trihydroxybenzoate
-
-
2,4,6-Trihydroxybenzoate
-
-
2,4-Dihydroxybenzoate
-
-
3,4,5-Trihydroxybenzoate
-
-
NH4+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Rb+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Zn2+
-
destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
3,4-dihydroxybenzoate
-
-
0.4
4-hydroxybenzoate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
18.3
3,4-dihydroxybenzoate
-
-
55
4-hydroxybenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
YCLC_SEDHY
480
0
53961
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
350000
-
gradient PAGE, gel filtration
57000
-
6 * 57000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
6 * 57000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.3
-
stable for 2 h
4740
6
-
11-40°C, stable for more than 24 h
4740
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11 - 40
-
pH 6.0, stable for more than 24 h
50
-
half-life: 12 h
56
-
half-life: 15 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive, 50% loss of activity when exposed to air at 5°C for 2 h
-
4740
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
He, Z.; Wiegel, J.
Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum
Eur. J. Biochem.
229
77-82
1995
Sedimentibacter hydroxybenzoicus
Manually annotated by BRENDA team
Huang, J.; He, Z.; Wiegel, J.
Cloning, characterization, and expression of a novel gene encoding a reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum
J. Bacteriol.
181
5119-5122
1999
Sedimentibacter hydroxybenzoicus (Q4R101 and Q9S4M7 and Q4R102), Sedimentibacter hydroxybenzoicus
Manually annotated by BRENDA team