We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 4.1.1.6 - cis-aconitate decarboxylase for references in articles please use BRENDA:EC4.1.1.6Word Map on EC 4.1.1.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The enzyme appears in viruses and cellular organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cis-aconitate decarboxylase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cis-aconitate = itaconate + CO2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
itaconate biosynthesis I
-
-
C5-Branched dibasic acid metabolism
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cis-aconitate carboxy-lyase (itaconate-forming)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Aconitate decarboxylase
-
-
-
-
cis-aconitate decarboxylase
cis-Aconitic acid decarboxylase
Decarboxylase, aconitate
-
-
-
-
CAD
-
-
CAD1
-
gene name
CadA
-
-
cis-aconitate decarboxylase
-
-
cis-aconitate decarboxylase
-
-
cis-aconitate decarboxylase
-
;
-
cis-Aconitic acid decarboxylase
-
-
-
-
cis-Aconitic acid decarboxylase
-
-
cis-Aconitic acid decarboxylase
-
-
cis-Aconitic acid decarboxylase
-
cis-Aconitic acid decarboxylase
-
-
-
cis-Aconitic acid decarboxylase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene cadA
-
-
brenda
-
UniProt
brenda
no activity in Ashbya gossypii
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
gene cadA
-
-
brenda
strains IFO6365 and TN484-M1
UniProt
brenda
-
-
-
brenda
gene cadA
-
-
brenda
-
UniProt
brenda
human gastric cancer cell lines SGC7901
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
-
the enzyme belongs to the MmgE/PrpD family
evolution
-
the enzyme belongs to the MmgE/PrpD family
-
metabolism
-
in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol
metabolism
-
in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol
metabolism
-
in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cis-aconitate
itaconate + CO2
citrate
itaconate + CO2
-
-
-
-
-
D-isocitrate
itaconate + CO2
-
-
-
-
-
cis-aconitate
?
-
enzyme is involved in biosynthesis of itaconate
-
-
-
cis-aconitate
?
-
key enzyme of itaconate biosynthesis
-
-
-
cis-aconitate
itaconate + CO2
-
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
-
cis-aconitate
itaconate + CO2
-
-
-
-
cis-aconitate
itaconate + CO2
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
?
cis-aconitate
itaconate + CO2
-
no activity with trans-aconitate
-
-
cis-aconitate
itaconate + CO2
-
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cis-aconitate
itaconate + CO2
cis-aconitate
?
-
enzyme is involved in biosynthesis of itaconate
-
-
-
cis-aconitate
?
-
key enzyme of itaconate biosynthesis
-
-
-
cis-aconitate
itaconate + CO2
-
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
-
?
cis-aconitate
itaconate + CO2
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Fluorocitrate
-
inhibits citrate decarboxylation, no inhibition of decarboxylation of cis-aconitate
L-Isocitric acid
-
competitive inhibition
p-chloromercuribenzoate
-
-
trans-aconitate
-
slight inhibition of decarboxylation of citrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.45
cis-aconitate
-
pH 6.2, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
a novel assay method
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.2
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
37
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
in human gastric cancer cell lines SGC7901
brenda
-
brenda
-
brenda
-
under conditions of itaconate production. Absent from growing mycelia
brenda
-
-
brenda
-
-
-
brenda
additional information
-
production of itaconic acid is achieved by the fermentation with Aspergillus terreus on sugar-containing media
brenda
additional information
-
production of itaconic acid is achieved by the fermentation with Aspergillus terreus on sugar-containing media
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
-
-
brenda
-
localized exclusively in cytosol
brenda
-
-
brenda
-
cytosolic isozyme
brenda
-
;
-
brenda
-
mitochondrial isozyme
brenda
-
brenda
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
52720
calculated from amino acid sequence
55000
SDS-PAGE
55000
SDS-PAGE, Flag-Irg1
55000
-
x * 55000, recombinant enzyme, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 55000, recombinant enzyme, SDS-PAGE
?
-
x * 55000, recombinant enzyme, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
80
-
recombinant enzyme in cell free extract, 20 min, inactivation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
no loss of activity after dialysis for 2 h at 0°C against 0.05 M phosphate buffer, pH 7. Inactivation after 15 h
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
espressed as recombinant protein in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells and in Aspergillus niger strain AB 1.13
-
expressed in Saccharomyces cerevisiae
gene cadA, heterologous expression in Aspergillus niger strain N201, expression of two previously identified Aspergillus terreus genes encoding putative organic acid transporters, genes mttA and mfsA, increases itaconic acid production in an Aspergillus niger cis-aconitate decarboxylase expressing strain, production method optimization, overview
-
gene cadA, heterologous functional expression in citric acid producing Aspergillus niger under various constitutive promoters of different expression strength, heterologous expression of the gene in Saccharomyces cerevisiae
-
gene cadA, the cadA gene of is located in a gene cluster together with two transporters, one a putative mitochondrial carrier, the other a putative plasma membrane carrier, cloning in Escherichia coli strain TOP10, heterologous mitochondrial and cytosolic expression of the enzyme in Aspergillus niger strain ATCC 1015, the mitochondrial expression doubles the enzyme productivity compared to the cytosolic expression. Co-expression of a cytosolic or a mitochondrial aconitase with cytosolic CadA leads to a significant increase of itaconic acid production compared to cytsolic CadA expression alone. The rate-limiting step for itaconic acid production under these conditions appears to be both the provision of cis-aconitate as a substrate for the CadA as well as the CAD activity itself
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
mutant strain which produces higher concentrations of itaconic acid is provoked by N-methyl N'-nitro-N-nitrosoguanidine treatment
additional information
-
a truncated version of the Aspergillus niger pfkA gene exhibits a higher citric acid yield due to a reduced inhibition by citrate and ATP, the truncated pfkA version has also a positive impact on the itaconic acid accumulation when expressed in Aspergillus terreus
additional information
-
a truncated version of the Aspergillus niger pfkA gene exhibits a higher citric acid yield due to a reduced inhibition by citrate and ATP, the truncated pfkA version has also a positive impact on the itaconic acid accumulation when expressed in Aspergillus terreus
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
purification with Ni-NTA resin under denaturing conditions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
industry
economic production of itaconic acid which is a supplement in many products
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IRG1_HUMAN
481
52628
Swiss-Prot
IRG1_MOUSE
488
53758
Swiss-Prot
CAD_ASPTE
490
52754
Swiss-Prot
CAD_ASPTN
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
490
52950
Swiss-Prot
A0A1M9ARK7_9MYCO
462
47468
TrEMBL
A0A173L1V1_9SPHN
476
49352
TrEMBL
A0A1M8WQ82_9MYCO
450
48132
TrEMBL
A0A1M8XCZ0_9MYCO
458
47998
TrEMBL
A0A1M9AI60_9MYCO
478
49922
TrEMBL
A0A173L2P7_9SPHN
483
52458
TrEMBL
A0A1M8XCJ7_9MYCO
461
48440
TrEMBL
A0A1M9H5V0_9MYCO
450
47798
TrEMBL
A0A1Y0X137_BACLI
445
47765
TrEMBL
A0A1M8G095_9MYCO
458
47413
TrEMBL
A0A1M9GEQ6_9MYCO
459
48193
TrEMBL
A0A1Y0XAS4_BACAM
448
47856
TrEMBL
A0A2S1ZAU5_ASPTE
490
52754
TrEMBL
I7GGR1_MYCS2
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
477
50880
TrEMBL
A0A1M8YQ03_9MYCO
458
48376
TrEMBL
A0A1M8S831_9MYCO
457
49228
TrEMBL
A0A1M8SHK4_9MYCO
464
48471
TrEMBL
A0A1M8MGX3_9MYCO
473
50605
TrEMBL
A0A1U3SFP4_9MYCO
448
47855
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Bentley, R.; Thiessen, C.P.
Biosynthesis of itaconic acid in Aspergillus terreus
J. Biol. Chem.
226
703-720
1957
Aspergillus terreus
brenda
Bonnarme, P.; Gillet, B.; Sepulchre, A.M.; Role, C.; Beloeil, J.C.; Ducrocq, C.
Itaconate biosynthesis in Aspergillus terreus
J. Bacteriol.
177
3573-3578
1995
Aspergillus terreus
brenda
Jaklitsch, W.M.; Kubicek, C.P.; Scrutton, M.C.
The subcellular organization of itaconate biosynthesis in Aspergillus terreus
J. Gen. Microbiol.
137
533-539
1991
Aspergillus terreus
-
brenda
Kanamasa, S.; Dwiarti, L.; Okabe, M.; Park, E.Y.
Cloning and functional characterization of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus
Appl. Microbiol. Biotechnol.
80
223-229
2008
Aspergillus terreus, Aspergillus terreus (B3IUN8)
brenda
Okabe, M.; Lies, D.; Kanamasa, S.; Park, E.Y.
Biotechnological production of itaconic acid and its biosynthesis in Aspergillus terreus
Appl. Microbiol. Biotechnol.
84
597-606
2009
Aspergillus terreus, Aspergillus terreus (B3IUN8)
brenda
Li, M.; Zhang, H.; Hong, L.; Jin, Y.; Gong, T.; Sun, L.; Yang, J.; Zheng, X.
Preparation and characterization of a novel monoclonal antibody specific to human CAD protein
Hybridoma
28
129-132
2009
Aspergillus terreus, Homo sapiens
brenda
Tevz, G.; Bencina, M.; Legisa, M.
Enhancing itaconic acid production by Aspergillus terreus
Appl. Microbiol. Biotechnol.
87
1657-1664
2010
Aspergillus terreus
brenda
Li, A.; van Luijk, N.; ter Beek, M.; Caspers, M.; Punt, P.; van der Werf, M.
A clone-based transcriptomics approach for the identification of genes relevant for itaconic acid production in Aspergillus
Fungal Genet. Biol.
48
602-611
2011
Aspergillus terreus, Aspergillus terreus NRRL1960
brenda
Michelucci, A.; Cordes, T.; Ghelfi, J.; Pailot, A.; Reiling, N.; Goldmann, O.; Binz, T.; Wegner, A.; Tallam, A.; Rausell, A.; Buttini, M.; Linster, C.L.; Medina, E.; Balling, R.; Hiller, K.
Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid production
Proc. Natl. Acad. Sci. USA
110
7820-7825
2013
Homo sapiens (A6NK06), Mus musculus (P54987)
brenda
Li, A.; Pfelzer, N.; Zuijderwijk, R.; Brickwedde, A.; van Zeijl, C.; Punt, P.
Reduced by-product formation and modified oxygen availability improve itaconic acid production in Aspergillus niger
Appl. Microbiol. Biotechnol.
97
3901-3911
2013
Aspergillus terreus
brenda
Steiger, M.G.; Blumhoff, M.L.; Mattanovich, D.; Sauer, M.
Biochemistry of microbial itaconic acid production
Front. Microbiol.
4
23
2013
Aspergillus itaconicus, Aspergillus terreus, Aspergillus terreus NRRL1960
brenda
Sugimoto, T.; Kato, T.; Park, E.Y.
Functional analysis of cis-aconitate decarboxylase and trans-aconitate metabolism in riboflavin-producing filamentous Ashbya gossypii
J. Biosci. Bioeng.
117
563-568
2014
no activity in Ashbya gossypii
brenda
Blumhoff, M.L.; Steiger, M.G.; Mattanovich, D.; Sauer, M.
Targeting enzymes to the right compartment: metabolic engineering for itaconic acid production by Aspergillus niger
Metab. Eng.
19
26-32
2013
Aspergillus terreus
brenda
html completed