Information on EC 4.1.1.52 - 6-methylsalicylate decarboxylase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
4.1.1.52
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RECOMMENDED NAME
GeneOntology No.
6-methylsalicylate decarboxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-methylsalicylate = 3-methylphenol + CO2
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
patulin biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
6-methylsalicylate carboxy-lyase (3-methylphenol-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37289-50-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Bromoorsellinic acid
1,3-Dihydroxy-2-bromo-5-methylbenzene + CO2
show the reaction diagram
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at 6% the activity with 6-methylsalicylic acid
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6-Ethylsalicylic acid
3-Ethyl-1-hydroxybenzene + CO2
show the reaction diagram
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at 100% of the activity with 6-methylsalicylic acid
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-
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6-methylsalicylate
3-methylphenol + CO2
show the reaction diagram
6-Methylsalicylate
?
show the reaction diagram
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enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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-
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6-Methylsalicylic acid
3-Cresol + CO2
show the reaction diagram
Homoorsellinic acid
?
show the reaction diagram
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at 100% of the activity with 6-methylsalicylic acid
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-
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Orsellinic acid
1,3-Dihydroxy-5-methylbenzene + CO2
show the reaction diagram
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at 80% of the activity with 6-methylsalicylic acid
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-methylsalicylate
3-methylphenol + CO2
show the reaction diagram
6-Methylsalicylate
?
show the reaction diagram
-
enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Hydroxy-1-naphthoic acid
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2-Methylbenzoic acid
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3-methylsalicylic acid
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4-methylsalicylic acid
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6-Methylanthranilic acid
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6-Methylsalicylic acid
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diethyl dicarbonate
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10 mM, complete inhibition
NaNO3
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10 mM, 20% inhibition
Rose bengal
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photooxidation
salicylic acid
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
3-bromoorsellinic acid
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-
0.01
6-Ethylsalicylic acid
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6-methylsalicylic acid
0.007
6-Methylsalicylic acid
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-
0.04
Homoorsellinic acid
-
-
0.1
orsellinic acid
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
grown in Czapek medium containing cycloheximide, no activity in rich germinating medium
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
x * 43000, V5 His-tagged enzyme, SDS-PAGE
56000 - 60000
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gel filtration, sucrose density gradient sedimentation
70000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
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unstable below
4701
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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5 min, unstable
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in solutions of low ionic strength
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in presence of rose Bengal is prevented in presence of substrate or 2-hydroxy-6-methylsalicylic acid
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4703
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, pH 7.5, 50% glycerol, 5% w/v (NH4)2SO4, stable for several months
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-25°C, pH 7.5, without glycerol, stable for at least 1 year
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
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LINKS TO OTHER DATABASES (specific for EC-Number 4.1.1.52)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)