Information on EC 4.1.1.52 - 6-methylsalicylate decarboxylase

for references in articles please use BRENDA:EC4.1.1.52
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
4.1.1.52
-
top print hide
RECOMMENDED NAME
GeneOntology No.
6-methylsalicylate decarboxylase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-methylsalicylate = 3-methylphenol + CO2
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
patulin biosynthesis
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
6-methylsalicylate carboxy-lyase (3-methylphenol-forming)
-
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
37289-50-2
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Bromoorsellinic acid
1,3-Dihydroxy-2-bromo-5-methylbenzene + CO2
show the reaction diagram
-
at 6% the activity with 6-methylsalicylic acid
-
-
-
6-Ethylsalicylic acid
3-Ethyl-1-hydroxybenzene + CO2
show the reaction diagram
-
at 100% of the activity with 6-methylsalicylic acid
-
-
-
6-methylsalicylate
3-methylphenol + CO2
show the reaction diagram
6-Methylsalicylate
?
show the reaction diagram
-
enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
-
-
-
6-Methylsalicylic acid
3-Cresol + CO2
show the reaction diagram
Homoorsellinic acid
?
show the reaction diagram
-
at 100% of the activity with 6-methylsalicylic acid
-
-
-
Orsellinic acid
1,3-Dihydroxy-5-methylbenzene + CO2
show the reaction diagram
-
at 80% of the activity with 6-methylsalicylic acid
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-methylsalicylate
3-methylphenol + CO2
show the reaction diagram
6-Methylsalicylate
?
show the reaction diagram
-
enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
-
-
-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Hydroxy-1-naphthoic acid
-
-
2-Methylbenzoic acid
-
-
3-methylsalicylic acid
-
-
4-methylsalicylic acid
-
-
6-Methylanthranilic acid
-
-
6-Methylsalicylic acid
-
-
diethyl dicarbonate
-
10 mM, complete inhibition
NaNO3
-
10 mM, 20% inhibition
Rose bengal
-
photooxidation
salicylic acid
-
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
3-bromoorsellinic acid
-
-
0.01
6-Ethylsalicylic acid
-
6-methylsalicylic acid
0.007
6-Methylsalicylic acid
-
-
0.04
Homoorsellinic acid
-
-
0.1
orsellinic acid
-
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
grown in Czapek medium containing cycloheximide, no activity in rich germinating medium
Manually annotated by BRENDA team
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
x * 43000, V5 His-tagged enzyme, SDS-PAGE
56000 - 60000
-
gel filtration, sucrose density gradient sedimentation
70000
-
gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
unstable below
4701
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
5 min, unstable
top print hide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in solutions of low ionic strength
-
top print hide
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in presence of rose Bengal is prevented in presence of substrate or 2-hydroxy-6-methylsalicylic acid
-
4703
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, pH 7.5, 50% glycerol, 5% w/v (NH4)2SO4, stable for several months
-
-25°C, pH 7.5, without glycerol, stable for at least 1 year
-
top print hide
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 4.1.1.52)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)