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Information on EC 4.1.1.52 - 6-methylsalicylate decarboxylase
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4.1.1.52 6-methylsalicylate decarboxylaseSpecify your search results
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The enzyme appears in viruses and cellular organisms
Synonyms
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase, 6-Methylsalicylic acid decarboxylase, 6-MSA decarboxylase, AOL_s00215g281, Decarboxylase, 6-methylsalicylate, PatG, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase
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-
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6-Methylsalicylic acid decarboxylase
6-MSA decarboxylase
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-
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AOL_s00215g281
Decarboxylase, 6-methylsalicylate
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PatG
6-Methylsalicylic acid decarboxylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-methylsalicylate = 3-methylphenol + CO2
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decarboxylation
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PATHWAY SOURCE
PATHWAYS
MetaCyc
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SYSTEMATIC NAME
IUBMB Comments
6-methylsalicylate carboxy-lyase (3-methylphenol-forming)
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CAS REGISTRY NUMBER
COMMENTARY
37289-50-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-Bromoorsellinic acid
1,3-Dihydroxy-2-bromo-5-methylbenzene + CO2
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at 6% the activity with 6-methylsalicylic acid
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?
6-Ethylsalicylic acid
3-Ethyl-1-hydroxybenzene + CO2
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at 100% of the activity with 6-methylsalicylic acid
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?
6-Methylsalicylate
?
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enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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?
Homoorsellinic acid
?
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at 100% of the activity with 6-methylsalicylic acid
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?
Orsellinic acid
1,3-Dihydroxy-5-methylbenzene + CO2
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at 80% of the activity with 6-methylsalicylic acid
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-Methylsalicylate
?
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enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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grown in Czapek medium containing cycloheximide, no activity in rich germinating medium
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
a mutant lacking the enzyme activity shows huge accumulation of 6-methylsalicylic acid in the cultural broth. 6-methylsalicylic acid and m-cresol display significant nematicidal activity toward root-knot nematodes Meloidogyne incognita
physiological function
Orbilia oligospora DSM 1491
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a mutant lacking the enzyme activity shows huge accumulation of 6-methylsalicylic acid in the cultural broth. 6-methylsalicylic acid and m-cresol display significant nematicidal activity toward root-knot nematodes Meloidogyne incognita
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ACUB_ASPA1
Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094)
330
0
36231
Swiss-Prot
other Location (Reliability: 2)
YANB_ASPNA
Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7)
368
0
40345
Swiss-Prot
other Location (Reliability: 2)
AR281_ARTOA
Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491)
325
0
36066
Swiss-Prot
other Location (Reliability: 2)
PATG_ASPCL
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
325
0
36217
Swiss-Prot
other Location (Reliability: 2)
PATG_PENEN
324
0
35653
Swiss-Prot
other Location (Reliability: 3)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
56000 - 60000
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gel filtration, sucrose density gradient sedimentation
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in presence of rose Bengal is prevented in presence of substrate or 2-hydroxy-6-methylsalicylic acid
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4703
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, pH 7.5, 50% glycerol, 5% w/v (NH4)2SO4, stable for several months
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
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when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
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biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol
synthesis
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biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Light, R.J.
6-Methylsalicylic acid decarboxylase from Penicillium patulum
Biochim. Biophys. Acta
191
430-438
1969
Penicillium griseofulvum
brenda
Slater, G.P.; Child, J.J.; Hogge, L.R.
The decarboxylation of 6-methylsalicylic acid by Valsa friesii
Can. J. Microbiol.
19
1169-1171
1973
Valsa friesii
brenda
Light, R.J.; Vogel, G.
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase
Methods Enzymol.
43
530-540
1975
Penicillium griseofulvum
brenda
Snini, S.P.; Tadrist, S.; Laffitte, J.; Jamin, E.L.; Oswald, I.P.; Puel, O.
The gene PatG involved in the biosynthesis pathway of patulin, a food-borne mycotoxin, encodes a 6-methylsalicylic acid decarboxylase
Int. J. Food Microbiol.
171
77-83
2014
Aspergillus clavatus (T1PRE6), Aspergillus clavatus, Aspergillus clavatus NRRL 1980 (T1PRE6)
brenda
Xu, Z.; Chen, Y.; Song, T.; Zeng, Z.; Yan, N.; Zhang, K.; Niu, X.
Nematicidal key precursors for the biosynthesis of morphological regulatory arthrosporols in the nematode-trapping fungus Arthrobotrys oligospora
J. Agric. Food Chem.
64
7949-7956
2016
Orbilia oligospora (G1XU02), Orbilia oligospora DSM 1491 (G1XU02)
brenda
Hitschler, J.; Boles, E.
De novo production of aromatic m-cresol in Saccharomyces cerevisiae mediated by heterologous polyketide synthases combined with a 6-methylsalicylic acid decarboxylase
Metab. Eng. Commun.
9
e00093
2019
Aspergillus clavatus
brenda
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