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Information on EC 4.1.1.52 - 6-methylsalicylate decarboxylase
for references in articles please use BRENDA:EC4.1.1.52
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EC Tree 4 Lyases
4.1 Carbon-carbon lyases
4.1.1 Carboxy-lyases
4.1.1.52 6-methylsalicylate decarboxylase
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
showSynonyms
6-msa decarboxylase, 6-methylsalicylic acid decarboxylase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase
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6-Methylsalicylic acid decarboxylase
6-MSA decarboxylase
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-
-
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AOL_s00215g281
Decarboxylase, 6-methylsalicylate
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PatG
6-Methylsalicylic acid decarboxylase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-methylsalicylate = 3-methylphenol + CO2
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decarboxylation
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PATHWAY SOURCE
PATHWAYS
MetaCyc
patulin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
6-methylsalicylate carboxy-lyase (3-methylphenol-forming)
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CAS REGISTRY NUMBER
COMMENTARY
37289-50-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-Bromoorsellinic acid
1,3-Dihydroxy-2-bromo-5-methylbenzene + CO2
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Substrates: at 6% the activity with 6-methylsalicylic acid
Products: -
Products: -
?
6-Ethylsalicylic acid
3-Ethyl-1-hydroxybenzene + CO2
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Substrates: at 100% of the activity with 6-methylsalicylic acid
Products: -
Products: -
?
6-Methylsalicylate
?
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Substrates: enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
Products: -
Products: -
?
6-Methylsalicylic acid
3-Cresol + CO2
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Substrates: -
Products: -
Products: -
?
Homoorsellinic acid
?
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Substrates: at 100% of the activity with 6-methylsalicylic acid
Products: -
Products: -
?
Orsellinic acid
1,3-Dihydroxy-5-methylbenzene + CO2
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Substrates: at 80% of the activity with 6-methylsalicylic acid
Products: -
Products: -
?
6-methylsalicylate
3-methylphenol + CO2
Substrates: -
Products: -
Products: -
?
6-methylsalicylate
3-methylphenol + CO2
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-Methylsalicylate
?
-
Substrates: enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
Products: -
Products: -
?
6-methylsalicylate
3-methylphenol + CO2
Substrates: -
Products: -
Products: -
?
6-methylsalicylate
3-methylphenol + CO2
Substrates: -
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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grown in Czapek medium containing cycloheximide, no activity in rich germinating medium
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
a mutant lacking the enzyme activity shows huge accumulation of 6-methylsalicylic acid in the cultural broth. 6-methylsalicylic acid and m-cresol display significant nematicidal activity toward root-knot nematodes Meloidogyne incognita
physiological function
Orbilia oligospora DSM 1491
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a mutant lacking the enzyme activity shows huge accumulation of 6-methylsalicylic acid in the cultural broth. 6-methylsalicylic acid and m-cresol display significant nematicidal activity toward root-knot nematodes Meloidogyne incognita
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Show AA Sequence and Transmembrane Helices (2094 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
56000 - 60000
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gel filtration, sucrose density gradient sedimentation
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in presence of rose Bengal is prevented in presence of substrate or 2-hydroxy-6-methylsalicylic acid
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4703
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, pH 7.5, 50% glycerol, 5% w/v (NH4)2SO4, stable for several months
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
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when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
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biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol
synthesis
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biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Light, R.J.
6-Methylsalicylic acid decarboxylase from Penicillium patulum
Biochim. Biophys. Acta
191
430-438
1969
Penicillium griseofulvum
brenda
Slater, G.P.; Child, J.J.; Hogge, L.R.
The decarboxylation of 6-methylsalicylic acid by Valsa friesii
Can. J. Microbiol.
19
1169-1171
1973
Cytospora pinastri
brenda
Light, R.J.; Vogel, G.
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase
Methods Enzymol.
43
530-540
1975
Penicillium griseofulvum
brenda
Snini, S.P.; Tadrist, S.; Laffitte, J.; Jamin, E.L.; Oswald, I.P.; Puel, O.
The gene PatG involved in the biosynthesis pathway of patulin, a food-borne mycotoxin, encodes a 6-methylsalicylic acid decarboxylase
Int. J. Food Microbiol.
171
77-83
2014
Aspergillus clavatus (T1PRE6), Aspergillus clavatus, Aspergillus clavatus NRRL 1980 (T1PRE6)
brenda
Xu, Z.; Chen, Y.; Song, T.; Zeng, Z.; Yan, N.; Zhang, K.; Niu, X.
Nematicidal key precursors for the biosynthesis of morphological regulatory arthrosporols in the nematode-trapping fungus Arthrobotrys oligospora
J. Agric. Food Chem.
64
7949-7956
2016
Orbilia oligospora (G1XU02), Orbilia oligospora DSM 1491 (G1XU02)
brenda
Hitschler, J.; Boles, E.
De novo production of aromatic m-cresol in Saccharomyces cerevisiae mediated by heterologous polyketide synthases combined with a 6-methylsalicylic acid decarboxylase
Metab. Eng. Commun.
9
e00093
2019
Aspergillus clavatus
brenda
EXTERNAL LINKS (specific for EC-Number 4.1.1.52)
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