Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 4.1.1.50 - adenosylmethionine decarboxylase and Organism(s) Datura stramonium and UniProt Accession Q96555

for references in articles please use BRENDA:EC4.1.1.50
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.50 adenosylmethionine decarboxylase
IUBMB Comments
The Escherichia coli enzyme contains a pyruvoyl group.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Datura stramonium
UNIPROT: Q96555
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Datura stramonium
The enzyme appears in selected viruses and cellular organisms
Synonyms
s-adenosylmethionine decarboxylase, adometdc, samdc, s-adenosyl-l-methionine decarboxylase, adenosylmethionine decarboxylase, adomet decarboxylase, s-adenosyl methionine decarboxylase, sam-dc, sam decarboxylase, pfadometdc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S-Adenosylmethionine decarboxylase
-
AdoMetDC
-
-
-
-
AMDC
-
-
-
-
S-Adenosyl-L-methionine decarboxylase
-
-
-
-
S-Adenosylmethionine decarboxylase
SAM decarboxylase
SAMDC
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine carboxy-lyase [(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium-salt-forming]
The Escherichia coli enzyme contains a pyruvoyl group.
CAS REGISTRY NUMBER
COMMENTARY hide
9036-20-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine
(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine
(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DCAM_DATST
362
0
39956
Swiss-Prot
other Location (Reliability: 2)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA of Datura stramonium is introduced in Nicotiana tabacum L. cv. Xanthi in antisense orientation under the CaMV 35S promoter, by means of Agrobacterium tumefaciens and leaf disc transformation. Primary transformants efficiently transcribe the antisense SAMDC gene, but SAMDC activity and PA titers do not change. By contrast, in most transgenic shoots of the second generation, SAMDC activity is remarkably lower than in controls, and the putrescine-to-spermidine ratio is altered, mainly due to increased putrescine, even though putrescine oxidising activity (diamine oxidase, EC 1.4.3.6) does not change relative to controls. Despite the reduction in SAMDC activity, the production of ethylene, which shares with PAs the common precursor SAM, is not influenced by the foreign gene. Some plants are transferred to pots and acclimatised in a growth chamber. In these in vivo-grown second generation transgenic plants, at the vegetative stage, SAMDC activity is scarcely reduced, and PA titres do not change. Finally, the rhizogenic potential of in vitro-cultured leaf explants excised from antisense plants is significantly diminished as compared with WTones, and the response to methyl jasmonate, a stress-mimicking compound, in terms of PA conjugation, is higher and differentially affected in transgenic leaf discs relative to WT ones
expressed in Oryza sativa subspecies Japonica cultivar EYI105
expressed in Oryza sativa
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the levels of SAMDC1 mRNA are reduced after 3 d of drought stress, but after 6 d of stress and throughout the recovery period, steady-state SAMDC1 mRNA levels return to normal
exogenous methyl jasmonate transiently inhibits the expression of Samdc gene in the polyamine biosynthesis pathway, the level of Samdc1 mRNA falls by 50% after 6 h exposure to 0.2 mM methyl jasmonate, but returns to near initial levels after 48 h
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
overexpression of enzyme is sufficient for accumulation of spermidine in leaves and spermidine and spermine in seeds
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thu-Hang, P.; Bassie, L.; Safwat, G.; Trung-Nghia, P.; Christou, P.; Capell, T.
Expression of a heterologous S-adenosylmethionine decarboxylase cDNA in plants demonstrates that changes in S-adenosyl-L-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine
Plant Physiol.
129
1744-1754
2002
Datura stramonium (Q96555), Datura stramonium
Manually annotated by BRENDA team
Torrigiani, P.; Scaramagli, S.; Ziosi, V.; Mayer, M.; Biondi, S.
Expression of an antisense Datura stramonium S-adenosylmethionine decarboxylase cDNA in tobacco: changes in enzyme activity, putrescine-spermidine ratio, rhizogenic potential, and response to methyl jasmonate
J. Plant Physiol.
162
559-571
2005
Datura stramonium (Q96555), Datura stramonium
Manually annotated by BRENDA team
Peremarti, A.; Bassie, L.; Christou, P.; Capell, T.
Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase
Plant Mol. Biol.
70
253-264
2009
Oryza sativa Japonica Group (Q0JC10), Datura stramonium (Q96555), Datura stramonium
Manually annotated by BRENDA team
Peremarti, A.; Bassie, L.; Yuan, D.; Pelacho, A.; Christou, P.; Capell, T.
Transcriptional regulation of the rice arginine decarboxylase (Adc1) and S-adenosylmethionine decarboxylase (Samdc) genes by methyl jasmonate
Plant Physiol. Biochem.
48
553-559
2010
Datura stramonium
Manually annotated by BRENDA team