Information on EC 4.1.1.44 - 4-carboxymuconolactone decarboxylase

for references in articles please use BRENDA:EC4.1.1.44
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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
4.1.1.44
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RECOMMENDED NAME
GeneOntology No.
4-carboxymuconolactone decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 4,5-dihydro-5-oxofuran-2-acetate + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
protocatechuate degradation II (ortho-cleavage pathway)
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4-hydroxymandelate degradation
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Benzoate degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate carboxy-lyase (4,5-dihydro-5-oxofuran-2-acetate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37289-46-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
and strains Y3F, Y4I
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
4-carboxymuconolactone
?
show the reaction diagram
4-carboxymuconolactone
beta-oxoadipate enol-lactone + ?
show the reaction diagram
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?
gamma-carboxymuconolactone
beta-ketoadipate enol-lactone + ?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
4-carboxymuconolactone
?
show the reaction diagram
4-carboxymuconolactone
beta-oxoadipate enol-lactone + ?
show the reaction diagram
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.82
4-carboxymuconolactone
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15950
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caculated value
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method, 22%(w/v) polyethylene glycol 3350, 0.2 M sodium chloride, 0.1 M Na HEPES pH 7.0 and 10%(v/v) glycerol, the crystal belongs to space group P43212, with unit-cell parameters a = b = 66.66, c = 184.82 A, resolution 2.40 A, the Matthews coefficient and solvent content are estimated to be 2.14 A3/Da and 42.6%, respectively, assuming that the asymmetric unit contains three recombinant protein molecules
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
centrifugal ultrafiltration, gel filtration
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copurification of 3-oxoadipate enol-lactone-hydrolyzing activity, ec 3.1.1.24 and 4-carboxymuconolactone-decarboxylating activity, ec 4.1.1.44
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
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overexpression in Escherichia coli strain Rosetta 2 (DE3)
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