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Information on EC 4.1.1.4 - acetoacetate decarboxylase and Organism(s) Clostridium beijerinckii and UniProt Accession A6M020

for references in articles please use BRENDA:EC4.1.1.4
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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.4 acetoacetate decarboxylase
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Select one or more organisms in this record: ?
This record set is specific for:
Clostridium beijerinckii
UNIPROT: A6M020 not found.
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Word Map
The taxonomic range for the selected organisms is: Clostridium beijerinckii
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
acetoacetate decarboxylase, aadase, acetoacetic acid decarboxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AAD
-
-
-
-
acetoacetic acid decarboxylase
-
-
Acetoacetic decarboxylase
-
-
-
-
ADC
-
-
-
-
CP 28/CP 29
-
-
-
-
Decarboxylase, acetoacetate
-
-
-
-
Polymyxin MI
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acetoacetate carboxy-lyase (acetone-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-03-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetate + H+
acetone + CO2
show the reaction diagram
Acetoacetate
?
show the reaction diagram
-
the enzyme is involved in the metabolic pathway of isopropanol
-
-
?
Acetoacetate
Acetone + CO2
show the reaction diagram
-
-
-
?
acetoacetate + H+
acetone + CO2
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetoacetate + H+
acetone + CO2
show the reaction diagram
-
-
-
?
Acetoacetate
?
show the reaction diagram
-
the enzyme is involved in the metabolic pathway of isopropanol
-
-
?
acetoacetate + H+
acetone + CO2
show the reaction diagram
-
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
in pH-controlled cultures, the addition of 20 mM acetate promotes a more vigorous solventogenic fermentation
Butyrate
-
in pH-controlled cultures, the addition of 20 mM acetate promotes a more vigorous solventogenic fermentation
spo0A
-
in gel retardation assays, the C-terminal domains of Spo0A is able to bind fragments of acetoacetate decarboxylase promoter, Spo0A directly controls the shift between acidogenesis and solventogenesis, insertional inactivation of spo0A blocked the formation of solvents
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene aadc
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme AADC-catalyzed decarboxylation of acetoacetate is not the sole means for acetone generation
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
generation of an aadc deletion mutant, the mutant produces a maximum acetone concentration comparable to that produced by wild-type. Non-enzymatic decarboxylation of acetoacetate in vitro, under conditions similar to in vivo acetone-butanol-ethanol fermentation, produces 1.3 to 5.2 g/L acetone between pH 4.0-6.5 and explains why various knock-out and knockdown strategies designed to disrupt aadc in solventogenic Clostridium species do not eliminate acetone production during acetone-butanol-ethanol fermentation
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene aadc encoded in the sol operon, cloning of gene fragments aadc358-1 and aadc358-2 in Escherichia coli strain DH5alpha, expression as GFP-tagged enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yan, R.T.; Zhu, C.X.; Golemboski, C.; Chen, J.S.
Expression of solvent-forming enzymes and onset of solvent production in batch cultures of Clostridium beijerinckii
Appl. Environ. Microbiol.
54
642-648
1988
Clostridium beijerinckii
Manually annotated by BRENDA team
Ravagnani, A.; Jennert, K.C.B.; Steiner, E.; Grunberg, R.; Jefferies, J.R.; Wilkinson, S.R.; Young, D.I.; Tidswell, E.C.; Brown, D.P.; Youngman, P.; Morris, J.G.; Young M.
Spo0A directly controls the switch from acid to solvent production in solvent-forming clostridia
Mol. Microbiol.
37
1172-1185
2000
Clostridium acetobutylicum, Clostridium beijerinckii, Clostridium beijerinckii AA243, Clostridium beijerinckii NCIMB 8052, Ruminiclostridium cellulolyticum
Manually annotated by BRENDA team
Han, B.; Gopalan, V.; Ezeji, T.C.
Acetone production in solventogenic Clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate
Appl. Microbiol. Biotechnol.
91
565-576
2011
Clostridium acetobutylicum (P23670), Clostridium beijerinckii (A6M020), Clostridium beijerinckii NCIMB 8052 (A6M020), Clostridium beijerinckii NCIMB 8052
Manually annotated by BRENDA team