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Information on EC 4.1.1.33 - diphosphomevalonate decarboxylase and Organism(s) Rattus norvegicus and UniProt Accession Q62967
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4.1.1.33 diphosphomevalonate decarboxylaseSpecify your search results
Word Map
- 4.1.1.33
-
isoprenoids
- mevalonate-5-phosphate
-
cholesterogenesis
-
6-fluoromevalonate
- cholestyramine
-
polyisoprenoid
- 5-phosphomevalonate
- synthesis
- biofuel production
- pharmacology
- drug development
- medicine
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mevalonate diphosphate decarboxylase, mevalonate pyrophosphate decarboxylase, diphosphomevalonate decarboxylase, mevalonate-5-pyrophosphate decarboxylase, mevalonate 5-diphosphate decarboxylase, pyrophosphomevalonate decarboxylase, scmdd, 5-pyrophosphomevalonate decarboxylase, scmdd1, scmdd2, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
5-Pyrophosphomevalonate decarboxylase
-
-
-
-
Decarboxylase, pyrophosphomevalonate
-
-
-
-
Mevalonate (diphospho)decarboxylase
-
-
-
-
Mevalonate 5-diphosphate decarboxylase
Mevalonate pyrophosphate decarboxylase
-
-
-
-
Mevalonate-5-pyrophosphate decarboxylase
Pyrophosphomevalonate decarboxylase
-
-
-
-
Pyrophosphomevalonic acid decarboxylase
-
-
-
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Mevalonate 5-diphosphate decarboxylase
-
-
-
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Mevalonate-5-pyrophosphate decarboxylase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-5-diphosphomevalonate carboxy-lyase (adding ATP; isopentenyl-diphosphate-forming)
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CAS REGISTRY NUMBER
COMMENTARY
9024-66-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
enzyme activity is increased by 5fold after feeding young rats 2.5% cholestyramine for ten days to four weeks
-
-
?
additional information
?
-
-
major enzyme involved in cholesterol biosynthesis in rat liver
-
?
additional information
?
-
-
the enzyme is involved in cholesterol biosynthesis
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
-
enzyme activity is increased by 5fold after feeding young rats 2.5% cholestyramine for ten days to four weeks
-
-
?
additional information
?
-
-
major enzyme involved in cholesterol biosynthesis in rat liver
-
?
additional information
?
-
-
the enzyme is involved in cholesterol biosynthesis
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2E)-3,7-dimethylocta-2,6-dien-1-yl 2-(4-hydroxy-4-methyl-6-oxotetrahydro-2H-pyran-2-yl)ethyl diphosphate
-
-
(2E)-3,7-dimethylocta-2,6-dien-1-yl 4-(4-hydroxy-4-methyl-6-oxotetrahydro-2H-pyran-2-yl)butyl diphosphate
-
-
additional information
-
probucol decreases mevalonate pyrophosphate decarboxylase in the rat liver
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00302
2-fluoromevalonate 5-diphosphate
-
in 100 mM Tris buffer, pH 7.5, at 25°C
0.0046
P'-geranyl 2-fluoromevalonate 5-diphosphate
-
in 100 mM Tris buffer, pH 7.5, at 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0026
(2E)-3,7-dimethylocta-2,6-dien-1-yl 2-(4-hydroxy-4-methyl-6-oxotetrahydro-2H-pyran-2-yl)ethyl diphosphate
Rattus norvegicus
-
-
0.0065
(2E)-3,7-dimethylocta-2,6-dien-1-yl 4-(4-hydroxy-4-methyl-6-oxotetrahydro-2H-pyran-2-yl)butyl diphosphate
Rattus norvegicus
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 9
-
about 35% of maximal activity at pH 4 and at pH 9, 37000 MW enzyme form
6 - 9
-
pH 6: about 35% of maximal activity, pH 9: about 30% of maximal activity, 45000 MW enzyme form
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
decrease in activity when rats are fasted, fed with a diet containing 2% cholesterol or made diabetic, increase in activity when the animals are fed with a diet containing 3% cholestyramine
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lower activity of mevalonate-diphosphate decarboxylase is responsible for the reduced cholesterol biosynthesis in the liver of stroke-prone spontaneously hypertensive rat
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
contains a small amount of the 45000 Da enzyme species, but not the 37000 Da species. The 45000 Da enzyme species is localized in the matrix
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MVD1_RAT
401
0
43893
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37000
-
2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE
45000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
additional information
-
a diet containing 5% cholestyramine and 0.1% pravastatin induces a new enzyme species of 37000 Da
dimer
-
2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
intracellular glycosylation does not contribute to the difference between the 45000 MW form and the 37000 MW form of the enzyme
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -73°C, rat liver homogenate with 0.1 mM leupeptin, 0.75 mg/l aprotinin, 0.1 mM phenylmethylsulfonyl fluoride, 15 mM EDTA, 15 mM EGTA, stable for over 3 months
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms: MW 37000 and MW 45000. The MW 37000 enzyme form is induced by the diet containing cholestyramine and pravastin
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
MPD mRNA expression in liver and MPD protein in brain of stroke-prone spontaneously hypertensive rats is significantly reduced
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
the upregulation of microRNA-214 and downregulation of mevalonate diphosphate decarboxylase transcription in the liver both play a role in the development of hypocholesterolemia in stroke-prone spontaneously hypertensive rats
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jabalquinto, A.M.; Cardemil, E.
The effect of diabetes, nutritional factors, and sex on rat liver and kidney mevalonate kinase, mevalonate-5-phosphate kinase, and mevalonate-5-pyrophosphate decarboxylase
Arch. Biochem. Biophys.
210
132-139
1981
Rattus norvegicus
Toth, M.J.; Huwyler, L.; Park, J.
Purification of rat liver mevalonate pyrophosphate decarboxylase
Prep. Biochem. Biotechnol.
26
47-51
1996
Rattus norvegicus
Michihara, A.; Sawamura, M.; Nara, Y.; Ikeda, K.; Yamori, Y.
Purification and characterization of two mevalonate pyrophosphate decarboxylases from rat liver: a novel molecular species of 37 kDa
J. Biochem.
122
647-654
1997
Rattus norvegicus
Hulcher, F.H.
Modification of the radiochemical assay of rat liver mevalonate-5-diphosphate decarboxylase and induction and stabilization of the activity
Biochem. Biophys. Res. Commun.
181
1449-1455
1991
Rattus norvegicus
Sawamura, M.; Nara, Y.; Yamori, Y.
Liver mevalonate 5-pyrophosphate decarboxylase is responsible for reduced serum cholesterol in stroke-prone spontaneously hypertensive rat
J. Biol. Chem.
267
6051-6055
1992
Rattus norvegicus
Michihara, A.; Akasaki, K.; Yamori, Y.; Tsuji, H.
Tissue distribution of a major mevalonate pyrophosphate decarboxylase in rats
Biol. Pharm. Bull.
24
1231-1234
2001
Rattus norvegicus
Michihara, A.; Sawamura, M.; Yamori, Y.; Akasaki, K.; Tsuji, H.
Mevalonate pyrophosphate decarboxylase is predominantly located in the cytosol of rat hepatocytes
Biol. Pharm. Bull.
24
1235-1240
2001
Rattus norvegicus
Michihara, A.; Sawamura, M.; Yamori, Y.; Akasaki, K.; Tsuji, H.
Difference in subcellular distribution between 45- and 37-kDa mevalonate pyrophosphate decarboxylase in rat liver
Biol. Pharm. Bull.
24
1347-1350
2001
Rattus norvegicus
Michihara, A.; Sawamura, M.; Yamori, Y.; Akasaki, K.; Tsuji, H.
Probucol decreases mevalonate pyrophosphate decarboxylase in the rat liver
Biol. Pharm. Bull.
26
1484-1486
2003
Rattus norvegicus
Qiu, Y.; Li, D.
Bifunctional inhibitors of mevalonate kinase and mevalonate 5-diphosphate decarboxylase
Org. Lett.
8
1013-1016
2006
Rattus norvegicus
Qiu, Y.; Li, D.
Inhibition of mevalonate 5-diphosphate decarboxylase by fluorinated substrate analogs
Biochim. Biophys. Acta
1760
1080-1087
2006
Rattus norvegicus
Qiu, Y.; Gao, J.; Guo, F.; Qiao, Y.; Li, D.
Mutation and inhibition studies of mevalonate 5-diphosphate decarboxylase
Bioorg. Med. Chem. Lett.
17
6164-6168
2007
Rattus norvegicus
Wang, Y.; Muneton, S.; Sjoevall, J.; Jovanovic, J.N.; Griffiths, W.J.
The effect of 24S-hydroxycholesterol on cholesterol homeostasis in neurons: quantitative changes to the cortical neuron proteome
J. Proteome Res.
7
1606-1614
2008
Rattus norvegicus
Michihara, A.; Shimatani, M.; Akasaki, K.
Comparison of the gene expression levels of mevalonate pyrophosphate decarboxylase between stroke-prone spontaneously hypertensive and Wistar Kyoto rats
J. Health Sci.
56
733-737
2010
Rattus norvegicus (Q62967)
-
Michihara, A.; Ide, N.; Mizutani, Y.; Okamoto, M.; Uchida, M.; Matsuoka, H.; Akasaki, K.
Involvement of microRNA214 and transcriptional regulation in reductions in mevalonate pyrophosphate decarboxylase mRNA levels in stroke-prone spontaneously hypertensive rat livers
Biosci. Biotechnol. Biochem.
79
1759-1770
2015
Rattus norvegicus (Q62967)
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