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Information on EC 3.8.1.8 - atrazine chlorohydrolase
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3.8.1.8 atrazine chlorohydrolaseIUBMB Comments
Involved in the degradation of the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, in bacteria.
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Word Map
- 3.8.1.8
-
herbicide
- adp
-
dechlorination
- hydroxyatrazine
- arthrobacter
- s-triazine
-
amidohydrolase
- nocardioides
- melamine
-
atrazine-degrading
-
bioremediation
- agriculture
- haematococcus
- ametryn
-
cyanuric
- aurescens
-
phytoremediation
- analysis
- environmental protection
- biotechnology
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
atrazine chlorohydrolase, triazine hydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
atrazine chlorohydrolase
atrazine chlorohydrolase 2
atrazine dechlorinase
-
-
-
-
AtzA
dechlorinase, atrazine (9CI)
-
-
-
-
triazine hydrolase
TrZN
additional information
-
enzyme shows 98% amino acid sequence identitiy with melamine deaminase from Pseudomonas sp. strain NRRL B-12227, but does not catalyse the same reaction
AtzA
Clavibacter michiganese ATZ1
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
atrazine + H2O = hydroxyatrazine + chloride
-
-
-
-
atrazine + H2O = hydroxyatrazine + chloride
catalytic mechanism, the Glu241 side chain provides a proton to N-1 of the s-triazine substrate to facilitate nucleophilic displacement at the adjacent C-2, overview
-
atrazine + H2O = hydroxyatrazine + chloride
two plausible reaction mechanisms are proposed involving either bidentate (a) or mondentate (b) coordination of the atrazine Cl atom to the Fe2+ centre of the AtzA active site, comparisons, overview
atrazine + H2O = hydroxyatrazine + chloride
catalytic mechanism, the Glu241 side chain provides a proton to N-1 of the s-triazine substrate to facilitate nucleophilic displacement at the adjacent C-2, overview
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
atrazine chlorohydrolase
Involved in the degradation of the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, in bacteria.
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CAS REGISTRY NUMBER
COMMENTARY
168680-16-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O
6-[(2-methylpropyl)amino]-1,3,5-triazine-2,4-diol + isobutylamine
-
-
-
-
?
2,4-di(N-secbutylamino)-6-hydroxy-1,3,5-triazine + H2O
6-[(1-methylpropyl)amino]-1,3,5-triazine-2,4-diol + butan-2-amine
-
-
-
-
?
2,4-di(N-tertbutylamino)-6-hydroxy-1,3,5-triazine + H2O
6-(tert-butylamino)-1,3,5-triazine-2,4-diol + tert-butylamine
-
-
-
-
?
2,4-diamino-6-hydroxy-1,3,5-triazine + H2O
6-amino-1,3,5-triazine-2,4-diol + NH3
-
-
-
-
?
2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O
6-(methylamino)-1,3,5-triazine-2,4-diol + ethylamine
-
-
-
-
?
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O
6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + propan-2-amine
-
-
-
-
?
2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O
6-(methylamino)-1,3,5-triazine-2,4-diol + methylamine
-
-
-
-
?
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
6-[ethyl(methyl)amino]-1,3,5-triazine-2,4-diol + ethylamine
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + ethylamine
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + propan-2-amine
-
-
-
-
?
2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O
6-amino-1,3,5-triazine-2,4-diol + Cl-
-
-
-
-
?
2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + NH3
-
-
-
-
?
2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
6-(isopropylamino)-1,3,5-triazine-2,4-diol + NH3
-
-
-
-
?
2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + NH3
-
-
-
-
?
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + Cl-
-
-
-
-
?
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
6-(isopropylamino)-1,3,5-triazine-2,4-diol + Cl-
-
-
-
-
?
2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O
6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + 2-aminoethanol
-
-
-
-
?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
?
-
-
-
-
?
6-(ethylamino)-4-(methylamino)-1,3,5-triazin-2-ol + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + methylamine
-
-
-
-
?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + methylamine
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
cyanazine + H2O
2-{[4-(ethylamino)-6-hydroxy-1,3,5-triazin-2-yl]amino}-2-methylpropanenitrile + HCl
desethylatrazine + H2O
2-hydroxy-4-amino-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
ametryn + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H-S-CH3
-
i.e. 2-thiomethyl-4-isopropylamino-6-ethylamino-1,3,5-triazine, a s-triazine herbicide
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-
?
ametryn + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H-S-CH3
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i.e. 2-thiomethyl-4-isopropylamino-6-ethylamino-1,3,5-triazine, a s-triazine herbicide
-
-
?
atratone + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + methanol
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-
-
-
?
atratone + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + methanol
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
Clavibacter michiganese
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-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
Clavibacter michiganese ATZ1
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-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
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i.e. 2-chloro-4-isopropylamino-6-ethylamino-1,3,5-triazine, a s-triazine herbicide
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-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
i.e. 2-chloro-4-isopropylamino-6-ethylamino-1,3,5-triazine, a s-triazine herbicide
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-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
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-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
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-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
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-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
Clavibacter michiganese
-
utilization of atrazines with Cl exchanged for F, CN, N3, OCH3, SCH3
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
Clavibacter michiganese
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
Clavibacter michiganese ATZ1
-
utilization of atrazines with Cl exchanged for F, CN, N3, OCH3, SCH3
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
Clavibacter michiganese ATZ1
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
catabolic atrazine degradation pathway, overview, dechlorination
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
specific for halo-substituted triazine ring compounds
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
hydroxyatrazine + chloride
the enzyme is involved in mineralization of atrazine
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
-
-
-
-
?
cyanazine + H2O
2-{[4-(ethylamino)-6-hydroxy-1,3,5-triazin-2-yl]amino}-2-methylpropanenitrile + HCl
-
-
-
-
?
cyanazine + H2O
2-{[4-(ethylamino)-6-hydroxy-1,3,5-triazin-2-yl]amino}-2-methylpropanenitrile + HCl
-
-
-
-
?
propazine + H2O
4,6-bis(propan-2-ylamino)-1,3,5-triazin-2-ol + HCl
-
-
-
-
?
propazine + H2O
4,6-bis(propan-2-ylamino)-1,3,5-triazin-2-ol + HCl
-
-
-
-
?
simazine + H2O
4,6-diethylamino-1,3,5-triazine + HCl
-
-
-
-
?
simazine + H2O
4,6-diethylamino-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
additional information
-
-
-
following metabolites are detected, hydroxyatrazine, desisopropyl atrazine, N-isopropylammelide
-
?
additional information
?
-
AtzA can only hydrolyze triazine halides. The relatively high Km of AtzA, which is greater than the water solubility of atrazine, leads to a less efficient catalytic rate by the enzyme compared to the alternative dimeric chlorohydrolase TrzN
-
-
?
additional information
?
-
-
AtzA can only hydrolyze triazine halides. The relatively high Km of AtzA, which is greater than the water solubility of atrazine, leads to a less efficient catalytic rate by the enzyme compared to the alternative dimeric chlorohydrolase TrzN
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
catabolic atrazine degradation pathway, overview, dechlorination
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine
-
-
?
atrazine + H2O
hydroxyatrazine + chloride
the enzyme is involved in mineralization of atrazine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Zn2+
additional information
Fe2+
-
iron containing metalloenzyme, 1:1 metal to subunit stoichiometry, bound at the active site, activates
Fe2+
the AtzA hexamer contains one essential Fe2+ per monomer and active site
additional information
-
no metal ions detectable with the enzym expressed in E. coli
additional information
-
influence of and reconstitution with metals Mn2+, Fe2+, and Co2+, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
complete inhibition, reversible by addition of Fe2+, Mn2+, or Co2+
Cu2+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
Fe3+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
Ni2+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
Zn2+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
additional information
-
no inhibition by melamine and 2-chloro-4,6-diamino-S-triazine
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.02
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
-
deamination
0.04
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine
-
deamination
3.9
ametryn
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
7.8
ametryn
-
mutant R325, pH and temperature not specified in the publicationS
0.049
Atrazine
-
A216A/T217D/T219E/A220A/D250D, site-directed mutagenesis, AtzA-variant 288, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.049
Atrazine
-
T217D/T219E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.062
Atrazine
-
A216G/T217D/T219G/A220H/D250D, site-directed mutagenesis, AtzA-variant 734, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.062
Atrazine
-
A216G/T217D/T219G/A220H/D250D, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.067
Atrazine
-
A216S/T217A/T219P/A220F/D250G, site-directed mutagenesis, AtzA-variant 357, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.067
Atrazine
-
A216S/T217A/T219P/A220F/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.076
Atrazine
-
A216A/T217S/T219H/A220G/D250Y, site-directed mutagenesis, AtzA-variant 431, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.076
Atrazine
-
T217S/T219H/A220G/D250Y, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.09
Atrazine
-
A216G/T217D/T219G/A220H/D250G, site-directed mutagenesis, AtzA-variant 430, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
Atrazine
-
A216S/T217D/T219V/A220H/D250G, site-directed mutagenesis, AtzA-variant 297, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
Atrazine
-
A216Y/T217D/T219Y/A220H/D250V, site-directed mutagenesis, AtzA-variant 422, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
Atrazine
-
A216S/T217D/T219V/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.092
Atrazine
-
A216Y/T217D/T219Y/A220H/D250V, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.093
Atrazine
-
A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.093
Atrazine
-
A216Y/T217D/A220H/D250E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.095
Atrazine
-
A216G/T217D/T219A/A220V/D250W, site-directed mutagenesis, AtzA-variant 305, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.095
Atrazine
-
A216G/T217D/T219A/A220V/D250W, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.1
Atrazine
-
A216H/T217A/T219E/A220S/D250S, site-directed mutagenesis, AtzA-variant 662, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.1
Atrazine
-
A216H/T217A/T219E/A220S/D250S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.105
Atrazine
-
A216S/T217D/T219G/A220S/D250D, site-directed mutagenesis, AtzA-variant 841, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.105
Atrazine
-
A216S/T217D/T219G/A220S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.131
Atrazine
-
Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C
0.132
Atrazine
-
V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C
0.138
Atrazine
-
D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C
0.14
Atrazine
-
V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C
0.142
Atrazine
-
V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C
0.145
Atrazine
-
V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C
0.153
Atrazine
-
more than 0.153 mM. AtzA wild type, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.153
Atrazine
-
AtzA wild-type, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.9
Atrazine
-
A216G/T217D/T219G/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
3.1
Atrazine
-
mutant E241Q, pH and temperature not specified in the publication
13.3
Atrazine
-
mutant R325S, pH and temperature not specified in the publication
19
Atrazine
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
additional information
additional information
-
reduction in Km observed in the AtzA variants has allowed the full kinetic profile for the AtzA-catalyzed dechlorination of atrazine to be determined for the first time, revealing the hitherto-unreported substrate cooperativity in AtzA
-
additional information
additional information
kinetic parameters of diverse mutants relative to the wild-type values, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.2
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
-
deamination
1.4
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine
-
deamination
0.467
ametryn
-
mutant R325S, pH and temperature not specified in the publication
6.04
ametryn
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
0.0869
Atrazine
-
mutant R325S, pH and temperature not specified in the publication