Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
The taxonomic range for the selected organisms is: Pseudomonas sp.
The enzyme appears in selected viruses and cellular organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-2-haloacid + H2O
(S)-2-hydroxyacid + halide
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
?
D-2-chloropropionate + H2O
L-lactate + HCl
L-2-chloropropionate + H2O
D-lactate + HCl
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
-
-
-
?
(R)-2-chloropropionic acid + H2O
(S)-2-hydroxypropionic acid + chloride
-
-
-
-
?
(R)-2-haloacid + H2O
(S)-2-hydroxyacid + halide
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
2,2-dichloropropionate + H2O
?
-
-
-
-
?
2,3-dichloropropionate + H2O
?
-
-
-
-
?
2-chloro-3-hydroxypropionate + H2O
glycerate + HCl
-
-
-
-
?
2-chloro-n-butyrate + H2O
2-hydroxybutyrate + HCl
-
-
-
-
?
D-2-monochloropropionate + H2O
L-lactate + HCl
-
-
-
-
?
dichloroacetate + H2O
?
-
-
-
-
?
DL-2-chloropropionate + H2O
DL-lactate + HCl
-
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
-
-
-
-
?
L-2-monochloropropionate + H2O
D-lactate + HCl
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
-
-
-
-
?
monoiodoacetate + H2O
glycolate + HI
-
-
-
-
?
trichloroacetate + H2O
?
-
-
-
-
?
additional information
?
-
D-2-chloropropionate + H2O
L-lactate + HCl
-
-
-
?
D-2-chloropropionate + H2O
L-lactate + HCl
a step preceding the dehalogenation is partly rate-limiting when D-2-chloropropionate is used as the substrate
-
-
?
(R)-2-haloacid + H2O
(S)-2-hydroxyacid + halide
-
-
-
-
?
(R)-2-haloacid + H2O
(S)-2-hydroxyacid + halide
-
enzyme of 2-chloroacrylate grown cells
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
enzyme of 2-chloroacrylate grown cells
-
-
?
additional information
?
-
-
not: chloroacetamide, chloroacetaldehyde, 3-chloropropionate, best substrate: 2-halopropionate, followed by monohaloacetate, 2-halobutyrate, and 2-halovalerate in that order
-
-
?
additional information
?
-
-
the enzyme has a single common active site for both reactions on L- and D-enantiomers, the reaction mechanismm does not proceed via an ester intermediate and not via a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom on contrast to the (S)-2-haloacid dehalogenase, EC 3.8.1.2. In the (S,R)-2-haloacid dehalogenase, a water molecule directly atacks the substrate for halide displacement, structure-function relationship, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D180A
-
the mutation destabilizes the rotation of the nucleophile D10, fixes catalytic water around D10, and prevents K151 from approaching D10
D181A
-
the activity of mutant is similar to wild-type enzyme
D181E
-
the activity of mutant is similar to wild-type enzyme
D181R
-
the activity of mutant is similar to wild-type enzyme
K151A
-
the mutant is almost inactive, the mutation destabilizes substrate orientation and affects the balance of the charge around the active site
K151R
-
the mutant is almost inactive
S118A
-
the mutant retains 30% of the activity of the wild type enzyme
additional information
each of the 26 residues with charged and polar side chains, which are conserved between enzyme and D-2-haloacid dehalogenase, is mutated, Thr65, Glu69 and Asp194 are found to be essential
additional information
-
each of the 26 residues with charged and polar side chains, which are conserved between enzyme and D-2-haloacid dehalogenase, is mutated, Thr65, Glu69 and Asp194 are found to be essential
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Motosugi, K; Esahi, N.; Soda, K.
Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase
Arch. Microbiol.
131
179-183
1982
Pseudomonas sp., Pseudomonas sp. 113
brenda
Motosugi, K; Esahi, N.; Soda, K.
Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp.
J. Bacteriol.
150
522-527
1982
Pseudomonas sp., Pseudomonas sp. 113
brenda
Motosugi, K; Esahi, N.; Soda, K.
Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid
Biotechnol. Bioeng.
26
805-806
1984
Pseudomonas putida, Pseudomonas sp., Pseudomonas sp. 113
brenda
Stringfellow, J.M.; Cairns, S.S.; Cornish, A.; Cooper, R.A.
Haloalkanoate dehalogease II (DehE) of a Rhizobium sp.molecular analysis of the gene and formation of carbon monoxide from trihaloacetate by the enzyme
Eur. J. Biochem.
250
789-793
1997
Pseudomonas sp., Rhizobium sp.
brenda
Liu, J.Q.; Kurihara, T.; Hasan, A.K.M.Q.; Nardi-Dei, V.; Koshikawa, H.; Esaki, N.; Soda, K.
Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL
Appl. Environ. Microbiol.
60
2389-2393
1994
Pseudomonas sp.
brenda
Nardi-Dei, V.; Kurihara, T.; Park, C.; Miyagi, M.; Tsunasawa, S.; Soda, K.; Esaki, N.
DL-2-Haloacid dehalogenase from Pseudomonas sp. 113 is a new class of dehalogenase catalyzing hydrolytic dehalogenation not involving enzyme-substrate ester intermediate
J. Biol. Chem.
274
20977-20981
1999
Pseudomonas sp., Pseudomonas sp. 113
brenda
Soda, K.; Kurihara, T.; Liu, J.Q.; Nardi-Dei, V.; Park, C.; Miyagi, M.; Tsunasawa, S.; Esaki, N.
Bacterial 2-haloacid dehalogenases: Structures and catalytic properties
Pure Appl. Chem.
68
2097-2103
1996
Pseudomonas putida, Pseudomonas putida PP3, Pseudomonas sp., Pseudomonas sp. 113, Rhizobium sp.
-
brenda
Kurihara, T.; Esaki, N.; Soda, K.
Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms
J. Mol. Catal. B
10
57-65
2000
Pseudomonas sp., Pseudomonas sp. 113
-
brenda
Nardi-Dei, V.; Kurihara, T.; Park, C.; Esaki, N.; Soda, K.
Bacterial DL-2-haloacid dehalogenase from Pseudomonas sp. strain 113: gene cloning and structural comparison with D- and L-2-haloacid dehalogenases
J. Bacteriol.
179
4232-4238
1997
Achromobacter xylosoxidans (Q59168), Pseudomonas putida, Pseudomonas putida AJ1, Pseudomonas sp. (O06652), Pseudomonas sp., Pseudomonas sp. 113 (O06652)
brenda
Ohkouchi, Y.; Koshikawa, H.; Terashima, Y.
Cloning and expression of DL-2-haloacid dehalogenase gene from Burkholderia cepacia
Water Sci. Technol.
42
261-268
2000
Burkholderia cepacia, Burkholderia cepacia KY, Pseudomonas sp., Pseudomonas sp. 113
-
brenda
Hasan, A.K.M.Q.; Takada, H.; Koshikawa, H.; Liu, J.Q.; Kurihara, T.; Esaki, N.; Soda, K.
Two kinds of 2-halo acid dehalogeases fromm Pseudomonas sp. YL induced by 2-chloroacrylates and 2-chloropropionate
Biosci. Biotechnol. Biochem.
58
1599-1602
1994
Pseudomonas sp.
-
brenda
Kurihara, T.; Esaki, N.
Bacterial hydrolytic dehalogenases and related enzymes: occurrences, reaction mechanisms, and applications
Chem. Rec.
8
67-74
2008
Methylobacterium sp. CPA1 (A6BM74), Pseudomonas putida, Pseudomonas putida PP3, Pseudomonas sp. (O06652), Pseudomonas sp. 113 (O06652)
brenda
Nakamura, T.; Yamaguchi, A.; Kondo, H.; Watanabe, H.; Kurihara, T.; Esaki, N.; Hirono, S.; Tanaka, S.
Roles of K151 and D180 in L-2-haloacid dehalogenase from Pseudomonas sp. YL: Analysis by molecular dynamics and ab initio fragment molecular orbital calculations
J. Comput. Chem.
30
2625-2634
2009
Pseudomonas sp.
brenda
Kurihara, T.
A mechanistic analysis of enzymatic degradation of organohalogen compounds
Biosci. Biotechnol. Biochem.
75
189-198
2011
Pseudomonas sp., Pseudomonas sp. 113
brenda