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IUBMB Comments Highly specific; does not act on pyruvate, oxaloacetate, maleylpyruvate, fumarylpyruvate or acetylacetone.
Synonyms
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acetopyruvate hydrolase
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acetopyruvate hydrolase
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acetylpyruvate + H2O = acetate + pyruvate
does not act on pyruvate, oxaloacetate, maleylpyruvate, fumarylpyruvate or acetylacetone
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hydrolysis of C-C bond
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MetaCyc
orcinol degradation
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2,4-dioxopentanoate acetylhydrolase
Highly specific; does not act on pyruvate, oxaloacetate, maleylpyruvate, fumarylpyruvate or acetylacetone.
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acetylpyruvate + H2O
acetate + pyruvate
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Substrates: plays a role in the orcinol catabolism
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acetopyruvate + H2O
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Substrates: -
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acetopyruvate + H2O
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Substrates: -
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acetylpyruvate + H2O
acetate + pyruvate
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Substrates: plays a role in the orcinol catabolism
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oxalacetate
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competitive inhibition, 0.2-1 mM
oxalate
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competitive inhibition, 2-6 mM
pyruvate
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competitive inhibition, 2-10 mM
Cu2+
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orcinol
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inducer for enzyme production
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brenda
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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Q9VDE2_DROME
220
0
23619
TrEMBL
other Location (Reliability: 3 )
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monomer
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1 * 38000, SDS-PAGE
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additional information
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75fold increased productivity of the enzyme by media optimization, optimal glucose, yeast extract and (NH4)2SO4 concentrations produce 7fold more biomass than the initial medium, optimization also by determining the optimal time of feed and amount of orcinol
additional information
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75fold increased productivity of the enzyme by media optimization, optimal glucose, yeast extract and (NH4)2SO4 concentrations produce 7fold more biomass than the initial medium, optimization also by determining the optimal time of feed and amount of orcinol
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Davey, J.F.; Ribbons, D.W.
Metabolism of resorcinylic compounds by bacteria. Purification and properties of acetylpyruvate hydrolase from Pseudomonas putida 01
J. Biol. Chem.
250
3826-3830
1975
Pseudomonas putida
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Pokorny, D.; Brecker, L.; Pogorevc, M.; Steiner, W.; Griengl, H.; Kappe, T.; Ribbons, D.W.
Proton-nuclear magnetic resonance analyses of the substrates specificity of a beta-ketolase from Pseudomonas putida, acetopyruvate hydrolase
J. Bacteriol.
181
5051-5059
1999
Pseudomonas putida
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Hofer, H.; Mandl, T.; Steiner, W.
Acetopyruvate hydrolase production by Pseudomonas putida O1 - optimization of batch and fed-batch fermentations
Appl. Microbiol. Biotechnol.
60
293-299
2002
Pseudomonas putida, Pseudomonas putida O1
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