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Information on EC 3.6.5.5 - dynamin GTPase and Organism(s) Bos taurus and UniProt Accession A6H7I5
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3.6.5.5 dynamin GTPaseIUBMB Comments
An enzyme with a molecular mass of about 100 kDa that is involved in endocytosis and is instrumental in pinching off membrane vesicles.
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Word Map
- 3.6.5.5
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endocytosis
- gtpases
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endocytic
- clathrin
- actin
- synaptic
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clathrin-mediated
- endosomes
-
dominant-negative
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clathrin-coated
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mitofusins
- cytoskeleton
- dynasore
- pi
- tubule
-
scission
-
mitophagy
-
constrict
- microtubule
- transferrin
-
receptor-mediated
- adaptor
- synapses
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presynaptic
- myopathy
- rab5
- pink1
-
caveolae
-
brachytherapy
-
gtp-binding
- src
-
mechanochemical
-
clathrin-independent
- eps15
- caveolin-1
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macropinocytosis
- parkin
- charcot-marie-tooth
-
cristae
-
pleckstrin
-
invagination
- arf6
- agriculture
- arrestins
- medicine
-
pten-induced
-
n-wasp
-
dosimetric
- cortactin
-
dose-volume
- grb2
- beta-arrestins
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
dynamin, dynamin-related protein 1, dynamin 2, d100, dynamin-2, dynamin 1, dynamin i, optic atrophy 1, dynamin-1, gtpase dynamin, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
B-dynamin
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-
-
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D100
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-
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Dynamin BREDNM19
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-
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Dynamin UDNM
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-
-
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Dynamin, brain
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-
-
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Dynamin, testicular
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-
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GTP phosphohydrolase
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-
-
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GTPase
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-
-
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guanine triphosphatase
-
-
-
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guanosine 5'-triphosphatase
-
-
-
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guanosine triphosphatase
-
-
-
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phosphatase, guanosine tri-
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-
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ribosomal GTPase
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-
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Shibire protein
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-
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T-dynamin
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GTP + H2O = GDP + phosphate
an enzyme with a molecular mass of about 100 kDa that is involved in endocytosis and is instrumental in pinching off membrane vesicles
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
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-
-
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SYSTEMATIC NAME
IUBMB Comments
GTP phosphohydrolase (vesicle-releasing)
An enzyme with a molecular mass of about 100 kDa that is involved in endocytosis and is instrumental in pinching off membrane vesicles.
CAS REGISTRY NUMBER
COMMENTARY
9059-32-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
-
dynamin self-association is not sufficient condition for the expression of maximal GTPase activity, dynamin molecules must be in proper conformation or orientation if they are to form an active oligomer
-
?
GTP + H2O
GDP + phosphate
-
critical step in fission of coated pits to form coated vesicles
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?
GTP + H2O
GDP + phosphate
-
enzyme is essential for budding clathrin-coated vesicles from the plasma membrane
-
?
GTP + H2O
GDP + phosphate
-
plays an essential role in synaptic vesicle recycling
-
?
GTP + H2O
GDP + phosphate
-
plays an essential role in synaptic vesicle recycling
-
?
GTP + H2O
GDP + phosphate
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involved in clathrin-mediated endocytosis and other vesicular trafficking processes, dynamin has a mechanochemical function in vesicle scission, the GTPase activity of dynamin and the resulting conformation change are essential for endocytosis
-
-
?
GTP + H2O
GDP + phosphate
-
GTP hydrolysis results in conformational changes in dynamin bound to lipid nanotubes
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
-
critical step in fission of coated pits to form coated vesicles
-
?
GTP + H2O
GDP + phosphate
-
enzyme is essential for budding clathrin-coated vesicles from the plasma membrane
-
?
GTP + H2O
GDP + phosphate
-
plays an essential role in synaptic vesicle recycling
-
?
GTP + H2O
GDP + phosphate
-
plays an essential role in synaptic vesicle recycling
-
?
GTP + H2O
GDP + phosphate
-
involved in clathrin-mediated endocytosis and other vesicular trafficking processes, dynamin has a mechanochemical function in vesicle scission, the GTPase activity of dynamin and the resulting conformation change are essential for endocytosis
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cyclosporin A
treating BL-3 cells with 0.005 mM cyclosporine depletes mitochondrial dynamin-2
heterotrimeric G protein betagamma subunit complex
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oligomerization of dynamin prevents inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4,5-triphosphate
anionic liposome
-
potent stimulation of dynamin GTPase activity at low ionic strength, activator interacts with the highly basic C-terminal proline/arginine-rich domain of dynamin via ionic interactions that are essentially abrogated at physiologic ionic strength
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Grb2
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activates, binds to carboxyl-terminal proline/arginine-rich domain. Grb2 and phosphatidylinositol 4,5-bisphosphate together increase the dynamin GTPase activity up to 4fold higher than that obtained by these activators tested separately
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microtubule
-
potent stimulation of dynamin GTPase activity at low ionic strength, activator interacts with the highly basic C-terminal proline/arginine-rich domain of dynamin via ionic interactions that are essentially abrogated at physiologic ionic strength
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microtubules
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activates, binds to carboxyl-terminal proline/arginine-rich domain
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phosphatidylinositol 3,4-bisphosphate
-
weak activation, binds to the pleckstrin homology domain
phosphatidylinositol 4,5-bisphosphate
-
activates the GTPase activity of dynamin, specific for D-5 phosphoinositides, activation mechanism, activates also in the presence of 100-130 mM NaCl, the interactions are mediated by the dynamin pleckstrin homology domain
SH3 domain-containing protein
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activates the GTPase activity of dynamin, activation mechanism
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1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4,5-triphosphate
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activates, binds to the pleckstrin homology domain
1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4,5-triphosphate
-
activates the GTPase activity of dynamin, specific for D-5 phosphoinositides, activation mechanism, activates also in the presence of 100-130 mM NaCl, the interactions are mediated by the dynamin pleckstrin homology domain
phosphatidylinositol-4,5-bisphosphate
-
best activator, binds to the pleckstrin homology domain. Grb2 and phosphatidylinositol 4,5-bisphosphate together increase the dynamin GTPase activity up to 4fold higher than that obtained by these activators tested separately
additional information
-
dynamin is activated by the facilitation of its self-association, GTPase activity is stimulated by any factor that induces the formation of appropriately oriented dynamin polymers, not activated by phosphatidylinositol 4-phosphate or 1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4-diphosphate
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additional information
-
oligomerization of dynamin stimulates its GTPase activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.005 - 0.05
GTP
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Km is in the range of 0.005-0.05 mM depending on salt and temperature conditions
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
leukotoxin mobilizes dynamin-2 from mitochondria to the cell membrane
mitochondria are the principal source of dynamin-2 in BL-3 cells
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DYN2_BOVIN
866
0
97836
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
dynamin is characterized by a tetramer/monomer equilibrium. Dynamin molecules must be in proper conformation or orientation if they are to form an active oligomer
additional information
-
oligomerization of dynamin stimulates its GTPase activity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K142A
-
mutation in the GTPase domain of dynamin, mutant has a moderately impaired GTPase activity, kinetics
S45N
-
mutation in the GTPase domain of dynamin, mutant is defective in GTP binding, kinetics
T141Q
-
mutation in the GTPase domain of dynamin, mutant is partially inhibited in its GTPase activity, kinetics
T65A
-
mutation in the GTPase domain of dynamin, very low GTPase activity, kinetics
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
wild-type and mutant dynamin is expressed using the baculovirus pBac 4 system, expression in COS-7 cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Binns, D.D.; Barylko, B.; Grichine, N.; Atkinson, M.A.L.; Helms, M.K.; Jameson, D.M.; Eccleston, J.F.; Albanesi, J.P.
Correlation between self-association modes and GTPase activation of dynamin
J. Protein Chem.
18
277-290
1999
Bos taurus
Lin, H.C.; Gilman, A.G.
Regulation of dynamin I GTPase activity by G protein betagamma subunits and phosphatidylinositol 4,5-bisphosphate
J. Biol. Chem.
271
27979-27982
1996
Bos taurus, Homo sapiens
Shpetner, H.S.; Vallee, R.B.
Dynamin is a GTPase stimulated to high levels of activity by microtubules
Nature
355
733-735
1992
Bos taurus
Barylko, B.; Binns, D.; Lin, K.M.; Atkinson, M.A.L.; Jameson, D.M.; Yin, H.L.; Albanesi, J.P.
Synergistic activation of dynamin GTPase by Grb2 and phosphoinositides
J. Biol. Chem.
273
3791-3797
1998
Bos taurus
Barylko, B.; Binns, D.D.; Albanesi, J.P.
Activation of dynamin GTPase activity by phosphoinositides and SH3 domain-containing proteins
Methods Enzymol.
329
486-496
2001
Bos taurus
Marks, B.; Stowell, M.H.B.; Vallis, Y.; Mills, I.G.; Gibson, A.; Hopkins, C.R.; McMahon, H.T.
GTPase activity of dynamin and resulting conformation change are essential for endocytosis
Nature
410
231-235
2001
Bos taurus
Yoshida, Y.; Takei, K.
Stimulation of dynamin GTPase activity by amphiphysin
Methods Enzymol.
404
528-537
2005
Bos taurus
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